Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05875 and MMP_RS05880 overlap by 11 nucleotides MMP_RS05880 and MMP_RS05885 are separated by 175 nucleotides MMP_RS05885 and MMP_RS05890 are separated by 263 nucleotides
MMP_RS05875: MMP_RS05875 - hydroxyethylthiazole kinase, at 1,128,064 to 1,128,864
_RS05875
MMP_RS05880: MMP_RS05880 - thiamine phosphate synthase, at 1,128,854 to 1,129,477
_RS05880
MMP_RS05885: MMP_RS05885 - ferredoxin family protein, at 1,129,653 to 1,129,898
_RS05885
MMP_RS05890: MMP_RS05890 - ATP-dependent helicase, at 1,130,162 to 1,132,729
_RS05890
Position (kb)
1129
1130 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1128.658 kb on + strand, within MMP_RS05875 at 1129.275 kb on - strand at 1129.470 kb on - strand at 1129.514 kb on + strand at 1129.600 kb on + strand at 1129.606 kb on - strand at 1129.607 kb on + strand at 1129.660 kb on - strand at 1129.934 kb on - strand at 1129.937 kb on + strand at 1129.937 kb on + strand at 1129.942 kb on - strand at 1129.986 kb on + strand at 1130.030 kb on + strand at 1130.066 kb on + strand at 1130.191 kb on - strand at 1130.195 kb on + strand at 1130.311 kb on - strand at 1130.364 kb on + strand at 1130.373 kb on + strand at 1130.374 kb on - strand at 1130.374 kb on - strand at 1130.375 kb on + strand at 1130.507 kb on + strand, within MMP_RS05890 at 1130.668 kb on - strand, within MMP_RS05890 at 1130.689 kb on - strand, within MMP_RS05890 at 1130.837 kb on + strand, within MMP_RS05890 at 1130.892 kb on - strand, within MMP_RS05890
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 1,128,658 + MMP_RS05875 0.74 +0.7 1,129,275 - -4.9 1,129,470 - -2.8 1,129,514 + +1.3 1,129,600 + +0.0 1,129,606 - -0.4 1,129,607 + +0.2 1,129,660 - -0.6 1,129,934 - -0.6 1,129,937 + +0.5 1,129,937 + +0.5 1,129,942 - +0.6 1,129,986 + +0.6 1,130,030 + -0.1 1,130,066 + +0.5 1,130,191 - +0.2 1,130,195 + -0.3 1,130,311 - +2.1 1,130,364 + +0.1 1,130,373 + -0.1 1,130,374 - +0.3 1,130,374 - +0.3 1,130,375 + -0.2 1,130,507 + MMP_RS05890 0.13 +0.6 1,130,668 - MMP_RS05890 0.20 +0.3 1,130,689 - MMP_RS05890 0.21 +0.2 1,130,837 + MMP_RS05890 0.26 -0.4 1,130,892 - MMP_RS05890 0.28 +0.4
Or see this region's nucleotide sequence