Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05740 and MMP_RS05745 are separated by 4 nucleotides MMP_RS05745 and MMP_RS05750 are separated by 60 nucleotides MMP_RS05750 and MMP_RS05755 are separated by 56 nucleotides MMP_RS05755 and MMP_RS05760 are separated by 13 nucleotides
MMP_RS05740: MMP_RS05740 - oligosaccharide repeat unit polymerase family protein, at 1,101,329 to 1,102,402
_RS05740
MMP_RS05745: MMP_RS05745 - hypothetical protein, at 1,102,407 to 1,103,162
_RS05745
MMP_RS05750: MMP_RS05750 - transketolase family protein, at 1,103,223 to 1,104,161
_RS05750
MMP_RS05755: MMP_RS05755 - ribulose-phosphate 3-epimerase, at 1,104,218 to 1,104,877
_RS05755
MMP_RS05760: MMP_RS05760 - transketolase, at 1,104,891 to 1,105,718
_RS05760
Position (kb)
1103
1104
1105 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1102.576 kb on + strand at 1102.739 kb on - strand at 1102.740 kb on + strand at 1102.740 kb on + strand at 1102.750 kb on + strand at 1102.787 kb on - strand at 1102.830 kb on + strand at 1102.932 kb on - strand at 1103.115 kb on - strand at 1103.195 kb on - strand at 1103.207 kb on + strand at 1103.207 kb on + strand at 1103.537 kb on - strand at 1103.676 kb on - strand at 1104.081 kb on - strand at 1104.162 kb on + strand at 1104.163 kb on - strand at 1104.597 kb on - strand at 1104.708 kb on + strand at 1104.746 kb on - strand at 1104.991 kb on + strand at 1105.093 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 1,102,576 + -1.7 1,102,739 - -2.7 1,102,740 + -0.9 1,102,740 + +0.6 1,102,750 + -1.9 1,102,787 - -2.5 1,102,830 + -1.1 1,102,932 - -2.6 1,103,115 - -4.4 1,103,195 - -0.7 1,103,207 + -1.1 1,103,207 + -0.0 1,103,537 - -2.1 1,103,676 - -1.9 1,104,081 - -4.2 1,104,162 + -0.1 1,104,163 - -0.6 1,104,597 - -2.7 1,104,708 + -2.0 1,104,746 - -1.5 1,104,991 + -0.6 1,105,093 + -3.3
Or see this region's nucleotide sequence