Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06185 and MMP_RS06190 are separated by 9 nucleotides MMP_RS06190 and MMP_RS06195 are separated by 7 nucleotides MMP_RS06195 and MMP_RS06200 are separated by 39 nucleotides
MMP_RS06185: MMP_RS06185 - thermonuclease family protein, at 1,188,095 to 1,188,652
_RS06185
MMP_RS06190: MMP_RS06190 - tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO, at 1,188,662 to 1,189,045
_RS06190
MMP_RS06195: MMP_RS06195 - cobalt-precorrin-5B (C(1))-methyltransferase CbiD, at 1,189,053 to 1,190,150
_RS06195
MMP_RS06200: MMP_RS06200 - M24 family metallopeptidase, at 1,190,190 to 1,191,209
_RS06200
Position (kb)
1189
1190
1191 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1188.089 kb on + strand at 1188.094 kb on + strand at 1188.185 kb on + strand, within MMP_RS06185 at 1188.373 kb on + strand, within MMP_RS06185 at 1188.373 kb on + strand, within MMP_RS06185 at 1188.403 kb on - strand, within MMP_RS06185 at 1188.404 kb on + strand, within MMP_RS06185 at 1188.557 kb on - strand, within MMP_RS06185 at 1188.576 kb on - strand, within MMP_RS06185 at 1188.580 kb on - strand, within MMP_RS06185 at 1188.580 kb on - strand, within MMP_RS06185 at 1188.598 kb on - strand at 1188.598 kb on - strand at 1188.599 kb on + strand at 1188.653 kb on + strand at 1188.655 kb on - strand at 1188.739 kb on + strand, within MMP_RS06190 at 1188.739 kb on + strand, within MMP_RS06190 at 1188.739 kb on + strand, within MMP_RS06190 at 1188.740 kb on - strand, within MMP_RS06190 at 1188.740 kb on - strand, within MMP_RS06190 at 1188.741 kb on + strand, within MMP_RS06190 at 1188.777 kb on - strand, within MMP_RS06190 at 1188.786 kb on + strand, within MMP_RS06190 at 1188.788 kb on + strand, within MMP_RS06190 at 1189.071 kb on - strand at 1189.072 kb on + strand at 1189.074 kb on + strand at 1189.085 kb on - strand at 1189.090 kb on + strand at 1189.094 kb on - strand at 1189.095 kb on + strand at 1189.095 kb on + strand at 1189.866 kb on + strand at 1190.152 kb on - strand at 1190.153 kb on + strand at 1190.191 kb on - strand at 1190.327 kb on - strand, within MMP_RS06200 at 1190.327 kb on - strand, within MMP_RS06200 at 1190.403 kb on - strand, within MMP_RS06200 at 1190.404 kb on + strand, within MMP_RS06200 at 1190.429 kb on - strand, within MMP_RS06200 at 1190.520 kb on + strand, within MMP_RS06200 at 1190.573 kb on - strand, within MMP_RS06200 at 1190.708 kb on - strand, within MMP_RS06200 at 1190.789 kb on - strand, within MMP_RS06200 at 1190.790 kb on + strand, within MMP_RS06200 at 1190.793 kb on + strand, within MMP_RS06200 at 1190.797 kb on - strand, within MMP_RS06200 at 1190.797 kb on - strand, within MMP_RS06200 at 1190.798 kb on + strand, within MMP_RS06200 at 1190.895 kb on - strand, within MMP_RS06200 at 1190.970 kb on + strand, within MMP_RS06200 at 1191.017 kb on + strand, within MMP_RS06200 at 1191.055 kb on + strand, within MMP_RS06200 at 1191.087 kb on + strand, within MMP_RS06200 at 1191.129 kb on - strand at 1191.129 kb on - strand at 1191.149 kb on - strand at 1191.149 kb on - strand at 1191.150 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 1,188,089 + -0.1 1,188,094 + +0.2 1,188,185 + MMP_RS06185 0.16 +1.0 1,188,373 + MMP_RS06185 0.50 +1.2 1,188,373 + MMP_RS06185 0.50 +0.3 1,188,403 - MMP_RS06185 0.55 +0.2 1,188,404 + MMP_RS06185 0.55 +0.1 1,188,557 - MMP_RS06185 0.83 +0.3 1,188,576 - MMP_RS06185 0.86 -0.5 1,188,580 - MMP_RS06185 0.87 -0.1 1,188,580 - MMP_RS06185 0.87 -0.3 1,188,598 - +0.9 1,188,598 - -1.0 1,188,599 + -0.0 1,188,653 + -0.2 1,188,655 - +1.1 1,188,739 + MMP_RS06190 0.20 +0.1 1,188,739 + MMP_RS06190 0.20 -0.1 1,188,739 + MMP_RS06190 0.20 +0.1 1,188,740 - MMP_RS06190 0.20 +0.7 1,188,740 - MMP_RS06190 0.20 +0.7 1,188,741 + MMP_RS06190 0.21 -0.1 1,188,777 - MMP_RS06190 0.30 +0.7 1,188,786 + MMP_RS06190 0.32 +1.0 1,188,788 + MMP_RS06190 0.33 -0.2 1,189,071 - -1.4 1,189,072 + -1.2 1,189,074 + -4.7 1,189,085 - -1.5 1,189,090 + -0.7 1,189,094 - -2.6 1,189,095 + -4.7 1,189,095 + -3.5 1,189,866 + -3.7 1,190,152 - -3.2 1,190,153 + -3.1 1,190,191 - +0.6 1,190,327 - MMP_RS06200 0.13 -0.4 1,190,327 - MMP_RS06200 0.13 -0.3 1,190,403 - MMP_RS06200 0.21 +0.2 1,190,404 + MMP_RS06200 0.21 +0.7 1,190,429 - MMP_RS06200 0.23 -3.1 1,190,520 + MMP_RS06200 0.32 +0.3 1,190,573 - MMP_RS06200 0.38 -1.0 1,190,708 - MMP_RS06200 0.51 -0.2 1,190,789 - MMP_RS06200 0.59 -0.5 1,190,790 + MMP_RS06200 0.59 -0.7 1,190,793 + MMP_RS06200 0.59 +0.4 1,190,797 - MMP_RS06200 0.60 -0.6 1,190,797 - MMP_RS06200 0.60 +0.2 1,190,798 + MMP_RS06200 0.60 +0.5 1,190,895 - MMP_RS06200 0.69 -1.0 1,190,970 + MMP_RS06200 0.76 -0.5 1,191,017 + MMP_RS06200 0.81 +0.4 1,191,055 + MMP_RS06200 0.85 +0.4 1,191,087 + MMP_RS06200 0.88 +1.1 1,191,129 - -0.2 1,191,129 - +0.2 1,191,149 - -0.0 1,191,149 - -0.4 1,191,150 + -0.1
Or see this region's nucleotide sequence