Strain Fitness in Methanococcus maripaludis S2 around MMP_RS06235

Experiment: H2 N-free with 10mM L-Aspartic Acid

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMP_RS06225 and MMP_RS06230 are separated by 135 nucleotidesMMP_RS06230 and MMP_RS06235 are separated by 62 nucleotidesMMP_RS06235 and MMP_RS06240 are separated by 18 nucleotides MMP_RS06225: MMP_RS06225 - DNA double-strand break repair nuclease NurA, at 1,196,103 to 1,197,167 _RS06225 MMP_RS06230: MMP_RS06230 - helix-turn-helix domain-containing protein, at 1,197,303 to 1,197,992 _RS06230 MMP_RS06235: MMP_RS06235 - hydroxymethylglutaryl-CoA synthase, at 1,198,055 to 1,199,104 _RS06235 MMP_RS06240: MMP_RS06240 - thiolase domain-containing protein, at 1,199,123 to 1,200,301 _RS06240 Position (kb) 1198 1199 1200Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2at 1197.166 kb on + strandat 1197.170 kb on - strandat 1197.319 kb on - strandat 1197.499 kb on + strand, within MMP_RS06230at 1197.502 kb on - strand, within MMP_RS06230at 1197.502 kb on - strand, within MMP_RS06230at 1197.504 kb on - strand, within MMP_RS06230at 1197.505 kb on + strand, within MMP_RS06230at 1197.543 kb on + strand, within MMP_RS06230at 1197.764 kb on + strand, within MMP_RS06230at 1197.928 kb on - strandat 1197.935 kb on - strandat 1198.075 kb on + strandat 1198.458 kb on + strandat 1199.556 kb on + strandat 1199.556 kb on + strandat 1199.650 kb on - strandat 1199.888 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid
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1,197,166 + +0.3
1,197,170 - -0.7
1,197,319 - -1.4
1,197,499 + MMP_RS06230 0.28 -0.4
1,197,502 - MMP_RS06230 0.29 +0.4
1,197,502 - MMP_RS06230 0.29 -0.5
1,197,504 - MMP_RS06230 0.29 +0.5
1,197,505 + MMP_RS06230 0.29 -2.4
1,197,543 + MMP_RS06230 0.35 -1.6
1,197,764 + MMP_RS06230 0.67 -2.4
1,197,928 - +2.1
1,197,935 - -1.7
1,198,075 + -0.7
1,198,458 + -3.3
1,199,556 + -1.5
1,199,556 + -1.9
1,199,650 - -4.8
1,199,888 + -1.3

Or see this region's nucleotide sequence