Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06210 and MMP_RS06215 are separated by 209 nucleotides MMP_RS06215 and MMP_RS06220 are separated by 161 nucleotides MMP_RS06220 and MMP_RS06225 are separated by 36 nucleotides
MMP_RS06210: MMP_RS06210 - type I glutamate--ammonia ligase, at 1,192,748 to 1,194,088
_RS06210
MMP_RS06215: MMP_RS06215 - 30S ribosomal protein S6e, at 1,194,298 to 1,194,672
_RS06215
MMP_RS06220: MMP_RS06220 - translation initiation factor IF-2 subunit gamma, at 1,194,834 to 1,196,066
_RS06220
MMP_RS06225: MMP_RS06225 - DNA double-strand break repair nuclease NurA, at 1,196,103 to 1,197,167
_RS06225
Position (kb)
1194
1195
1196
1197 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1194.105 kb on + strand at 1194.116 kb on + strand at 1194.187 kb on + strand at 1194.192 kb on - strand at 1194.271 kb on - strand at 1194.271 kb on - strand at 1194.272 kb on + strand at 1194.510 kb on + strand, within MMP_RS06215 at 1194.510 kb on + strand, within MMP_RS06215 at 1194.597 kb on - strand, within MMP_RS06215 at 1194.719 kb on - strand at 1194.720 kb on + strand at 1194.721 kb on - strand at 1194.722 kb on + strand at 1194.724 kb on + strand at 1194.727 kb on - strand at 1194.727 kb on - strand at 1194.727 kb on - strand at 1194.728 kb on + strand at 1195.083 kb on + strand at 1195.513 kb on - strand at 1195.801 kb on - strand at 1196.138 kb on - strand at 1196.140 kb on - strand at 1196.140 kb on - strand at 1196.141 kb on + strand at 1196.141 kb on + strand at 1196.267 kb on - strand, within MMP_RS06225 at 1196.270 kb on + strand, within MMP_RS06225 at 1196.286 kb on + strand, within MMP_RS06225 at 1196.404 kb on + strand, within MMP_RS06225 at 1196.404 kb on + strand, within MMP_RS06225 at 1196.415 kb on + strand, within MMP_RS06225 at 1196.497 kb on - strand, within MMP_RS06225 at 1196.525 kb on - strand, within MMP_RS06225 at 1196.562 kb on - strand, within MMP_RS06225 at 1196.711 kb on + strand, within MMP_RS06225 at 1196.823 kb on + strand, within MMP_RS06225 at 1196.981 kb on - strand, within MMP_RS06225 at 1196.998 kb on - strand, within MMP_RS06225
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 1,194,105 + -0.5 1,194,116 + -1.4 1,194,187 + +1.7 1,194,192 - -1.6 1,194,271 - +0.8 1,194,271 - -0.2 1,194,272 + +0.6 1,194,510 + MMP_RS06215 0.57 -0.1 1,194,510 + MMP_RS06215 0.57 +0.5 1,194,597 - MMP_RS06215 0.80 -0.3 1,194,719 - +0.3 1,194,720 + +1.2 1,194,721 - +0.1 1,194,722 + +0.2 1,194,724 + +0.5 1,194,727 - -1.9 1,194,727 - -1.1 1,194,727 - -1.0 1,194,728 + -1.2 1,195,083 + -0.2 1,195,513 - -0.9 1,195,801 - -2.8 1,196,138 - +0.3 1,196,140 - +0.1 1,196,140 - +0.2 1,196,141 + -0.2 1,196,141 + -0.8 1,196,267 - MMP_RS06225 0.15 -0.2 1,196,270 + MMP_RS06225 0.16 -0.2 1,196,286 + MMP_RS06225 0.17 -1.6 1,196,404 + MMP_RS06225 0.28 +0.1 1,196,404 + MMP_RS06225 0.28 -0.7 1,196,415 + MMP_RS06225 0.29 +0.2 1,196,497 - MMP_RS06225 0.37 +0.1 1,196,525 - MMP_RS06225 0.40 +0.4 1,196,562 - MMP_RS06225 0.43 +0.1 1,196,711 + MMP_RS06225 0.57 -1.0 1,196,823 + MMP_RS06225 0.68 +1.1 1,196,981 - MMP_RS06225 0.82 +0.2 1,196,998 - MMP_RS06225 0.84 +0.3
Or see this region's nucleotide sequence