Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05380 and MMP_RS05385 are separated by 60 nucleotides MMP_RS05385 and MMP_RS05390 are separated by 10 nucleotides MMP_RS05390 and MMP_RS05395 are separated by 21 nucleotides MMP_RS05395 and MMP_RS05400 are separated by 15 nucleotides
MMP_RS05380: MMP_RS05380 - ATP synthase subunit K, at 1,033,866 to 1,034,534
_RS05380
MMP_RS05385: MMP_RS05385 - ATP synthase subunit E, at 1,034,595 to 1,035,206
_RS05385
MMP_RS05390: MMP_RS05390 - V-type ATP synthase subunit C, at 1,035,217 to 1,036,392
_RS05390
MMP_RS05395: MMP_RS05395 - V-type ATP synthase subunit F, at 1,036,414 to 1,036,713
_RS05395
MMP_RS05400: MMP_RS05400 - ATP synthase subunit A, at 1,036,729 to 1,038,489
_RS05400
Position (kb)
1035
1036
1037 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1034.959 kb on - strand at 1035.222 kb on + strand at 1035.419 kb on - strand at 1035.674 kb on + strand at 1036.040 kb on + strand at 1036.330 kb on + strand at 1036.597 kb on + strand at 1036.680 kb on + strand at 1037.140 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 1,034,959 - -2.2 1,035,222 + -4.5 1,035,419 - -3.9 1,035,674 + +0.8 1,036,040 + -4.7 1,036,330 + -4.3 1,036,597 + -1.9 1,036,680 + +1.8 1,037,140 + -4.2
Or see this region's nucleotide sequence