Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06235 and MMP_RS06240 are separated by 18 nucleotides MMP_RS06240 and MMP_RS06245 are separated by 38 nucleotides MMP_RS06245 and MMP_RS06250 are separated by 41 nucleotides
MMP_RS06235: MMP_RS06235 - hydroxymethylglutaryl-CoA synthase, at 1,198,055 to 1,199,104
_RS06235
MMP_RS06240: MMP_RS06240 - thiolase domain-containing protein, at 1,199,123 to 1,200,301
_RS06240
MMP_RS06245: MMP_RS06245 - Zn-ribbon domain-containing OB-fold protein, at 1,200,340 to 1,200,732
_RS06245
MMP_RS06250: MMP_RS06250 - hypothetical protein, at 1,200,774 to 1,201,451
_RS06250
Position (kb)
1199
1200
1201 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1198.458 kb on + strand at 1199.556 kb on + strand at 1199.556 kb on + strand at 1199.650 kb on - strand at 1199.888 kb on + strand at 1200.236 kb on + strand at 1200.312 kb on + strand at 1200.766 kb on + strand at 1200.776 kb on + strand at 1200.780 kb on + strand at 1200.826 kb on - strand at 1200.857 kb on + strand, within MMP_RS06250 at 1200.917 kb on - strand, within MMP_RS06250 at 1201.002 kb on - strand, within MMP_RS06250 at 1201.150 kb on + strand, within MMP_RS06250 at 1201.279 kb on + strand, within MMP_RS06250
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 1,198,458 + -3.3 1,199,556 + -1.5 1,199,556 + -1.9 1,199,650 - -4.8 1,199,888 + -1.3 1,200,236 + -0.1 1,200,312 + -2.9 1,200,766 + +0.4 1,200,776 + -0.8 1,200,780 + -0.0 1,200,826 - -0.7 1,200,857 + MMP_RS06250 0.12 -0.2 1,200,917 - MMP_RS06250 0.21 -0.8 1,201,002 - MMP_RS06250 0.34 -0.1 1,201,150 + MMP_RS06250 0.55 -0.5 1,201,279 + MMP_RS06250 0.74 +0.2
Or see this region's nucleotide sequence