Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06225 and MMP_RS06230 are separated by 135 nucleotides MMP_RS06230 and MMP_RS06235 are separated by 62 nucleotides MMP_RS06235 and MMP_RS06240 are separated by 18 nucleotides
MMP_RS06225: MMP_RS06225 - DNA double-strand break repair nuclease NurA, at 1,196,103 to 1,197,167
_RS06225
MMP_RS06230: MMP_RS06230 - helix-turn-helix domain-containing protein, at 1,197,303 to 1,197,992
_RS06230
MMP_RS06235: MMP_RS06235 - hydroxymethylglutaryl-CoA synthase, at 1,198,055 to 1,199,104
_RS06235
MMP_RS06240: MMP_RS06240 - thiolase domain-containing protein, at 1,199,123 to 1,200,301
_RS06240
Position (kb)
1198
1199
1200 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1197.166 kb on + strand at 1197.170 kb on - strand at 1197.319 kb on - strand at 1197.499 kb on + strand, within MMP_RS06230 at 1197.502 kb on - strand, within MMP_RS06230 at 1197.502 kb on - strand, within MMP_RS06230 at 1197.504 kb on - strand, within MMP_RS06230 at 1197.505 kb on + strand, within MMP_RS06230 at 1197.543 kb on + strand, within MMP_RS06230 at 1197.764 kb on + strand, within MMP_RS06230 at 1197.928 kb on - strand at 1197.935 kb on - strand at 1198.075 kb on + strand at 1198.458 kb on + strand at 1199.556 kb on + strand at 1199.556 kb on + strand at 1199.650 kb on - strand at 1199.888 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 1,197,166 + +0.3 1,197,170 - -0.7 1,197,319 - -1.4 1,197,499 + MMP_RS06230 0.28 -0.4 1,197,502 - MMP_RS06230 0.29 +0.4 1,197,502 - MMP_RS06230 0.29 -0.5 1,197,504 - MMP_RS06230 0.29 +0.5 1,197,505 + MMP_RS06230 0.29 -2.4 1,197,543 + MMP_RS06230 0.35 -1.6 1,197,764 + MMP_RS06230 0.67 -2.4 1,197,928 - +2.1 1,197,935 - -1.7 1,198,075 + -0.7 1,198,458 + -3.3 1,199,556 + -1.5 1,199,556 + -1.9 1,199,650 - -4.8 1,199,888 + -1.3
Or see this region's nucleotide sequence