Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05625 and MMP_RS05630 are separated by 176 nucleotides MMP_RS05630 and MMP_RS05635 are separated by 30 nucleotides
MMP_RS05625: MMP_RS05625 - glycosyltransferase, at 1,079,621 to 1,080,823
_RS05625
MMP_RS05630: MMP_RS05630 - flippase, at 1,081,000 to 1,082,502
_RS05630
MMP_RS05635: MMP_RS05635 - NAD-dependent epimerase/dehydratase family protein, at 1,082,533 to 1,083,411
_RS05635
Position (kb)
1080
1081
1082
1083 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1080.027 kb on - strand, within MMP_RS05625 at 1080.087 kb on - strand, within MMP_RS05625 at 1080.101 kb on + strand, within MMP_RS05625 at 1080.141 kb on - strand, within MMP_RS05625 at 1080.280 kb on + strand, within MMP_RS05625 at 1080.303 kb on - strand, within MMP_RS05625 at 1080.303 kb on - strand, within MMP_RS05625 at 1080.306 kb on - strand, within MMP_RS05625 at 1080.309 kb on - strand, within MMP_RS05625 at 1080.309 kb on - strand, within MMP_RS05625 at 1080.309 kb on - strand, within MMP_RS05625 at 1080.310 kb on + strand, within MMP_RS05625 at 1080.310 kb on + strand, within MMP_RS05625 at 1080.325 kb on + strand, within MMP_RS05625 at 1080.345 kb on - strand, within MMP_RS05625 at 1080.346 kb on + strand, within MMP_RS05625 at 1080.350 kb on + strand, within MMP_RS05625 at 1080.351 kb on - strand, within MMP_RS05625 at 1080.351 kb on - strand, within MMP_RS05625 at 1080.352 kb on + strand, within MMP_RS05625 at 1080.352 kb on + strand, within MMP_RS05625 at 1080.356 kb on + strand, within MMP_RS05625 at 1080.372 kb on - strand, within MMP_RS05625 at 1080.399 kb on - strand, within MMP_RS05625 at 1080.399 kb on - strand, within MMP_RS05625 at 1080.399 kb on - strand, within MMP_RS05625 at 1080.400 kb on + strand, within MMP_RS05625 at 1080.401 kb on - strand, within MMP_RS05625 at 1080.401 kb on - strand, within MMP_RS05625 at 1080.402 kb on + strand, within MMP_RS05625 at 1080.405 kb on - strand, within MMP_RS05625 at 1080.409 kb on + strand, within MMP_RS05625 at 1080.438 kb on - strand, within MMP_RS05625 at 1080.475 kb on - strand, within MMP_RS05625 at 1080.552 kb on + strand, within MMP_RS05625 at 1080.588 kb on - strand, within MMP_RS05625 at 1080.589 kb on + strand, within MMP_RS05625 at 1080.589 kb on + strand, within MMP_RS05625 at 1080.616 kb on - strand, within MMP_RS05625 at 1080.616 kb on - strand, within MMP_RS05625 at 1080.617 kb on + strand, within MMP_RS05625 at 1080.621 kb on - strand, within MMP_RS05625 at 1080.622 kb on + strand, within MMP_RS05625 at 1080.622 kb on + strand, within MMP_RS05625 at 1080.663 kb on - strand, within MMP_RS05625 at 1080.667 kb on + strand, within MMP_RS05625 at 1080.668 kb on - strand, within MMP_RS05625 at 1080.670 kb on - strand, within MMP_RS05625 at 1080.671 kb on + strand, within MMP_RS05625 at 1080.691 kb on - strand, within MMP_RS05625 at 1080.692 kb on + strand, within MMP_RS05625 at 1080.692 kb on + strand, within MMP_RS05625 at 1080.692 kb on + strand, within MMP_RS05625 at 1080.692 kb on + strand, within MMP_RS05625 at 1080.692 kb on + strand, within MMP_RS05625 at 1080.693 kb on - strand, within MMP_RS05625 at 1080.693 kb on - strand, within MMP_RS05625 at 1080.694 kb on + strand, within MMP_RS05625 at 1080.694 kb on + strand, within MMP_RS05625 at 1080.694 kb on + strand, within MMP_RS05625 at 1080.694 kb on + strand, within MMP_RS05625 at 1080.705 kb on - strand at 1080.751 kb on - strand at 1080.756 kb on + strand at 1080.770 kb on + strand at 1080.804 kb on - strand at 1080.804 kb on - strand at 1080.804 kb on - strand at 1080.804 kb on - strand at 1080.805 kb on + strand at 1080.805 kb on + strand at 1080.805 kb on + strand at 1080.805 kb on + strand at 1080.806 kb on - strand at 1080.806 kb on - strand at 1080.806 kb on - strand at 1080.806 kb on - strand at 1080.807 kb on + strand at 1080.807 kb on + strand at 1080.807 kb on + strand at 1080.807 kb on + strand at 1080.807 kb on + strand at 1080.808 kb on - strand at 1080.808 kb on - strand at 1080.809 kb on + strand at 1080.809 kb on + strand at 1080.809 kb on + strand at 1080.858 kb on - strand at 1080.858 kb on - strand at 1080.859 kb on + strand at 1080.860 kb on - strand at 1080.864 kb on + strand at 1080.876 kb on - strand at 1080.877 kb on + strand at 1080.901 kb on - strand at 1080.908 kb on + strand at 1080.913 kb on + strand at 1080.959 kb on + strand at 1080.959 kb on + strand at 1080.979 kb on - strand at 1080.985 kb on + strand at 1081.001 kb on - strand at 1081.031 kb on + strand at 1081.106 kb on + strand at 1081.106 kb on + strand at 1081.109 kb on - strand at 1081.109 kb on - strand at 1081.109 kb on - strand at 1081.138 kb on - strand at 1081.139 kb on + strand at 1081.139 kb on + strand at 1081.141 kb on - strand at 1081.142 kb on + strand at 1081.254 kb on - strand at 1081.254 kb on - strand at 1081.266 kb on - strand at 1081.266 kb on - strand at 1081.313 kb on + strand at 1081.333 kb on - strand at 1081.631 kb on + strand at 1081.663 kb on - strand at 1081.865 kb on + strand at 1081.895 kb on + strand at 1081.921 kb on + strand at 1081.958 kb on - strand at 1081.990 kb on - strand at 1082.085 kb on + strand at 1082.155 kb on - strand at 1082.160 kb on + strand at 1082.281 kb on - strand at 1082.331 kb on - strand at 1082.349 kb on - strand at 1082.427 kb on + strand at 1082.495 kb on - strand at 1082.534 kb on - strand at 1082.545 kb on + strand at 1082.590 kb on + strand at 1082.595 kb on - strand at 1082.785 kb on + strand, within MMP_RS05635 at 1082.859 kb on - strand, within MMP_RS05635 at 1082.948 kb on - strand, within MMP_RS05635 at 1082.948 kb on - strand, within MMP_RS05635 at 1082.948 kb on - strand, within MMP_RS05635 at 1082.956 kb on + strand, within MMP_RS05635 at 1082.972 kb on - strand, within MMP_RS05635 at 1082.972 kb on - strand, within MMP_RS05635 at 1083.024 kb on - strand, within MMP_RS05635 at 1083.088 kb on - strand, within MMP_RS05635 at 1083.108 kb on + strand, within MMP_RS05635 at 1083.217 kb on - strand, within MMP_RS05635 at 1083.309 kb on - strand, within MMP_RS05635 at 1083.314 kb on + strand, within MMP_RS05635 at 1083.352 kb on - strand at 1083.357 kb on - strand at 1083.358 kb on + strand at 1083.358 kb on + strand at 1083.397 kb on - strand at 1083.431 kb on - strand at 1083.431 kb on - strand at 1083.435 kb on + strand at 1083.435 kb on + strand at 1083.438 kb on - strand at 1083.438 kb on - strand at 1083.439 kb on + strand at 1083.439 kb on + strand at 1083.458 kb on - strand at 1083.458 kb on - strand at 1083.458 kb on - strand at 1083.459 kb on + strand at 1083.459 kb on + strand at 1083.469 kb on - strand at 1083.469 kb on - strand at 1083.472 kb on + strand at 1083.472 kb on + strand at 1083.472 kb on + strand at 1083.489 kb on + strand at 1083.489 kb on + strand at 1083.490 kb on - strand at 1083.490 kb on - strand at 1083.490 kb on - strand at 1083.491 kb on + strand at 1083.491 kb on + strand at 1083.491 kb on + strand at 1083.491 kb on + strand at 1083.497 kb on - strand at 1083.497 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 1,080,027 - MMP_RS05625 0.34 +0.1 1,080,087 - MMP_RS05625 0.39 +0.4 1,080,101 + MMP_RS05625 0.40 -0.5 1,080,141 - MMP_RS05625 0.43 -0.4 1,080,280 + MMP_RS05625 0.55 +0.4 1,080,303 - MMP_RS05625 0.57 -2.1 1,080,303 - MMP_RS05625 0.57 +0.6 1,080,306 - MMP_RS05625 0.57 -0.3 1,080,309 - MMP_RS05625 0.57 -0.1 1,080,309 - MMP_RS05625 0.57 -0.3 1,080,309 - MMP_RS05625 0.57 +1.0 1,080,310 + MMP_RS05625 0.57 -0.1 1,080,310 + MMP_RS05625 0.57 +0.8 1,080,325 + MMP_RS05625 0.59 +0.6 1,080,345 - MMP_RS05625 0.60 +0.2 1,080,346 + MMP_RS05625 0.60 -0.8 1,080,350 + MMP_RS05625 0.61 +0.1 1,080,351 - MMP_RS05625 0.61 +0.2 1,080,351 - MMP_RS05625 0.61 -0.5 1,080,352 + MMP_RS05625 0.61 +0.8 1,080,352 + MMP_RS05625 0.61 +0.8 1,080,356 + MMP_RS05625 0.61 +0.5 1,080,372 - MMP_RS05625 0.62 +0.5 1,080,399 - MMP_RS05625 0.65 -0.6 1,080,399 - MMP_RS05625 0.65 +0.2 1,080,399 - MMP_RS05625 0.65 -0.5 1,080,400 + MMP_RS05625 0.65 -0.1 1,080,401 - MMP_RS05625 0.65 +0.8 1,080,401 - MMP_RS05625 0.65 -0.1 1,080,402 + MMP_RS05625 0.65 +0.3 1,080,405 - MMP_RS05625 0.65 +0.2 1,080,409 + MMP_RS05625 0.66 -0.9 1,080,438 - MMP_RS05625 0.68 -0.4 1,080,475 - MMP_RS05625 0.71 +0.6 1,080,552 + MMP_RS05625 0.77 +0.5 1,080,588 - MMP_RS05625 0.80 +0.9 1,080,589 + MMP_RS05625 0.80 +0.2 1,080,589 + MMP_RS05625 0.80 -1.6 1,080,616 - MMP_RS05625 0.83 -1.1 1,080,616 - MMP_RS05625 0.83 +0.8 1,080,617 + MMP_RS05625 0.83 -1.2 1,080,621 - MMP_RS05625 0.83 -0.1 1,080,622 + MMP_RS05625 0.83 -1.3 1,080,622 + MMP_RS05625 0.83 -0.4 1,080,663 - MMP_RS05625 0.87 +0.6 1,080,667 + MMP_RS05625 0.87 -1.4 1,080,668 - MMP_RS05625 0.87 -0.1 1,080,670 - MMP_RS05625 0.87 -0.1 1,080,671 + MMP_RS05625 0.87 -0.3 1,080,691 - MMP_RS05625 0.89 -0.7 1,080,692 + MMP_RS05625 0.89 +0.1 1,080,692 + MMP_RS05625 0.89 +0.4 1,080,692 + MMP_RS05625 0.89 +0.1 1,080,692 + MMP_RS05625 0.89 -0.2 1,080,692 + MMP_RS05625 0.89 +1.4 1,080,693 - MMP_RS05625 0.89 +0.5 1,080,693 - MMP_RS05625 0.89 +0.4 1,080,694 + MMP_RS05625 0.89 +0.4 1,080,694 + MMP_RS05625 0.89 -0.3 1,080,694 + MMP_RS05625 0.89 -0.5 1,080,694 + MMP_RS05625 0.89 -0.9 1,080,705 - +1.5 1,080,751 - +0.5 1,080,756 + -0.2 1,080,770 + +1.4 1,080,804 - +0.6 1,080,804 - +1.5 1,080,804 - +0.3 1,080,804 - +0.9 1,080,805 + +0.3 1,080,805 + +0.7 1,080,805 + -0.9 1,080,805 + +0.4 1,080,806 - +0.2 1,080,806 - -0.0 1,080,806 - -0.6 1,080,806 - +0.5 1,080,807 + -1.0 1,080,807 + -0.4 1,080,807 + +0.0 1,080,807 + -0.2 1,080,807 + +0.5 1,080,808 - +0.8 1,080,808 - -0.2 1,080,809 + -0.5 1,080,809 + +0.3 1,080,809 + +0.0 1,080,858 - +0.5 1,080,858 - -0.3 1,080,859 + +0.2 1,080,860 - +0.7 1,080,864 + -0.0 1,080,876 - +0.4 1,080,877 + +0.8 1,080,901 - +0.1 1,080,908 + +0.6 1,080,913 + +0.2 1,080,959 + +0.0 1,080,959 + +0.0 1,080,979 - +0.4 1,080,985 + +0.2 1,081,001 - +0.8 1,081,031 + -2.2 1,081,106 + -2.1 1,081,106 + -3.8 1,081,109 - -3.0 1,081,109 - -2.1 1,081,109 - -1.4 1,081,138 - -3.1 1,081,139 + -2.9 1,081,139 + -0.7 1,081,141 - -2.0 1,081,142 + -2.1 1,081,254 - -1.1 1,081,254 - -2.3 1,081,266 - -1.9 1,081,266 - -3.1 1,081,313 + -0.6 1,081,333 - -1.4 1,081,631 + -1.7 1,081,663 - -3.3 1,081,865 + -1.1 1,081,895 + -1.7 1,081,921 + -1.7 1,081,958 - -2.1 1,081,990 - -2.6 1,082,085 + -2.5 1,082,155 - -1.7 1,082,160 + -2.7 1,082,281 - -0.1 1,082,331 - -2.4 1,082,349 - -1.8 1,082,427 + -1.5 1,082,495 - -2.8 1,082,534 - -2.7 1,082,545 + -0.6 1,082,590 + -0.1 1,082,595 - +0.4 1,082,785 + MMP_RS05635 0.29 -1.3 1,082,859 - MMP_RS05635 0.37 -0.7 1,082,948 - MMP_RS05635 0.47 +0.9 1,082,948 - MMP_RS05635 0.47 -1.8 1,082,948 - MMP_RS05635 0.47 -0.4 1,082,956 + MMP_RS05635 0.48 -0.5 1,082,972 - MMP_RS05635 0.50 -0.3 1,082,972 - MMP_RS05635 0.50 -0.6 1,083,024 - MMP_RS05635 0.56 -0.6 1,083,088 - MMP_RS05635 0.63 -0.2 1,083,108 + MMP_RS05635 0.65 +0.3 1,083,217 - MMP_RS05635 0.78 -0.2 1,083,309 - MMP_RS05635 0.88 -1.0 1,083,314 + MMP_RS05635 0.89 -1.6 1,083,352 - -0.8 1,083,357 - -0.1 1,083,358 + -1.4 1,083,358 + -0.2 1,083,397 - -0.3 1,083,431 - -1.0 1,083,431 - -0.6 1,083,435 + -0.3 1,083,435 + -0.8 1,083,438 - -0.8 1,083,438 - -0.4 1,083,439 + -0.0 1,083,439 + +0.1 1,083,458 - +0.5 1,083,458 - +0.2 1,083,458 - -0.3 1,083,459 + -0.5 1,083,459 + -0.8 1,083,469 - +0.2 1,083,469 - +0.1 1,083,472 + -1.2 1,083,472 + -2.2 1,083,472 + -0.5 1,083,489 + -1.7 1,083,489 + -0.4 1,083,490 - +0.1 1,083,490 - +0.5 1,083,490 - -0.0 1,083,491 + -0.8 1,083,491 + -0.8 1,083,491 + -0.1 1,083,491 + +0.5 1,083,497 - -0.3 1,083,497 - -0.2
Or see this region's nucleotide sequence