Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05235 and MMP_RS05240 are separated by 214 nucleotides MMP_RS05240 and MMP_RS05245 are separated by 64 nucleotides
MMP_RS05235: MMP_RS05235 - exodeoxyribonuclease III, at 997,149 to 997,898
_RS05235
MMP_RS05240: MMP_RS05240 - carbamoyl-phosphate synthase large subunit, at 998,113 to 1,001,358
_RS05240
MMP_RS05245: MMP_RS05245 - archaeosine biosynthesis radical SAM protein RaSEA, at 1,001,423 to 1,002,490
_RS05245
Position (kb) 998
999
1000
1001
1002 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 997.151 kb on + strand at 997.158 kb on - strand at 997.159 kb on + strand at 997.160 kb on - strand at 997.160 kb on - strand at 997.192 kb on + strand at 997.204 kb on + strand at 997.204 kb on + strand at 997.205 kb on - strand at 997.205 kb on - strand at 997.205 kb on - strand at 997.205 kb on - strand at 997.205 kb on - strand at 997.205 kb on - strand at 997.205 kb on - strand at 997.205 kb on - strand at 997.205 kb on - strand at 997.205 kb on - strand at 997.205 kb on - strand at 997.205 kb on - strand at 997.206 kb on + strand at 997.206 kb on + strand at 997.206 kb on + strand at 997.206 kb on + strand at 997.206 kb on + strand at 997.206 kb on + strand at 997.206 kb on + strand at 997.206 kb on + strand at 997.206 kb on + strand at 997.206 kb on + strand at 997.206 kb on + strand at 997.206 kb on + strand at 997.206 kb on + strand at 997.207 kb on - strand at 997.207 kb on - strand at 997.207 kb on - strand at 997.208 kb on + strand at 997.208 kb on + strand at 997.208 kb on + strand at 997.244 kb on - strand, within MMP_RS05235 at 997.257 kb on - strand, within MMP_RS05235 at 997.258 kb on + strand, within MMP_RS05235 at 997.312 kb on - strand, within MMP_RS05235 at 997.317 kb on + strand, within MMP_RS05235 at 997.600 kb on - strand, within MMP_RS05235 at 997.603 kb on - strand, within MMP_RS05235 at 997.622 kb on - strand, within MMP_RS05235 at 997.800 kb on - strand, within MMP_RS05235 at 997.800 kb on - strand, within MMP_RS05235 at 997.801 kb on + strand, within MMP_RS05235 at 997.875 kb on - strand at 997.876 kb on + strand at 997.876 kb on + strand at 997.926 kb on + strand at 997.931 kb on + strand at 997.933 kb on - strand at 997.936 kb on + strand at 997.945 kb on + strand at 997.990 kb on + strand at 998.008 kb on + strand at 998.008 kb on + strand at 998.011 kb on - strand at 998.027 kb on - strand at 998.028 kb on + strand at 998.028 kb on + strand at 998.047 kb on - strand at 998.081 kb on - strand at 998.084 kb on - strand at 998.085 kb on + strand at 998.834 kb on - strand at 998.834 kb on - strand at 998.877 kb on + strand at 999.726 kb on - strand at 999.737 kb on + strand at 999.957 kb on - strand at 1000.648 kb on - strand at 1001.399 kb on + strand at 1001.562 kb on - strand, within MMP_RS05245 at 1001.584 kb on - strand, within MMP_RS05245 at 1001.585 kb on + strand, within MMP_RS05245 at 1001.585 kb on + strand, within MMP_RS05245 at 1001.595 kb on + strand, within MMP_RS05245 at 1001.716 kb on - strand, within MMP_RS05245 at 1001.722 kb on - strand, within MMP_RS05245 at 1001.722 kb on - strand, within MMP_RS05245 at 1001.722 kb on - strand, within MMP_RS05245 at 1001.723 kb on + strand, within MMP_RS05245 at 1002.039 kb on + strand, within MMP_RS05245 at 1002.062 kb on + strand, within MMP_RS05245 at 1002.112 kb on - strand, within MMP_RS05245 at 1002.113 kb on + strand, within MMP_RS05245 at 1002.113 kb on + strand, within MMP_RS05245 at 1002.115 kb on + strand, within MMP_RS05245 at 1002.115 kb on + strand, within MMP_RS05245 at 1002.172 kb on + strand, within MMP_RS05245 at 1002.316 kb on - strand, within MMP_RS05245 at 1002.357 kb on - strand, within MMP_RS05245 at 1002.357 kb on - strand, within MMP_RS05245
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 997,151 + +0.5 997,158 - +0.4 997,159 + -0.3 997,160 - -1.3 997,160 - +1.3 997,192 + -0.1 997,204 + -0.3 997,204 + -1.7 997,205 - -0.5 997,205 - +1.2 997,205 - +0.5 997,205 - +0.8 997,205 - +0.4 997,205 - +0.8 997,205 - -0.5 997,205 - +0.5 997,205 - -0.5 997,205 - +0.3 997,205 - -0.2 997,205 - +0.2 997,206 + -0.2 997,206 + +1.0 997,206 + +1.3 997,206 + -0.4 997,206 + +0.1 997,206 + +0.0 997,206 + +0.6 997,206 + -0.1 997,206 + -0.1 997,206 + +0.6 997,206 + +0.0 997,206 + -0.4 997,206 + +1.0 997,207 - +0.3 997,207 - -0.0 997,207 - +0.5 997,208 + +0.6 997,208 + -0.2 997,208 + -0.6 997,244 - MMP_RS05235 0.13 +0.2 997,257 - MMP_RS05235 0.14 +0.2 997,258 + MMP_RS05235 0.15 +0.9 997,312 - MMP_RS05235 0.22 +0.4 997,317 + MMP_RS05235 0.22 -0.1 997,600 - MMP_RS05235 0.60 +0.0 997,603 - MMP_RS05235 0.61 +0.3 997,622 - MMP_RS05235 0.63 +0.3 997,800 - MMP_RS05235 0.87 -0.1 997,800 - MMP_RS05235 0.87 +0.2 997,801 + MMP_RS05235 0.87 -0.2 997,875 - -0.5 997,876 + +0.1 997,876 + +0.6 997,926 + +0.2 997,931 + +0.1 997,933 - -0.6 997,936 + -1.0 997,945 + +0.4 997,990 + -0.4 998,008 + +1.1 998,008 + +0.3 998,011 - +0.8 998,027 - +0.7 998,028 + -0.5 998,028 + +0.8 998,047 - -1.6 998,081 - -3.5 998,084 - -2.7 998,085 + -3.7 998,834 - +0.6 998,834 - -3.6 998,877 + -2.6 999,726 - -0.9 999,737 + -2.5 999,957 - +1.8 1,000,648 - -4.0 1,001,399 + -1.0 1,001,562 - MMP_RS05245 0.13 -1.5 1,001,584 - MMP_RS05245 0.15 -0.7 1,001,585 + MMP_RS05245 0.15 +0.2 1,001,585 + MMP_RS05245 0.15 -1.8 1,001,595 + MMP_RS05245 0.16 -0.9 1,001,716 - MMP_RS05245 0.27 -0.6 1,001,722 - MMP_RS05245 0.28 +0.3 1,001,722 - MMP_RS05245 0.28 -0.4 1,001,722 - MMP_RS05245 0.28 -1.8 1,001,723 + MMP_RS05245 0.28 +0.1 1,002,039 + MMP_RS05245 0.58 -1.4 1,002,062 + MMP_RS05245 0.60 +0.6 1,002,112 - MMP_RS05245 0.65 +0.4 1,002,113 + MMP_RS05245 0.65 +0.6 1,002,113 + MMP_RS05245 0.65 -0.5 1,002,115 + MMP_RS05245 0.65 -0.1 1,002,115 + MMP_RS05245 0.65 -0.9 1,002,172 + MMP_RS05245 0.70 +0.8 1,002,316 - MMP_RS05245 0.84 +0.3 1,002,357 - MMP_RS05245 0.87 +0.1 1,002,357 - MMP_RS05245 0.87 -0.7
Or see this region's nucleotide sequence