Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05325 and MMP_RS05330 are separated by 115 nucleotides MMP_RS05330 and MMP_RS05335 are separated by 61 nucleotides MMP_RS05335 and MMP_RS05340 are separated by 119 nucleotides
MMP_RS05325: MMP_RS05325 - hypothetical protein, at 1,021,630 to 1,021,989
_RS05325
MMP_RS05330: MMP_RS05330 - EMC3/TMCO1 family protein, at 1,022,105 to 1,022,701
_RS05330
MMP_RS05335: MMP_RS05335 - adenylate kinase, at 1,022,763 to 1,023,341
_RS05335
MMP_RS05340: MMP_RS05340 - OB-fold nucleic acid binding domain-containing protein, at 1,023,461 to 1,025,389
_RS05340
Position (kb)
1022
1023
1024 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1021.789 kb on + strand, within MMP_RS05325 at 1021.800 kb on + strand, within MMP_RS05325 at 1021.800 kb on + strand, within MMP_RS05325 at 1022.027 kb on - strand at 1022.027 kb on - strand at 1022.028 kb on + strand at 1022.107 kb on + strand at 1022.222 kb on - strand at 1022.730 kb on - strand at 1022.759 kb on - strand at 1022.759 kb on - strand at 1023.404 kb on - strand at 1023.418 kb on + strand at 1023.423 kb on - strand at 1023.424 kb on + strand at 1023.427 kb on - strand at 1023.427 kb on - strand at 1023.428 kb on + strand at 1023.463 kb on + strand at 1023.463 kb on + strand at 1023.495 kb on + strand at 1023.530 kb on - strand at 1023.561 kb on - strand at 1023.663 kb on - strand at 1023.670 kb on - strand at 1023.670 kb on - strand at 1023.804 kb on + strand at 1023.815 kb on - strand at 1023.905 kb on - strand at 1023.927 kb on + strand at 1023.998 kb on - strand at 1024.090 kb on - strand at 1024.204 kb on - strand at 1024.279 kb on - strand at 1024.309 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 1,021,789 + MMP_RS05325 0.44 +0.4 1,021,800 + MMP_RS05325 0.47 -0.5 1,021,800 + MMP_RS05325 0.47 -0.5 1,022,027 - -1.6 1,022,027 - +0.7 1,022,028 + -0.2 1,022,107 + -1.4 1,022,222 - -1.1 1,022,730 - -1.6 1,022,759 - -0.3 1,022,759 - -1.7 1,023,404 - -3.9 1,023,418 + +0.9 1,023,423 - -4.2 1,023,424 + -1.7 1,023,427 - -0.3 1,023,427 - -0.2 1,023,428 + +0.6 1,023,463 + +0.6 1,023,463 + -0.2 1,023,495 + -0.7 1,023,530 - -3.4 1,023,561 - -3.5 1,023,663 - -0.7 1,023,670 - -1.7 1,023,670 - -1.1 1,023,804 + -0.8 1,023,815 - -1.1 1,023,905 - -2.6 1,023,927 + -2.9 1,023,998 - -2.7 1,024,090 - -1.8 1,024,204 - -4.4 1,024,279 - -1.1 1,024,309 - -2.9
Or see this region's nucleotide sequence