Strain Fitness in Methanococcus maripaludis S2 around MMP_RS06380

Experiment: H2 N-free with 10mM L-Aspartic Acid

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMP_RS06370 and MMP_RS06375 overlap by 4 nucleotidesMMP_RS06375 and MMP_RS06380 are separated by 27 nucleotidesMMP_RS06380 and MMP_RS06385 are separated by 5 nucleotidesMMP_RS06385 and MMP_RS06390 are separated by 10 nucleotides MMP_RS06370: MMP_RS06370 - biotin synthase BioB, at 1,223,883 to 1,224,866 _RS06370 MMP_RS06375: MMP_RS06375 - ATP-dependent sacrificial sulfur transferase LarE, at 1,224,863 to 1,225,639 _RS06375 MMP_RS06380: MMP_RS06380 - O-phospho-L-seryl-tRNA:Cys-tRNA synthase, at 1,225,667 to 1,226,809 _RS06380 MMP_RS06385: MMP_RS06385 - hypothetical protein, at 1,226,815 to 1,227,174 _RS06385 MMP_RS06390: MMP_RS06390 - quinolinate synthase NadA, at 1,227,185 to 1,228,102 _RS06390 Position (kb) 1225 1226 1227Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 1224.897 kb on + strandat 1224.933 kb on - strandat 1224.971 kb on + strand, within MMP_RS06375at 1224.971 kb on + strand, within MMP_RS06375at 1225.246 kb on + strand, within MMP_RS06375at 1225.378 kb on - strand, within MMP_RS06375at 1225.485 kb on + strand, within MMP_RS06375at 1225.570 kb on + strandat 1225.582 kb on - strandat 1225.902 kb on - strandat 1226.409 kb on - strandat 1226.873 kb on + strand, within MMP_RS06385at 1226.978 kb on - strand, within MMP_RS06385at 1226.990 kb on + strand, within MMP_RS06385at 1227.074 kb on - strand, within MMP_RS06385at 1227.075 kb on + strand, within MMP_RS06385at 1227.080 kb on - strand, within MMP_RS06385at 1227.173 kb on + strandat 1227.710 kb on + strandat 1227.766 kb on - strandat 1227.771 kb on + strandat 1227.779 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid
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1,224,897 + -2.4
1,224,933 - -1.6
1,224,971 + MMP_RS06375 0.14 -2.8
1,224,971 + MMP_RS06375 0.14 -3.3
1,225,246 + MMP_RS06375 0.49 -1.4
1,225,378 - MMP_RS06375 0.66 -2.2
1,225,485 + MMP_RS06375 0.80 -4.5
1,225,570 + -1.3
1,225,582 - -3.4
1,225,902 - -1.1
1,226,409 - -2.8
1,226,873 + MMP_RS06385 0.16 +0.3
1,226,978 - MMP_RS06385 0.45 -0.3
1,226,990 + MMP_RS06385 0.49 -0.5
1,227,074 - MMP_RS06385 0.72 +0.0
1,227,075 + MMP_RS06385 0.72 +0.1
1,227,080 - MMP_RS06385 0.74 -0.4
1,227,173 + +0.1
1,227,710 + -2.9
1,227,766 - -1.2
1,227,771 + -1.1
1,227,779 - -3.2

Or see this region's nucleotide sequence