Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05700 and MMP_RS05705 are separated by 49 nucleotides MMP_RS05705 and MMP_RS05710 are separated by 57 nucleotides MMP_RS05710 and MMP_RS05715 are separated by 12 nucleotides MMP_RS05715 and MMP_RS05720 are separated by 0 nucleotides MMP_RS05720 and MMP_RS05725 are separated by 13 nucleotides
MMP_RS05700: MMP_RS05700 - TfuA-related McrA-glycine thioamidation protein, at 1,095,173 to 1,095,817
_RS05700
MMP_RS05705: MMP_RS05705 - aspartate carbamoyltransferase regulatory subunit, at 1,095,867 to 1,096,313
_RS05705
MMP_RS05710: MMP_RS05710 - ADP-forming succinate--CoA ligase subunit beta, at 1,096,371 to 1,097,459
_RS05710
MMP_RS05715: MMP_RS05715 - hypothetical protein, at 1,097,472 to 1,097,783
_RS05715
MMP_RS05720: MMP_RS05720 - signal recognition particle subunit SRP19/SEC65 family protein, at 1,097,784 to 1,098,053
_RS05720
MMP_RS05725: MMP_RS05725 - magnesium/cobalt transporter CorA, at 1,098,067 to 1,099,017
_RS05725
Position (kb)
1096
1097
1098 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1095.385 kb on - strand, within MMP_RS05700 at 1095.396 kb on - strand, within MMP_RS05700 at 1095.475 kb on - strand, within MMP_RS05700 at 1095.476 kb on + strand, within MMP_RS05700 at 1095.485 kb on + strand, within MMP_RS05700 at 1095.524 kb on + strand, within MMP_RS05700 at 1095.667 kb on - strand, within MMP_RS05700 at 1095.668 kb on + strand, within MMP_RS05700 at 1095.801 kb on + strand at 1095.846 kb on - strand at 1095.846 kb on - strand at 1096.070 kb on + strand at 1096.115 kb on + strand at 1096.115 kb on + strand at 1096.301 kb on + strand at 1096.483 kb on - strand at 1096.653 kb on - strand at 1096.736 kb on + strand at 1096.801 kb on + strand at 1096.995 kb on - strand at 1097.330 kb on - strand at 1097.453 kb on - strand at 1097.529 kb on + strand, within MMP_RS05715 at 1097.541 kb on - strand, within MMP_RS05715 at 1097.542 kb on + strand, within MMP_RS05715 at 1097.544 kb on + strand, within MMP_RS05715 at 1097.562 kb on - strand, within MMP_RS05715 at 1097.562 kb on - strand, within MMP_RS05715 at 1097.563 kb on + strand, within MMP_RS05715 at 1097.563 kb on + strand, within MMP_RS05715 at 1097.584 kb on + strand, within MMP_RS05715 at 1097.603 kb on + strand, within MMP_RS05715 at 1097.653 kb on + strand, within MMP_RS05715 at 1097.799 kb on + strand at 1097.799 kb on + strand at 1097.802 kb on - strand at 1097.802 kb on - strand at 1097.802 kb on - strand at 1097.802 kb on - strand at 1097.855 kb on - strand, within MMP_RS05720 at 1097.910 kb on + strand, within MMP_RS05720 at 1097.912 kb on + strand, within MMP_RS05720 at 1098.024 kb on + strand, within MMP_RS05720 at 1098.106 kb on - strand at 1098.176 kb on - strand at 1098.195 kb on + strand at 1098.343 kb on - strand at 1098.441 kb on - strand at 1098.450 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 1,095,385 - MMP_RS05700 0.33 -1.8 1,095,396 - MMP_RS05700 0.35 -0.3 1,095,475 - MMP_RS05700 0.47 -0.3 1,095,476 + MMP_RS05700 0.47 -1.0 1,095,485 + MMP_RS05700 0.48 -0.5 1,095,524 + MMP_RS05700 0.54 -2.5 1,095,667 - MMP_RS05700 0.77 +0.8 1,095,668 + MMP_RS05700 0.77 +0.4 1,095,801 + +0.7 1,095,846 - -1.6 1,095,846 - -4.3 1,096,070 + -1.7 1,096,115 + -1.5 1,096,115 + -1.8 1,096,301 + -0.5 1,096,483 - -0.1 1,096,653 - -2.3 1,096,736 + -2.9 1,096,801 + -3.2 1,096,995 - -0.6 1,097,330 - -4.5 1,097,453 - +0.1 1,097,529 + MMP_RS05715 0.18 -0.1 1,097,541 - MMP_RS05715 0.22 +0.3 1,097,542 + MMP_RS05715 0.22 -0.1 1,097,544 + MMP_RS05715 0.23 +0.7 1,097,562 - MMP_RS05715 0.29 +1.1 1,097,562 - MMP_RS05715 0.29 +1.5 1,097,563 + MMP_RS05715 0.29 +0.6 1,097,563 + MMP_RS05715 0.29 +0.5 1,097,584 + MMP_RS05715 0.36 -0.6 1,097,603 + MMP_RS05715 0.42 +0.2 1,097,653 + MMP_RS05715 0.58 +1.0 1,097,799 + +0.2 1,097,799 + +0.2 1,097,802 - -0.3 1,097,802 - +1.1 1,097,802 - -1.0 1,097,802 - -0.1 1,097,855 - MMP_RS05720 0.26 +0.3 1,097,910 + MMP_RS05720 0.47 +2.1 1,097,912 + MMP_RS05720 0.47 +0.0 1,098,024 + MMP_RS05720 0.89 +0.1 1,098,106 - -1.9 1,098,176 - -2.6 1,098,195 + -4.5 1,098,343 - -0.6 1,098,441 - -3.8 1,098,450 - -3.8
Or see this region's nucleotide sequence