Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06370 and MMP_RS06375 overlap by 4 nucleotides MMP_RS06375 and MMP_RS06380 are separated by 27 nucleotides MMP_RS06380 and MMP_RS06385 are separated by 5 nucleotides MMP_RS06385 and MMP_RS06390 are separated by 10 nucleotides
MMP_RS06370: MMP_RS06370 - biotin synthase BioB, at 1,223,883 to 1,224,866
_RS06370
MMP_RS06375: MMP_RS06375 - ATP-dependent sacrificial sulfur transferase LarE, at 1,224,863 to 1,225,639
_RS06375
MMP_RS06380: MMP_RS06380 - O-phospho-L-seryl-tRNA:Cys-tRNA synthase, at 1,225,667 to 1,226,809
_RS06380
MMP_RS06385: MMP_RS06385 - hypothetical protein, at 1,226,815 to 1,227,174
_RS06385
MMP_RS06390: MMP_RS06390 - quinolinate synthase NadA, at 1,227,185 to 1,228,102
_RS06390
Position (kb)
1225
1226
1227 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1224.897 kb on + strand at 1224.933 kb on - strand at 1224.971 kb on + strand, within MMP_RS06375 at 1224.971 kb on + strand, within MMP_RS06375 at 1225.246 kb on + strand, within MMP_RS06375 at 1225.378 kb on - strand, within MMP_RS06375 at 1225.485 kb on + strand, within MMP_RS06375 at 1225.570 kb on + strand at 1225.582 kb on - strand at 1225.902 kb on - strand at 1226.409 kb on - strand at 1226.873 kb on + strand, within MMP_RS06385 at 1226.978 kb on - strand, within MMP_RS06385 at 1226.990 kb on + strand, within MMP_RS06385 at 1227.074 kb on - strand, within MMP_RS06385 at 1227.075 kb on + strand, within MMP_RS06385 at 1227.080 kb on - strand, within MMP_RS06385 at 1227.173 kb on + strand at 1227.710 kb on + strand at 1227.766 kb on - strand at 1227.771 kb on + strand at 1227.779 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 1,224,897 + -2.4 1,224,933 - -1.6 1,224,971 + MMP_RS06375 0.14 -2.8 1,224,971 + MMP_RS06375 0.14 -3.3 1,225,246 + MMP_RS06375 0.49 -1.4 1,225,378 - MMP_RS06375 0.66 -2.2 1,225,485 + MMP_RS06375 0.80 -4.5 1,225,570 + -1.3 1,225,582 - -3.4 1,225,902 - -1.1 1,226,409 - -2.8 1,226,873 + MMP_RS06385 0.16 +0.3 1,226,978 - MMP_RS06385 0.45 -0.3 1,226,990 + MMP_RS06385 0.49 -0.5 1,227,074 - MMP_RS06385 0.72 +0.0 1,227,075 + MMP_RS06385 0.72 +0.1 1,227,080 - MMP_RS06385 0.74 -0.4 1,227,173 + +0.1 1,227,710 + -2.9 1,227,766 - -1.2 1,227,771 + -1.1 1,227,779 - -3.2
Or see this region's nucleotide sequence