Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05550 and MMP_RS05555 are separated by 9 nucleotides MMP_RS05555 and MMP_RS05560 are separated by 70 nucleotides MMP_RS05560 and MMP_RS05565 are separated by 1 nucleotides MMP_RS05565 and MMP_RS05570 are separated by 8 nucleotides
MMP_RS05550: MMP_RS05550 - cation:proton antiporter, at 1,064,872 to 1,065,126
_RS05550
MMP_RS05555: MMP_RS05555 - DUF4040 domain-containing protein, at 1,065,136 to 1,065,378
_RS05555
MMP_RS05560: MMP_RS05560 - deoxyuridine 5'-triphosphate nucleotidohydrolase, at 1,065,449 to 1,065,919
_RS05560
MMP_RS05565: MMP_RS05565 - NTP transferase domain-containing protein, at 1,065,921 to 1,067,156
_RS05565
MMP_RS05570: MMP_RS05570 - phosphoglucosamine mutase, at 1,067,165 to 1,068,508
_RS05570
Position (kb)
1065
1066
1067
1068 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1065.006 kb on - strand, within MMP_RS05550 at 1065.213 kb on + strand, within MMP_RS05555 at 1065.276 kb on + strand, within MMP_RS05555 at 1065.376 kb on - strand at 1065.381 kb on - strand at 1065.387 kb on - strand at 1065.457 kb on - strand at 1065.457 kb on - strand at 1065.463 kb on + strand at 1065.474 kb on - strand at 1065.474 kb on - strand at 1065.474 kb on - strand at 1065.528 kb on - strand, within MMP_RS05560 at 1065.528 kb on - strand, within MMP_RS05560 at 1065.533 kb on + strand, within MMP_RS05560 at 1065.533 kb on + strand, within MMP_RS05560 at 1065.535 kb on + strand, within MMP_RS05560 at 1065.555 kb on + strand, within MMP_RS05560 at 1065.607 kb on - strand, within MMP_RS05560 at 1065.608 kb on + strand, within MMP_RS05560 at 1065.608 kb on + strand, within MMP_RS05560 at 1065.610 kb on + strand, within MMP_RS05560 at 1065.706 kb on + strand, within MMP_RS05560 at 1065.706 kb on + strand, within MMP_RS05560 at 1065.706 kb on + strand, within MMP_RS05560 at 1065.706 kb on + strand, within MMP_RS05560 at 1065.902 kb on + strand at 1065.922 kb on - strand at 1065.922 kb on - strand at 1066.140 kb on - strand at 1066.258 kb on + strand at 1066.685 kb on - strand at 1066.688 kb on - strand at 1066.789 kb on + strand at 1066.884 kb on - strand at 1066.945 kb on - strand at 1066.972 kb on - strand at 1066.973 kb on + strand at 1067.012 kb on + strand at 1067.048 kb on - strand at 1067.056 kb on + strand at 1067.121 kb on + strand at 1067.504 kb on + strand at 1068.127 kb on - strand at 1068.127 kb on - strand at 1068.155 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 1,065,006 - MMP_RS05550 0.53 -5.2 1,065,213 + MMP_RS05555 0.32 -2.0 1,065,276 + MMP_RS05555 0.58 -1.1 1,065,376 - +0.7 1,065,381 - +0.4 1,065,387 - +0.7 1,065,457 - -1.6 1,065,457 - +0.0 1,065,463 + +0.2 1,065,474 - -2.0 1,065,474 - +0.6 1,065,474 - +0.1 1,065,528 - MMP_RS05560 0.17 +0.1 1,065,528 - MMP_RS05560 0.17 +0.1 1,065,533 + MMP_RS05560 0.18 -0.3 1,065,533 + MMP_RS05560 0.18 -0.3 1,065,535 + MMP_RS05560 0.18 -0.4 1,065,555 + MMP_RS05560 0.23 -1.5 1,065,607 - MMP_RS05560 0.34 +1.1 1,065,608 + MMP_RS05560 0.34 -1.5 1,065,608 + MMP_RS05560 0.34 -0.0 1,065,610 + MMP_RS05560 0.34 +0.7 1,065,706 + MMP_RS05560 0.55 -1.3 1,065,706 + MMP_RS05560 0.55 +0.5 1,065,706 + MMP_RS05560 0.55 -1.2 1,065,706 + MMP_RS05560 0.55 -1.0 1,065,902 + -0.1 1,065,922 - +0.6 1,065,922 - +0.4 1,066,140 - -4.2 1,066,258 + -2.4 1,066,685 - -2.6 1,066,688 - -2.1 1,066,789 + -3.0 1,066,884 - +0.9 1,066,945 - -2.9 1,066,972 - -2.8 1,066,973 + -4.1 1,067,012 + -1.3 1,067,048 - -1.9 1,067,056 + -1.7 1,067,121 + -0.6 1,067,504 + -0.9 1,068,127 - -1.0 1,068,127 - +0.4 1,068,155 - -0.4
Or see this region's nucleotide sequence