Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05750 and MMP_RS05755 are separated by 56 nucleotides MMP_RS05755 and MMP_RS05760 are separated by 13 nucleotides MMP_RS05760 and MMP_RS05765 are separated by 338 nucleotides
MMP_RS05750: MMP_RS05750 - transketolase family protein, at 1,103,223 to 1,104,161
_RS05750
MMP_RS05755: MMP_RS05755 - ribulose-phosphate 3-epimerase, at 1,104,218 to 1,104,877
_RS05755
MMP_RS05760: MMP_RS05760 - transketolase, at 1,104,891 to 1,105,718
_RS05760
MMP_RS05765: MMP_RS05765 - M42 family metallopeptidase, at 1,106,057 to 1,107,109
_RS05765
Position (kb)
1104
1105
1106 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1104.081 kb on - strand at 1104.162 kb on + strand at 1104.163 kb on - strand at 1104.597 kb on - strand at 1104.708 kb on + strand at 1104.746 kb on - strand at 1104.991 kb on + strand at 1105.093 kb on + strand at 1105.394 kb on - strand at 1105.854 kb on - strand at 1105.867 kb on + strand at 1105.869 kb on - strand at 1105.869 kb on - strand at 1105.891 kb on - strand at 1105.992 kb on - strand at 1106.008 kb on - strand at 1106.008 kb on - strand at 1106.009 kb on + strand at 1106.009 kb on + strand at 1106.043 kb on + strand at 1106.125 kb on + strand at 1106.274 kb on - strand, within MMP_RS05765 at 1106.476 kb on - strand, within MMP_RS05765
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 1,104,081 - -4.2 1,104,162 + -0.1 1,104,163 - -0.6 1,104,597 - -2.7 1,104,708 + -2.0 1,104,746 - -1.5 1,104,991 + -0.6 1,105,093 + -3.3 1,105,394 - -2.8 1,105,854 - -0.8 1,105,867 + -1.4 1,105,869 - +0.9 1,105,869 - +0.0 1,105,891 - -0.6 1,105,992 - -0.4 1,106,008 - -0.0 1,106,008 - -0.6 1,106,009 + -0.4 1,106,009 + +0.6 1,106,043 + -0.3 1,106,125 + -0.5 1,106,274 - MMP_RS05765 0.21 -1.5 1,106,476 - MMP_RS05765 0.40 -0.8
Or see this region's nucleotide sequence