Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06320 and MMP_RS06325 are separated by 94 nucleotides MMP_RS06325 and MMP_RS06330 are separated by 72 nucleotides MMP_RS06330 and MMP_RS06335 are separated by 94 nucleotides MMP_RS06335 and MMP_RS06340 are separated by 20 nucleotides
MMP_RS06320: MMP_RS06320 - hypothetical protein, at 1,217,265 to 1,217,903
_RS06320
MMP_RS06325: MMP_RS06325 - hypothetical protein, at 1,217,998 to 1,218,300
_RS06325
MMP_RS06330: MMP_RS06330 - nucleotidyltransferase domain-containing protein, at 1,218,373 to 1,219,431
_RS06330
MMP_RS06335: MMP_RS06335 - HAD family hydrolase, at 1,219,526 to 1,219,909
_RS06335
MMP_RS06340: MMP_RS06340 - phosphoadenosine phosphosulfate reductase family protein, at 1,219,930 to 1,220,820
_RS06340
Position (kb)
1218
1219
1220 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1217.386 kb on - strand, within MMP_RS06320 at 1217.391 kb on + strand, within MMP_RS06320 at 1217.396 kb on - strand, within MMP_RS06320 at 1217.396 kb on - strand, within MMP_RS06320 at 1217.397 kb on + strand, within MMP_RS06320 at 1217.401 kb on - strand, within MMP_RS06320 at 1217.472 kb on - strand, within MMP_RS06320 at 1217.612 kb on - strand, within MMP_RS06320 at 1217.826 kb on - strand, within MMP_RS06320 at 1217.864 kb on - strand at 1217.901 kb on - strand at 1217.963 kb on - strand at 1217.966 kb on - strand at 1217.992 kb on - strand at 1217.993 kb on + strand at 1218.004 kb on + strand at 1218.005 kb on - strand at 1218.070 kb on + strand, within MMP_RS06325 at 1218.313 kb on - strand at 1218.314 kb on + strand at 1218.316 kb on + strand at 1218.316 kb on + strand at 1218.319 kb on - strand at 1218.320 kb on + strand at 1218.320 kb on + strand at 1218.384 kb on - strand at 1218.476 kb on - strand at 1218.536 kb on - strand at 1219.097 kb on - strand at 1219.123 kb on - strand at 1219.291 kb on - strand at 1219.411 kb on - strand at 1219.448 kb on - strand at 1219.460 kb on + strand at 1219.546 kb on + strand at 1219.589 kb on - strand, within MMP_RS06335 at 1219.606 kb on - strand, within MMP_RS06335 at 1219.718 kb on - strand, within MMP_RS06335 at 1219.719 kb on + strand, within MMP_RS06335 at 1219.987 kb on - strand at 1220.075 kb on + strand, within MMP_RS06340 at 1220.173 kb on - strand, within MMP_RS06340 at 1220.211 kb on - strand, within MMP_RS06340 at 1220.326 kb on + strand, within MMP_RS06340 at 1220.352 kb on + strand, within MMP_RS06340 at 1220.359 kb on - strand, within MMP_RS06340 at 1220.404 kb on - strand, within MMP_RS06340
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 1,217,386 - MMP_RS06320 0.19 +0.3 1,217,391 + MMP_RS06320 0.20 +0.9 1,217,396 - MMP_RS06320 0.21 -0.0 1,217,396 - MMP_RS06320 0.21 -0.0 1,217,397 + MMP_RS06320 0.21 -0.6 1,217,401 - MMP_RS06320 0.21 -0.2 1,217,472 - MMP_RS06320 0.32 +0.4 1,217,612 - MMP_RS06320 0.54 +1.2 1,217,826 - MMP_RS06320 0.88 +1.1 1,217,864 - -0.5 1,217,901 - -0.1 1,217,963 - -1.0 1,217,966 - -0.5 1,217,992 - -1.6 1,217,993 + +0.3 1,218,004 + +0.8 1,218,005 - -0.5 1,218,070 + MMP_RS06325 0.24 +0.1 1,218,313 - -0.8 1,218,314 + -0.4 1,218,316 + +0.5 1,218,316 + -0.7 1,218,319 - -1.1 1,218,320 + -0.4 1,218,320 + +0.3 1,218,384 - -2.5 1,218,476 - -3.1 1,218,536 - -4.9 1,219,097 - -4.6 1,219,123 - -5.3 1,219,291 - -3.4 1,219,411 - -3.5 1,219,448 - -1.6 1,219,460 + -0.9 1,219,546 + -0.4 1,219,589 - MMP_RS06335 0.16 +0.4 1,219,606 - MMP_RS06335 0.21 +0.5 1,219,718 - MMP_RS06335 0.50 +0.0 1,219,719 + MMP_RS06335 0.50 -0.3 1,219,987 - -2.8 1,220,075 + MMP_RS06340 0.16 -2.1 1,220,173 - MMP_RS06340 0.27 -2.1 1,220,211 - MMP_RS06340 0.32 -2.1 1,220,326 + MMP_RS06340 0.44 -2.2 1,220,352 + MMP_RS06340 0.47 -0.6 1,220,359 - MMP_RS06340 0.48 -0.7 1,220,404 - MMP_RS06340 0.53 -1.4
Or see this region's nucleotide sequence