Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05115 and MMP_RS05120 are separated by 143 nucleotides MMP_RS05120 and MMP_RS05125 are separated by 328 nucleotides
MMP_RS05115: MMP_RS05115 - 50S ribosomal protein L11 methyltransferase, at 974,237 to 975,124
_RS05115
MMP_RS05120: MMP_RS05120 - DNA topoisomerase VI subunit B, at 975,268 to 977,262
_RS05120
MMP_RS05125: MMP_RS05125 - D-aminoacyl-tRNA deacylase, at 977,591 to 978,355
_RS05125
Position (kb)
975
976
977
978 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 974.365 kb on - strand, within MMP_RS05115 at 974.413 kb on - strand, within MMP_RS05115 at 974.413 kb on - strand, within MMP_RS05115 at 974.413 kb on - strand, within MMP_RS05115 at 974.413 kb on - strand, within MMP_RS05115 at 974.414 kb on + strand, within MMP_RS05115 at 974.415 kb on - strand, within MMP_RS05115 at 974.416 kb on + strand, within MMP_RS05115 at 974.416 kb on + strand, within MMP_RS05115 at 974.416 kb on + strand, within MMP_RS05115 at 974.416 kb on + strand, within MMP_RS05115 at 974.417 kb on - strand, within MMP_RS05115 at 974.417 kb on - strand, within MMP_RS05115 at 974.417 kb on - strand, within MMP_RS05115 at 974.417 kb on - strand, within MMP_RS05115 at 974.418 kb on + strand, within MMP_RS05115 at 974.418 kb on + strand, within MMP_RS05115 at 974.418 kb on + strand, within MMP_RS05115 at 974.420 kb on + strand, within MMP_RS05115 at 974.623 kb on - strand, within MMP_RS05115 at 974.649 kb on + strand, within MMP_RS05115 at 974.936 kb on + strand, within MMP_RS05115 at 974.970 kb on + strand, within MMP_RS05115 at 974.970 kb on + strand, within MMP_RS05115 at 975.037 kb on - strand at 975.237 kb on - strand at 975.273 kb on - strand at 975.522 kb on - strand at 975.742 kb on - strand at 976.121 kb on - strand at 976.307 kb on - strand at 976.318 kb on - strand at 976.353 kb on + strand at 976.415 kb on + strand at 976.422 kb on + strand at 976.579 kb on - strand at 976.703 kb on - strand at 977.280 kb on - strand at 977.283 kb on + strand at 977.287 kb on - strand at 977.371 kb on - strand at 977.371 kb on - strand at 977.372 kb on + strand at 977.372 kb on + strand at 977.372 kb on + strand at 977.384 kb on + strand at 977.385 kb on - strand at 977.385 kb on - strand at 977.385 kb on - strand at 977.386 kb on + strand at 977.386 kb on + strand at 977.386 kb on + strand at 977.387 kb on - strand at 977.387 kb on - strand at 977.387 kb on - strand at 977.388 kb on + strand at 977.388 kb on + strand at 977.388 kb on + strand at 977.389 kb on - strand at 977.425 kb on + strand at 977.427 kb on - strand at 977.436 kb on - strand at 977.450 kb on + strand at 977.503 kb on - strand at 977.527 kb on - strand at 977.528 kb on + strand at 977.529 kb on - strand at 977.530 kb on + strand at 977.534 kb on + strand at 977.604 kb on - strand at 977.673 kb on - strand, within MMP_RS05125 at 977.673 kb on - strand, within MMP_RS05125 at 977.708 kb on - strand, within MMP_RS05125 at 977.735 kb on - strand, within MMP_RS05125 at 977.736 kb on + strand, within MMP_RS05125 at 977.736 kb on + strand, within MMP_RS05125 at 977.736 kb on + strand, within MMP_RS05125 at 977.736 kb on + strand, within MMP_RS05125 at 977.737 kb on - strand, within MMP_RS05125 at 977.739 kb on - strand, within MMP_RS05125 at 977.740 kb on + strand, within MMP_RS05125 at 977.740 kb on + strand, within MMP_RS05125 at 977.787 kb on - strand, within MMP_RS05125 at 977.788 kb on + strand, within MMP_RS05125 at 977.791 kb on - strand, within MMP_RS05125 at 977.791 kb on - strand, within MMP_RS05125 at 977.791 kb on - strand, within MMP_RS05125 at 977.792 kb on + strand, within MMP_RS05125 at 977.952 kb on - strand, within MMP_RS05125 at 978.072 kb on - strand, within MMP_RS05125 at 978.137 kb on - strand, within MMP_RS05125 at 978.193 kb on + strand, within MMP_RS05125 at 978.214 kb on + strand, within MMP_RS05125 at 978.224 kb on + strand, within MMP_RS05125
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 974,365 - MMP_RS05115 0.14 +0.1 974,413 - MMP_RS05115 0.20 -0.2 974,413 - MMP_RS05115 0.20 +0.6 974,413 - MMP_RS05115 0.20 +0.2 974,413 - MMP_RS05115 0.20 +1.0 974,414 + MMP_RS05115 0.20 -0.3 974,415 - MMP_RS05115 0.20 -0.0 974,416 + MMP_RS05115 0.20 -0.2 974,416 + MMP_RS05115 0.20 +1.2 974,416 + MMP_RS05115 0.20 +0.8 974,416 + MMP_RS05115 0.20 +0.9 974,417 - MMP_RS05115 0.20 -0.4 974,417 - MMP_RS05115 0.20 -1.2 974,417 - MMP_RS05115 0.20 +0.0 974,417 - MMP_RS05115 0.20 -0.2 974,418 + MMP_RS05115 0.20 -0.6 974,418 + MMP_RS05115 0.20 -0.4 974,418 + MMP_RS05115 0.20 +1.1 974,420 + MMP_RS05115 0.21 -0.4 974,623 - MMP_RS05115 0.43 +0.1 974,649 + MMP_RS05115 0.46 -1.5 974,936 + MMP_RS05115 0.79 -0.4 974,970 + MMP_RS05115 0.83 +1.0 974,970 + MMP_RS05115 0.83 +0.3 975,037 - +0.8 975,237 - -0.8 975,273 - -0.3 975,522 - -2.3 975,742 - -1.3 976,121 - -4.4 976,307 - -4.5 976,318 - -2.7 976,353 + -3.0 976,415 + -2.4 976,422 + -3.4 976,579 - -2.5 976,703 - -1.8 977,280 - -1.8 977,283 + -1.2 977,287 - -3.8 977,371 - -0.5 977,371 - +0.9 977,372 + -0.6 977,372 + +1.2 977,372 + -0.4 977,384 + +0.6 977,385 - -1.7 977,385 - -0.2 977,385 - -2.2 977,386 + -0.6 977,386 + -0.0 977,386 + +2.7 977,387 - +0.5 977,387 - +0.6 977,387 - -0.8 977,388 + -0.8 977,388 + -0.9 977,388 + -0.2 977,389 - +0.6 977,425 + +0.8 977,427 - -0.2 977,436 - +1.0 977,450 + -1.4 977,503 - -0.8 977,527 - +0.2 977,528 + -1.8 977,529 - +0.1 977,530 + +0.3 977,534 + +1.4 977,604 - -0.1 977,673 - MMP_RS05125 0.11 +0.2 977,673 - MMP_RS05125 0.11 -0.3 977,708 - MMP_RS05125 0.15 +0.4 977,735 - MMP_RS05125 0.19 -0.3 977,736 + MMP_RS05125 0.19 +0.8 977,736 + MMP_RS05125 0.19 -0.0 977,736 + MMP_RS05125 0.19 +0.1 977,736 + MMP_RS05125 0.19 -0.2 977,737 - MMP_RS05125 0.19 -1.6 977,739 - MMP_RS05125 0.19 +0.4 977,740 + MMP_RS05125 0.19 -0.4 977,740 + MMP_RS05125 0.19 -1.2 977,787 - MMP_RS05125 0.26 -0.0 977,788 + MMP_RS05125 0.26 -1.2 977,791 - MMP_RS05125 0.26 +0.6 977,791 - MMP_RS05125 0.26 -0.6 977,791 - MMP_RS05125 0.26 +0.0 977,792 + MMP_RS05125 0.26 -0.2 977,952 - MMP_RS05125 0.47 +0.9 978,072 - MMP_RS05125 0.63 -0.2 978,137 - MMP_RS05125 0.71 -0.7 978,193 + MMP_RS05125 0.79 +0.5 978,214 + MMP_RS05125 0.81 +0.0 978,224 + MMP_RS05125 0.83 +0.1
Or see this region's nucleotide sequence