Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05205 and MMP_RS05210 overlap by 8 nucleotides MMP_RS05210 and MMP_RS05215 overlap by 1 nucleotides MMP_RS05215 and MMP_RS05220 are separated by 331 nucleotides
MMP_RS05205: MMP_RS05205 - anthranilate synthase component I, at 989,744 to 991,063
_RS05205
MMP_RS05210: MMP_RS05210 - anthranilate phosphoribosyltransferase, at 991,056 to 992,021
_RS05210
MMP_RS05215: MMP_RS05215 - indole-3-glycerol phosphate synthase TrpC, at 992,021 to 992,710
_RS05215
MMP_RS05220: MMP_RS05220 - dihydroorotase, at 993,042 to 994,298
_RS05220
Position (kb)
992
993 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 991.085 kb on - strand at 991.160 kb on - strand at 991.393 kb on + strand at 991.523 kb on - strand at 991.634 kb on - strand at 992.540 kb on + strand at 992.541 kb on - strand at 992.573 kb on - strand at 992.740 kb on + strand at 992.740 kb on + strand at 992.813 kb on - strand at 992.813 kb on - strand at 992.813 kb on - strand at 992.813 kb on - strand at 992.814 kb on + strand at 992.814 kb on + strand at 992.871 kb on - strand at 992.951 kb on + strand at 992.965 kb on - strand at 992.986 kb on + strand at 993.025 kb on + strand at 993.046 kb on + strand at 993.048 kb on - strand at 993.050 kb on - strand at 993.051 kb on + strand at 993.051 kb on + strand at 993.052 kb on - strand at 993.052 kb on - strand at 993.052 kb on - strand at 993.052 kb on - strand at 993.053 kb on + strand at 993.053 kb on + strand at 993.053 kb on + strand at 993.054 kb on - strand at 993.055 kb on + strand at 993.185 kb on - strand at 993.212 kb on - strand at 993.260 kb on + strand at 993.260 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 991,085 - -3.3 991,160 - -2.2 991,393 + -3.2 991,523 - -2.6 991,634 - +0.4 992,540 + -3.0 992,541 - -1.2 992,573 - -0.3 992,740 + -2.6 992,740 + -5.1 992,813 - -1.5 992,813 - -1.4 992,813 - -0.6 992,813 - -1.0 992,814 + -4.2 992,814 + -1.7 992,871 - -2.9 992,951 + +0.8 992,965 - -1.2 992,986 + -2.5 993,025 + -2.5 993,046 + -1.5 993,048 - -1.7 993,050 - -1.2 993,051 + -0.6 993,051 + +1.1 993,052 - +0.9 993,052 - -0.1 993,052 - -0.3 993,052 - +0.6 993,053 + +0.2 993,053 + -0.5 993,053 + +0.8 993,054 - +0.1 993,055 + +0.2 993,185 - -2.7 993,212 - -3.0 993,260 + -1.4 993,260 + -3.0
Or see this region's nucleotide sequence