Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05190 and MMP_RS05195 overlap by 11 nucleotides MMP_RS05195 and MMP_RS05200 are separated by 9 nucleotides MMP_RS05200 and MMP_RS05205 overlap by 20 nucleotides
MMP_RS05190: MMP_RS05190 - tryptophan synthase subunit beta, at 987,348 to 988,532
_RS05190
MMP_RS05195: MMP_RS05195 - phosphoribosylanthranilate isomerase, at 988,522 to 989,157
_RS05195
MMP_RS05200: MMP_RS05200 - aminodeoxychorismate/anthranilate synthase component II, at 989,167 to 989,763
_RS05200
MMP_RS05205: MMP_RS05205 - anthranilate synthase component I, at 989,744 to 991,063
_RS05205
Position (kb) 988
989
990 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 987.644 kb on + strand at 987.682 kb on - strand at 987.683 kb on + strand at 987.975 kb on - strand at 988.549 kb on - strand at 988.637 kb on + strand at 988.930 kb on - strand at 988.934 kb on - strand at 989.192 kb on - strand at 989.193 kb on + strand at 989.451 kb on - strand at 989.548 kb on - strand at 990.062 kb on - strand at 990.129 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 987,644 + -1.7 987,682 - -1.0 987,683 + -3.0 987,975 - -2.4 988,549 - -0.4 988,637 + -4.5 988,930 - -2.4 988,934 - -1.4 989,192 - -0.7 989,193 + -3.4 989,451 - -1.7 989,548 - -3.4 990,062 - -1.4 990,129 + -2.0
Or see this region's nucleotide sequence