Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05195 and MMP_RS05200 are separated by 9 nucleotides MMP_RS05200 and MMP_RS05205 overlap by 20 nucleotides MMP_RS05205 and MMP_RS05210 overlap by 8 nucleotides MMP_RS05210 and MMP_RS05215 overlap by 1 nucleotides
MMP_RS05195: MMP_RS05195 - phosphoribosylanthranilate isomerase, at 988,522 to 989,157
_RS05195
MMP_RS05200: MMP_RS05200 - aminodeoxychorismate/anthranilate synthase component II, at 989,167 to 989,763
_RS05200
MMP_RS05205: MMP_RS05205 - anthranilate synthase component I, at 989,744 to 991,063
_RS05205
MMP_RS05210: MMP_RS05210 - anthranilate phosphoribosyltransferase, at 991,056 to 992,021
_RS05210
MMP_RS05215: MMP_RS05215 - indole-3-glycerol phosphate synthase TrpC, at 992,021 to 992,710
_RS05215
Position (kb) 989
990
991
992 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 988.930 kb on - strand at 988.934 kb on - strand at 989.192 kb on - strand at 989.193 kb on + strand at 989.451 kb on - strand at 989.548 kb on - strand at 990.062 kb on - strand at 990.129 kb on + strand at 990.296 kb on + strand at 990.514 kb on + strand at 990.539 kb on - strand at 990.709 kb on + strand at 990.709 kb on + strand at 990.788 kb on + strand at 990.973 kb on - strand at 991.085 kb on - strand at 991.160 kb on - strand at 991.393 kb on + strand at 991.523 kb on - strand at 991.634 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 988,930 - -2.4 988,934 - -1.4 989,192 - -0.7 989,193 + -3.4 989,451 - -1.7 989,548 - -3.4 990,062 - -1.4 990,129 + -2.0 990,296 + -2.6 990,514 + -1.4 990,539 - -3.7 990,709 + -4.9 990,709 + -1.2 990,788 + -3.2 990,973 - -2.1 991,085 - -3.3 991,160 - -2.2 991,393 + -3.2 991,523 - -2.6 991,634 - +0.4
Or see this region's nucleotide sequence