Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05565 and MMP_RS05570 are separated by 8 nucleotides MMP_RS05570 and MMP_RS05575 are separated by 94 nucleotides
MMP_RS05565: MMP_RS05565 - NTP transferase domain-containing protein, at 1,065,921 to 1,067,156
_RS05565
MMP_RS05570: MMP_RS05570 - phosphoglucosamine mutase, at 1,067,165 to 1,068,508
_RS05570
MMP_RS05575: MMP_RS05575 - hypothetical protein, at 1,068,603 to 1,069,547
_RS05575
Position (kb)
1067
1068
1069 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1066.258 kb on + strand at 1066.685 kb on - strand at 1066.688 kb on - strand at 1066.789 kb on + strand at 1066.884 kb on - strand at 1066.945 kb on - strand at 1066.972 kb on - strand at 1066.973 kb on + strand at 1067.012 kb on + strand at 1067.048 kb on - strand at 1067.056 kb on + strand at 1067.121 kb on + strand at 1067.504 kb on + strand at 1068.127 kb on - strand at 1068.127 kb on - strand at 1068.155 kb on - strand at 1068.323 kb on + strand at 1068.326 kb on - strand at 1068.327 kb on + strand at 1068.327 kb on + strand at 1068.375 kb on + strand at 1068.541 kb on + strand at 1068.548 kb on - strand at 1068.549 kb on + strand at 1068.549 kb on + strand at 1068.549 kb on + strand at 1068.568 kb on - strand at 1068.569 kb on + strand at 1068.572 kb on - strand at 1068.573 kb on + strand at 1068.574 kb on - strand at 1068.574 kb on - strand at 1068.574 kb on - strand at 1068.574 kb on - strand at 1068.641 kb on - strand at 1068.676 kb on - strand at 1068.683 kb on - strand at 1068.687 kb on - strand at 1068.687 kb on - strand at 1068.791 kb on - strand, within MMP_RS05575 at 1068.827 kb on - strand, within MMP_RS05575 at 1068.851 kb on + strand, within MMP_RS05575 at 1069.044 kb on - strand, within MMP_RS05575 at 1069.045 kb on + strand, within MMP_RS05575 at 1069.071 kb on + strand, within MMP_RS05575 at 1069.071 kb on + strand, within MMP_RS05575 at 1069.076 kb on + strand, within MMP_RS05575 at 1069.084 kb on + strand, within MMP_RS05575 at 1069.088 kb on - strand, within MMP_RS05575 at 1069.091 kb on + strand, within MMP_RS05575 at 1069.091 kb on + strand, within MMP_RS05575 at 1069.095 kb on + strand, within MMP_RS05575 at 1069.105 kb on - strand, within MMP_RS05575 at 1069.120 kb on - strand, within MMP_RS05575 at 1069.261 kb on + strand, within MMP_RS05575 at 1069.323 kb on - strand, within MMP_RS05575 at 1069.324 kb on + strand, within MMP_RS05575 at 1069.351 kb on + strand, within MMP_RS05575 at 1069.352 kb on - strand, within MMP_RS05575 at 1069.352 kb on - strand, within MMP_RS05575 at 1069.355 kb on - strand, within MMP_RS05575 at 1069.356 kb on + strand, within MMP_RS05575 at 1069.415 kb on + strand, within MMP_RS05575 at 1069.444 kb on - strand, within MMP_RS05575 at 1069.450 kb on - strand, within MMP_RS05575 at 1069.451 kb on + strand, within MMP_RS05575 at 1069.468 kb on - strand at 1069.469 kb on + strand at 1069.469 kb on + strand at 1069.473 kb on - strand at 1069.474 kb on + strand at 1069.474 kb on + strand at 1069.478 kb on - strand at 1069.479 kb on + strand at 1069.496 kb on - strand at 1069.496 kb on - strand at 1069.496 kb on - strand at 1069.500 kb on - strand at 1069.501 kb on + strand at 1069.501 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 1,066,258 + -2.4 1,066,685 - -2.6 1,066,688 - -2.1 1,066,789 + -3.0 1,066,884 - +0.9 1,066,945 - -2.9 1,066,972 - -2.8 1,066,973 + -4.1 1,067,012 + -1.3 1,067,048 - -1.9 1,067,056 + -1.7 1,067,121 + -0.6 1,067,504 + -0.9 1,068,127 - -1.0 1,068,127 - +0.4 1,068,155 - -0.4 1,068,323 + -1.6 1,068,326 - -1.3 1,068,327 + +0.1 1,068,327 + -3.3 1,068,375 + -0.4 1,068,541 + -0.1 1,068,548 - +1.5 1,068,549 + -4.4 1,068,549 + -0.8 1,068,549 + -0.9 1,068,568 - +0.4 1,068,569 + +0.9 1,068,572 - +2.9 1,068,573 + -0.6 1,068,574 - -0.2 1,068,574 - -1.3 1,068,574 - -0.3 1,068,574 - -0.1 1,068,641 - +0.4 1,068,676 - -0.2 1,068,683 - +0.4 1,068,687 - -0.0 1,068,687 - -0.8 1,068,791 - MMP_RS05575 0.20 +0.6 1,068,827 - MMP_RS05575 0.24 -1.0 1,068,851 + MMP_RS05575 0.26 -2.1 1,069,044 - MMP_RS05575 0.47 -0.2 1,069,045 + MMP_RS05575 0.47 +0.7 1,069,071 + MMP_RS05575 0.50 +0.7 1,069,071 + MMP_RS05575 0.50 -0.2 1,069,076 + MMP_RS05575 0.50 +2.2 1,069,084 + MMP_RS05575 0.51 -0.6 1,069,088 - MMP_RS05575 0.51 +0.2 1,069,091 + MMP_RS05575 0.52 -0.1 1,069,091 + MMP_RS05575 0.52 +0.4 1,069,095 + MMP_RS05575 0.52 -1.2 1,069,105 - MMP_RS05575 0.53 -1.0 1,069,120 - MMP_RS05575 0.55 +0.6 1,069,261 + MMP_RS05575 0.70 -1.0 1,069,323 - MMP_RS05575 0.76 +1.3 1,069,324 + MMP_RS05575 0.76 -0.2 1,069,351 + MMP_RS05575 0.79 -0.1 1,069,352 - MMP_RS05575 0.79 +0.4 1,069,352 - MMP_RS05575 0.79 +0.5 1,069,355 - MMP_RS05575 0.80 +0.4 1,069,356 + MMP_RS05575 0.80 +0.9 1,069,415 + MMP_RS05575 0.86 -0.8 1,069,444 - MMP_RS05575 0.89 -0.0 1,069,450 - MMP_RS05575 0.90 +1.4 1,069,451 + MMP_RS05575 0.90 +0.4 1,069,468 - +0.4 1,069,469 + +0.4 1,069,469 + -0.1 1,069,473 - -0.2 1,069,474 + +0.4 1,069,474 + +1.2 1,069,478 - +0.3 1,069,479 + -1.5 1,069,496 - +0.6 1,069,496 - -1.4 1,069,496 - +0.3 1,069,500 - +0.5 1,069,501 + -0.1 1,069,501 + +0.0
Or see this region's nucleotide sequence