Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05680 and MMP_RS05685 overlap by 4 nucleotides MMP_RS05685 and MMP_RS05690 are separated by 216 nucleotides MMP_RS05690 and MMP_RS05695 are separated by 156 nucleotides MMP_RS05695 and MMP_RS05700 overlap by 4 nucleotides
MMP_RS05680: MMP_RS05680 - phosphate signaling complex protein PhoU, at 1,091,935 to 1,092,636
_RS05680
MMP_RS05685: MMP_RS05685 - winged helix-turn-helix domain-containing protein, at 1,092,633 to 1,093,040
_RS05685
MMP_RS05690: MMP_RS05690 - acetylornithine transaminase, at 1,093,257 to 1,094,444
_RS05690
MMP_RS05695: MMP_RS05695 - phospholipase D-like domain-containing protein, at 1,094,601 to 1,095,176
_RS05695
MMP_RS05700: MMP_RS05700 - TfuA-related McrA-glycine thioamidation protein, at 1,095,173 to 1,095,817
_RS05700
Position (kb)
1093
1094
1095 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1092.291 kb on - strand, within MMP_RS05680 at 1092.385 kb on - strand, within MMP_RS05680 at 1092.397 kb on + strand, within MMP_RS05680 at 1092.414 kb on + strand, within MMP_RS05680 at 1092.414 kb on + strand, within MMP_RS05680 at 1092.416 kb on + strand, within MMP_RS05680 at 1092.445 kb on + strand, within MMP_RS05680 at 1092.505 kb on - strand, within MMP_RS05680 at 1092.505 kb on - strand, within MMP_RS05680 at 1092.506 kb on + strand, within MMP_RS05680 at 1092.546 kb on - strand, within MMP_RS05680 at 1092.547 kb on + strand, within MMP_RS05680 at 1092.558 kb on - strand, within MMP_RS05680 at 1092.602 kb on - strand at 1092.644 kb on + strand at 1092.686 kb on - strand, within MMP_RS05685 at 1092.857 kb on - strand, within MMP_RS05685 at 1092.859 kb on - strand, within MMP_RS05685 at 1092.859 kb on - strand, within MMP_RS05685 at 1092.859 kb on - strand, within MMP_RS05685 at 1092.860 kb on + strand, within MMP_RS05685 at 1092.860 kb on + strand, within MMP_RS05685 at 1092.953 kb on + strand, within MMP_RS05685 at 1092.954 kb on - strand, within MMP_RS05685 at 1092.958 kb on - strand, within MMP_RS05685 at 1092.987 kb on - strand, within MMP_RS05685 at 1093.061 kb on - strand at 1093.063 kb on - strand at 1093.064 kb on + strand at 1093.087 kb on + strand at 1093.100 kb on + strand at 1093.100 kb on + strand at 1093.131 kb on + strand at 1093.131 kb on + strand at 1093.133 kb on + strand at 1093.147 kb on - strand at 1093.147 kb on - strand at 1093.148 kb on + strand at 1093.148 kb on + strand at 1093.179 kb on - strand at 1093.302 kb on - strand at 1093.302 kb on - strand at 1093.549 kb on + strand at 1094.003 kb on - strand at 1094.081 kb on + strand at 1094.359 kb on + strand at 1094.384 kb on + strand at 1094.433 kb on - strand at 1094.507 kb on - strand at 1094.510 kb on - strand at 1094.510 kb on - strand at 1094.528 kb on - strand at 1094.531 kb on + strand at 1094.643 kb on + strand at 1094.673 kb on - strand, within MMP_RS05695 at 1094.678 kb on + strand, within MMP_RS05695 at 1094.779 kb on + strand, within MMP_RS05695 at 1094.828 kb on - strand, within MMP_RS05695 at 1094.842 kb on - strand, within MMP_RS05695 at 1094.844 kb on - strand, within MMP_RS05695 at 1094.844 kb on - strand, within MMP_RS05695 at 1094.870 kb on + strand, within MMP_RS05695 at 1095.017 kb on - strand, within MMP_RS05695 at 1095.022 kb on - strand, within MMP_RS05695 at 1095.028 kb on - strand, within MMP_RS05695 at 1095.029 kb on + strand, within MMP_RS05695 at 1095.252 kb on - strand, within MMP_RS05700 at 1095.253 kb on + strand, within MMP_RS05700 at 1095.253 kb on + strand, within MMP_RS05700 at 1095.279 kb on - strand, within MMP_RS05700 at 1095.302 kb on - strand, within MMP_RS05700 at 1095.309 kb on - strand, within MMP_RS05700 at 1095.309 kb on - strand, within MMP_RS05700 at 1095.318 kb on - strand, within MMP_RS05700 at 1095.385 kb on - strand, within MMP_RS05700 at 1095.396 kb on - strand, within MMP_RS05700
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 1,092,291 - MMP_RS05680 0.51 -1.1 1,092,385 - MMP_RS05680 0.64 -0.2 1,092,397 + MMP_RS05680 0.66 +0.1 1,092,414 + MMP_RS05680 0.68 -0.1 1,092,414 + MMP_RS05680 0.68 -0.7 1,092,416 + MMP_RS05680 0.69 +1.1 1,092,445 + MMP_RS05680 0.73 +0.1 1,092,505 - MMP_RS05680 0.81 -0.3 1,092,505 - MMP_RS05680 0.81 -0.2 1,092,506 + MMP_RS05680 0.81 +0.4 1,092,546 - MMP_RS05680 0.87 -0.2 1,092,547 + MMP_RS05680 0.87 +1.0 1,092,558 - MMP_RS05680 0.89 -0.7 1,092,602 - -1.3 1,092,644 + +0.5 1,092,686 - MMP_RS05685 0.13 +0.3 1,092,857 - MMP_RS05685 0.55 +1.0 1,092,859 - MMP_RS05685 0.55 -0.2 1,092,859 - MMP_RS05685 0.55 -0.7 1,092,859 - MMP_RS05685 0.55 -0.4 1,092,860 + MMP_RS05685 0.56 -1.4 1,092,860 + MMP_RS05685 0.56 +0.0 1,092,953 + MMP_RS05685 0.78 -0.5 1,092,954 - MMP_RS05685 0.79 +0.7 1,092,958 - MMP_RS05685 0.80 +0.3 1,092,987 - MMP_RS05685 0.87 -0.9 1,093,061 - -0.6 1,093,063 - -0.1 1,093,064 + +3.7 1,093,087 + +0.1 1,093,100 + -0.4 1,093,100 + -0.8 1,093,131 + -0.6 1,093,131 + -0.4 1,093,133 + +0.7 1,093,147 - +0.1 1,093,147 - +0.3 1,093,148 + -0.1 1,093,148 + +0.2 1,093,179 - +0.8 1,093,302 - -2.6 1,093,302 - -3.3 1,093,549 + -3.1 1,094,003 - -2.1 1,094,081 + -3.9 1,094,359 + -1.7 1,094,384 + -2.2 1,094,433 - -2.5 1,094,507 - +0.9 1,094,510 - +0.3 1,094,510 - +0.4 1,094,528 - +1.6 1,094,531 + +0.7 1,094,643 + +0.8 1,094,673 - MMP_RS05695 0.12 +0.8 1,094,678 + MMP_RS05695 0.13 +0.0 1,094,779 + MMP_RS05695 0.31 +1.4 1,094,828 - MMP_RS05695 0.39 +1.3 1,094,842 - MMP_RS05695 0.42 +0.5 1,094,844 - MMP_RS05695 0.42 +1.0 1,094,844 - MMP_RS05695 0.42 -0.1 1,094,870 + MMP_RS05695 0.47 +1.3 1,095,017 - MMP_RS05695 0.72 +1.6 1,095,022 - MMP_RS05695 0.73 +0.1 1,095,028 - MMP_RS05695 0.74 +1.1 1,095,029 + MMP_RS05695 0.74 +0.3 1,095,252 - MMP_RS05700 0.12 -0.0 1,095,253 + MMP_RS05700 0.12 -0.3 1,095,253 + MMP_RS05700 0.12 -2.2 1,095,279 - MMP_RS05700 0.16 -3.3 1,095,302 - MMP_RS05700 0.20 +0.8 1,095,309 - MMP_RS05700 0.21 +0.1 1,095,309 - MMP_RS05700 0.21 +0.1 1,095,318 - MMP_RS05700 0.22 -0.1 1,095,385 - MMP_RS05700 0.33 -1.8 1,095,396 - MMP_RS05700 0.35 -0.3
Or see this region's nucleotide sequence