Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06175 and MMP_RS06180 are separated by 135 nucleotides MMP_RS06180 and MMP_RS06185 are separated by 250 nucleotides MMP_RS06185 and MMP_RS06190 are separated by 9 nucleotides
MMP_RS06175: MMP_RS06175 - winged helix-turn-helix transcriptional regulator, at 1,185,544 to 1,186,398
_RS06175
MMP_RS06180: MMP_RS06180 - diaminopimelate decarboxylase, at 1,186,534 to 1,187,844
_RS06180
MMP_RS06185: MMP_RS06185 - thermonuclease family protein, at 1,188,095 to 1,188,652
_RS06185
MMP_RS06190: MMP_RS06190 - tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO, at 1,188,662 to 1,189,045
_RS06190
Position (kb)
1186
1187
1188 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1185.585 kb on - strand at 1185.746 kb on + strand, within MMP_RS06175 at 1185.822 kb on + strand, within MMP_RS06175 at 1185.857 kb on - strand, within MMP_RS06175 at 1185.858 kb on + strand, within MMP_RS06175 at 1185.876 kb on - strand, within MMP_RS06175 at 1185.889 kb on - strand, within MMP_RS06175 at 1185.918 kb on + strand, within MMP_RS06175 at 1186.206 kb on - strand, within MMP_RS06175 at 1186.305 kb on - strand, within MMP_RS06175 at 1186.379 kb on + strand at 1186.401 kb on + strand at 1186.443 kb on + strand at 1186.446 kb on + strand at 1186.449 kb on - strand at 1186.461 kb on - strand at 1186.462 kb on + strand at 1186.462 kb on + strand at 1186.462 kb on + strand at 1186.462 kb on + strand at 1186.463 kb on - strand at 1186.464 kb on + strand at 1186.465 kb on - strand at 1186.465 kb on - strand at 1186.466 kb on + strand at 1186.466 kb on + strand at 1186.483 kb on - strand at 1186.483 kb on - strand at 1186.487 kb on - strand at 1186.498 kb on + strand at 1186.614 kb on - strand at 1187.302 kb on - strand at 1187.361 kb on + strand at 1187.537 kb on + strand at 1187.722 kb on - strand at 1187.782 kb on + strand at 1187.820 kb on - strand at 1187.848 kb on + strand at 1187.878 kb on - strand at 1187.884 kb on - strand at 1187.903 kb on - strand at 1187.908 kb on - strand at 1187.918 kb on + strand at 1187.956 kb on - strand at 1187.959 kb on - strand at 1187.960 kb on + strand at 1188.089 kb on + strand at 1188.094 kb on + strand at 1188.185 kb on + strand, within MMP_RS06185 at 1188.373 kb on + strand, within MMP_RS06185 at 1188.373 kb on + strand, within MMP_RS06185 at 1188.403 kb on - strand, within MMP_RS06185 at 1188.404 kb on + strand, within MMP_RS06185 at 1188.557 kb on - strand, within MMP_RS06185 at 1188.576 kb on - strand, within MMP_RS06185 at 1188.580 kb on - strand, within MMP_RS06185 at 1188.580 kb on - strand, within MMP_RS06185 at 1188.598 kb on - strand at 1188.598 kb on - strand at 1188.599 kb on + strand at 1188.653 kb on + strand at 1188.655 kb on - strand at 1188.739 kb on + strand, within MMP_RS06190 at 1188.739 kb on + strand, within MMP_RS06190 at 1188.739 kb on + strand, within MMP_RS06190 at 1188.740 kb on - strand, within MMP_RS06190 at 1188.740 kb on - strand, within MMP_RS06190 at 1188.741 kb on + strand, within MMP_RS06190 at 1188.777 kb on - strand, within MMP_RS06190 at 1188.786 kb on + strand, within MMP_RS06190 at 1188.788 kb on + strand, within MMP_RS06190
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 1,185,585 - -3.1 1,185,746 + MMP_RS06175 0.24 -3.9 1,185,822 + MMP_RS06175 0.33 -1.4 1,185,857 - MMP_RS06175 0.37 -1.2 1,185,858 + MMP_RS06175 0.37 -2.0 1,185,876 - MMP_RS06175 0.39 -1.8 1,185,889 - MMP_RS06175 0.40 -2.5 1,185,918 + MMP_RS06175 0.44 -1.5 1,186,206 - MMP_RS06175 0.77 -1.4 1,186,305 - MMP_RS06175 0.89 -1.5 1,186,379 + +0.4 1,186,401 + +0.1 1,186,443 + -1.5 1,186,446 + +0.1 1,186,449 - +0.9 1,186,461 - -0.3 1,186,462 + +0.2 1,186,462 + +0.7 1,186,462 + -0.1 1,186,462 + -0.2 1,186,463 - -0.3 1,186,464 + +1.3 1,186,465 - +0.5 1,186,465 - -0.3 1,186,466 + -0.2 1,186,466 + +0.2 1,186,483 - -1.5 1,186,483 - -0.7 1,186,487 - -0.4 1,186,498 + -1.6 1,186,614 - -3.7 1,187,302 - -3.5 1,187,361 + -2.0 1,187,537 + -4.0 1,187,722 - -3.1 1,187,782 + -1.3 1,187,820 - -3.5 1,187,848 + +0.1 1,187,878 - +0.4 1,187,884 - -0.2 1,187,903 - +0.5 1,187,908 - +0.0 1,187,918 + -0.7 1,187,956 - +0.9 1,187,959 - +0.4 1,187,960 + +1.0 1,188,089 + -0.1 1,188,094 + +0.2 1,188,185 + MMP_RS06185 0.16 +1.0 1,188,373 + MMP_RS06185 0.50 +1.2 1,188,373 + MMP_RS06185 0.50 +0.3 1,188,403 - MMP_RS06185 0.55 +0.2 1,188,404 + MMP_RS06185 0.55 +0.1 1,188,557 - MMP_RS06185 0.83 +0.3 1,188,576 - MMP_RS06185 0.86 -0.5 1,188,580 - MMP_RS06185 0.87 -0.1 1,188,580 - MMP_RS06185 0.87 -0.3 1,188,598 - +0.9 1,188,598 - -1.0 1,188,599 + -0.0 1,188,653 + -0.2 1,188,655 - +1.1 1,188,739 + MMP_RS06190 0.20 +0.1 1,188,739 + MMP_RS06190 0.20 -0.1 1,188,739 + MMP_RS06190 0.20 +0.1 1,188,740 - MMP_RS06190 0.20 +0.7 1,188,740 - MMP_RS06190 0.20 +0.7 1,188,741 + MMP_RS06190 0.21 -0.1 1,188,777 - MMP_RS06190 0.30 +0.7 1,188,786 + MMP_RS06190 0.32 +1.0 1,188,788 + MMP_RS06190 0.33 -0.2
Or see this region's nucleotide sequence