Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05225 and MMP_RS05230 are separated by 110 nucleotides MMP_RS05230 and MMP_RS05235 are separated by 154 nucleotides
MMP_RS05225: MMP_RS05225 - RluA family pseudouridine synthase, at 994,323 to 995,216
_RS05225
MMP_RS05230: MMP_RS05230 - glutamate--tRNA ligase, at 995,327 to 996,994
_RS05230
MMP_RS05235: MMP_RS05235 - exodeoxyribonuclease III, at 997,149 to 997,898
_RS05235
Position (kb) 995
996
997 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 994.352 kb on - strand at 994.353 kb on + strand at 994.374 kb on + strand at 994.439 kb on - strand, within MMP_RS05225 at 994.439 kb on - strand, within MMP_RS05225 at 994.451 kb on - strand, within MMP_RS05225 at 994.451 kb on - strand, within MMP_RS05225 at 994.485 kb on + strand, within MMP_RS05225 at 994.504 kb on + strand, within MMP_RS05225 at 994.536 kb on + strand, within MMP_RS05225 at 994.539 kb on - strand, within MMP_RS05225 at 994.539 kb on - strand, within MMP_RS05225 at 994.539 kb on - strand, within MMP_RS05225 at 994.540 kb on + strand, within MMP_RS05225 at 994.588 kb on - strand, within MMP_RS05225 at 994.592 kb on - strand, within MMP_RS05225 at 994.699 kb on + strand, within MMP_RS05225 at 994.709 kb on - strand, within MMP_RS05225 at 994.709 kb on - strand, within MMP_RS05225 at 994.710 kb on + strand, within MMP_RS05225 at 994.712 kb on + strand, within MMP_RS05225 at 994.712 kb on + strand, within MMP_RS05225 at 994.712 kb on + strand, within MMP_RS05225 at 994.713 kb on - strand, within MMP_RS05225 at 994.765 kb on - strand, within MMP_RS05225 at 994.766 kb on + strand, within MMP_RS05225 at 994.932 kb on + strand, within MMP_RS05225 at 994.960 kb on - strand, within MMP_RS05225 at 994.971 kb on - strand, within MMP_RS05225 at 994.972 kb on + strand, within MMP_RS05225 at 995.218 kb on - strand at 995.219 kb on + strand at 995.219 kb on + strand at 995.259 kb on - strand at 995.274 kb on - strand at 995.320 kb on - strand at 995.322 kb on - strand at 995.339 kb on - strand at 995.353 kb on + strand at 995.353 kb on + strand at 995.356 kb on - strand at 995.670 kb on + strand at 996.438 kb on - strand at 996.909 kb on + strand at 996.972 kb on - strand at 996.972 kb on - strand at 996.973 kb on + strand at 996.981 kb on + strand at 996.994 kb on - strand at 996.999 kb on + strand at 997.010 kb on - strand at 997.043 kb on - strand at 997.102 kb on - strand at 997.109 kb on + strand at 997.151 kb on + strand at 997.158 kb on - strand at 997.159 kb on + strand at 997.160 kb on - strand at 997.160 kb on - strand at 997.192 kb on + strand at 997.204 kb on + strand at 997.204 kb on + strand at 997.205 kb on - strand at 997.205 kb on - strand at 997.205 kb on - strand at 997.205 kb on - strand at 997.205 kb on - strand at 997.205 kb on - strand at 997.205 kb on - strand at 997.205 kb on - strand at 997.205 kb on - strand at 997.205 kb on - strand at 997.205 kb on - strand at 997.205 kb on - strand at 997.206 kb on + strand at 997.206 kb on + strand at 997.206 kb on + strand at 997.206 kb on + strand at 997.206 kb on + strand at 997.206 kb on + strand at 997.206 kb on + strand at 997.206 kb on + strand at 997.206 kb on + strand at 997.206 kb on + strand at 997.206 kb on + strand at 997.206 kb on + strand at 997.206 kb on + strand at 997.207 kb on - strand at 997.207 kb on - strand at 997.207 kb on - strand at 997.208 kb on + strand at 997.208 kb on + strand at 997.208 kb on + strand at 997.244 kb on - strand, within MMP_RS05235 at 997.257 kb on - strand, within MMP_RS05235 at 997.258 kb on + strand, within MMP_RS05235 at 997.312 kb on - strand, within MMP_RS05235 at 997.317 kb on + strand, within MMP_RS05235 at 997.600 kb on - strand, within MMP_RS05235 at 997.603 kb on - strand, within MMP_RS05235 at 997.622 kb on - strand, within MMP_RS05235 at 997.800 kb on - strand, within MMP_RS05235 at 997.800 kb on - strand, within MMP_RS05235 at 997.801 kb on + strand, within MMP_RS05235 at 997.875 kb on - strand at 997.876 kb on + strand at 997.876 kb on + strand at 997.926 kb on + strand at 997.931 kb on + strand at 997.933 kb on - strand at 997.936 kb on + strand at 997.945 kb on + strand at 997.990 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 994,352 - +0.1 994,353 + +0.9 994,374 + +0.5 994,439 - MMP_RS05225 0.13 +0.4 994,439 - MMP_RS05225 0.13 -0.3 994,451 - MMP_RS05225 0.14 +1.2 994,451 - MMP_RS05225 0.14 +1.0 994,485 + MMP_RS05225 0.18 +0.5 994,504 + MMP_RS05225 0.20 +0.8 994,536 + MMP_RS05225 0.24 +1.3 994,539 - MMP_RS05225 0.24 +0.7 994,539 - MMP_RS05225 0.24 -0.3 994,539 - MMP_RS05225 0.24 +0.2 994,540 + MMP_RS05225 0.24 +0.6 994,588 - MMP_RS05225 0.30 +1.0 994,592 - MMP_RS05225 0.30 +0.6 994,699 + MMP_RS05225 0.42 +0.1 994,709 - MMP_RS05225 0.43 -0.7 994,709 - MMP_RS05225 0.43 -0.7 994,710 + MMP_RS05225 0.43 -0.5 994,712 + MMP_RS05225 0.44 -0.4 994,712 + MMP_RS05225 0.44 -0.7 994,712 + MMP_RS05225 0.44 -1.0 994,713 - MMP_RS05225 0.44 +0.2 994,765 - MMP_RS05225 0.49 +1.4 994,766 + MMP_RS05225 0.50 -0.2 994,932 + MMP_RS05225 0.68 -0.1 994,960 - MMP_RS05225 0.71 -0.2 994,971 - MMP_RS05225 0.72 -0.8 994,972 + MMP_RS05225 0.73 +0.1 995,218 - -0.9 995,219 + +0.4 995,219 + +0.3 995,259 - +0.8 995,274 - +0.2 995,320 - -0.1 995,322 - +0.8 995,339 - -0.8 995,353 + -4.2 995,353 + -2.9 995,356 - -3.3 995,670 + -1.6 996,438 - -2.2 996,909 + -2.6 996,972 - -2.9 996,972 - -0.2 996,973 + -3.7 996,981 + -4.5 996,994 - -3.0 996,999 + -0.8 997,010 - -3.3 997,043 - -0.4 997,102 - +0.5 997,109 + +0.2 997,151 + +0.5 997,158 - +0.4 997,159 + -0.3 997,160 - -1.3 997,160 - +1.3 997,192 + -0.1 997,204 + -0.3 997,204 + -1.7 997,205 - -0.5 997,205 - +1.2 997,205 - +0.5 997,205 - +0.8 997,205 - +0.4 997,205 - +0.8 997,205 - -0.5 997,205 - +0.5 997,205 - -0.5 997,205 - +0.3 997,205 - -0.2 997,205 - +0.2 997,206 + -0.2 997,206 + +1.0 997,206 + +1.3 997,206 + -0.4 997,206 + +0.1 997,206 + +0.0 997,206 + +0.6 997,206 + -0.1 997,206 + -0.1 997,206 + +0.6 997,206 + +0.0 997,206 + -0.4 997,206 + +1.0 997,207 - +0.3 997,207 - -0.0 997,207 - +0.5 997,208 + +0.6 997,208 + -0.2 997,208 + -0.6 997,244 - MMP_RS05235 0.13 +0.2 997,257 - MMP_RS05235 0.14 +0.2 997,258 + MMP_RS05235 0.15 +0.9 997,312 - MMP_RS05235 0.22 +0.4 997,317 + MMP_RS05235 0.22 -0.1 997,600 - MMP_RS05235 0.60 +0.0 997,603 - MMP_RS05235 0.61 +0.3 997,622 - MMP_RS05235 0.63 +0.3 997,800 - MMP_RS05235 0.87 -0.1 997,800 - MMP_RS05235 0.87 +0.2 997,801 + MMP_RS05235 0.87 -0.2 997,875 - -0.5 997,876 + +0.1 997,876 + +0.6 997,926 + +0.2 997,931 + +0.1 997,933 - -0.6 997,936 + -1.0 997,945 + +0.4 997,990 + -0.4
Or see this region's nucleotide sequence