Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05025 and MMP_RS05030 are separated by 21 nucleotides MMP_RS05030 and MMP_RS05035 are separated by 49 nucleotides MMP_RS05035 and MMP_RS05040 are separated by 77 nucleotides
MMP_RS05025: MMP_RS05025 - ATP-dependent DNA ligase, at 956,353 to 958,074
_RS05025
MMP_RS05030: MMP_RS05030 - adenylosuccinate lyase, at 958,096 to 959,445
_RS05030
MMP_RS05035: MMP_RS05035 - hypothetical protein, at 959,495 to 959,908
_RS05035
MMP_RS05040: MMP_RS05040 - permease, at 959,986 to 960,843
_RS05040
Position (kb) 958
959
960 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 957.333 kb on - strand at 957.463 kb on + strand at 957.657 kb on - strand at 957.657 kb on - strand at 957.772 kb on + strand at 957.901 kb on + strand at 958.020 kb on - strand at 958.023 kb on - strand at 958.023 kb on - strand at 958.029 kb on + strand at 958.055 kb on - strand at 958.055 kb on - strand at 958.072 kb on - strand at 958.093 kb on - strand at 958.119 kb on - strand at 958.381 kb on - strand at 958.615 kb on + strand at 959.103 kb on + strand at 959.206 kb on + strand at 959.206 kb on + strand at 959.206 kb on + strand at 959.338 kb on - strand at 959.339 kb on + strand at 959.384 kb on + strand at 959.443 kb on - strand at 959.463 kb on + strand at 959.469 kb on - strand at 959.469 kb on - strand at 959.469 kb on - strand at 959.470 kb on + strand at 959.470 kb on + strand at 959.507 kb on + strand at 959.508 kb on - strand at 959.513 kb on - strand at 959.513 kb on - strand at 959.569 kb on + strand, within MMP_RS05035 at 959.574 kb on + strand, within MMP_RS05035 at 959.591 kb on + strand, within MMP_RS05035 at 959.663 kb on + strand, within MMP_RS05035 at 959.666 kb on - strand, within MMP_RS05035 at 959.734 kb on - strand, within MMP_RS05035 at 959.738 kb on - strand, within MMP_RS05035 at 959.739 kb on + strand, within MMP_RS05035 at 959.739 kb on + strand, within MMP_RS05035 at 959.911 kb on - strand at 959.925 kb on + strand at 959.935 kb on - strand at 959.939 kb on + strand at 959.952 kb on - strand at 959.952 kb on - strand at 959.952 kb on - strand at 959.952 kb on - strand at 959.953 kb on + strand at 959.953 kb on + strand at 959.953 kb on + strand at 959.953 kb on + strand at 959.953 kb on + strand at 959.954 kb on - strand at 959.956 kb on - strand at 959.956 kb on - strand at 959.957 kb on + strand at 959.957 kb on + strand at 959.997 kb on - strand at 959.997 kb on - strand at 959.998 kb on + strand at 959.998 kb on + strand at 959.998 kb on + strand at 960.030 kb on - strand at 960.030 kb on - strand at 960.035 kb on + strand at 960.048 kb on + strand at 960.107 kb on + strand, within MMP_RS05040 at 960.107 kb on + strand, within MMP_RS05040 at 960.139 kb on + strand, within MMP_RS05040 at 960.174 kb on - strand, within MMP_RS05040 at 960.175 kb on + strand, within MMP_RS05040 at 960.175 kb on + strand, within MMP_RS05040 at 960.175 kb on + strand, within MMP_RS05040 at 960.208 kb on - strand, within MMP_RS05040 at 960.208 kb on - strand, within MMP_RS05040 at 960.209 kb on + strand, within MMP_RS05040 at 960.210 kb on - strand, within MMP_RS05040 at 960.210 kb on - strand, within MMP_RS05040 at 960.239 kb on - strand, within MMP_RS05040 at 960.246 kb on - strand, within MMP_RS05040 at 960.246 kb on - strand, within MMP_RS05040 at 960.267 kb on + strand, within MMP_RS05040 at 960.294 kb on + strand, within MMP_RS05040
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 957,333 - -3.0 957,463 + -4.4 957,657 - -2.9 957,657 - -1.3 957,772 + -2.3 957,901 + -5.4 958,020 - -2.2 958,023 - -0.7 958,023 - -2.2 958,029 + -0.2 958,055 - -2.3 958,055 - -2.4 958,072 - -2.6 958,093 - -1.8 958,119 - -1.2 958,381 - +1.0 958,615 + -1.3 959,103 + -1.8 959,206 + -2.4 959,206 + -2.3 959,206 + -4.1 959,338 - -3.6 959,339 + -1.3 959,384 + -1.6 959,443 - +0.3 959,463 + -0.1 959,469 - +1.2 959,469 - +0.3 959,469 - +1.5 959,470 + +0.0 959,470 + +0.8 959,507 + -0.6 959,508 - +0.5 959,513 - +0.4 959,513 - +0.0 959,569 + MMP_RS05035 0.18 +0.2 959,574 + MMP_RS05035 0.19 -0.1 959,591 + MMP_RS05035 0.23 +0.5 959,663 + MMP_RS05035 0.41 +0.0 959,666 - MMP_RS05035 0.41 -1.1 959,734 - MMP_RS05035 0.58 +0.8 959,738 - MMP_RS05035 0.59 +0.4 959,739 + MMP_RS05035 0.59 +0.5 959,739 + MMP_RS05035 0.59 +0.2 959,911 - -0.6 959,925 + -0.3 959,935 - +0.5 959,939 + +0.7 959,952 - -0.2 959,952 - +0.6 959,952 - +0.2 959,952 - +0.1 959,953 + -0.1 959,953 + +0.4 959,953 + +0.8 959,953 + +0.7 959,953 + +0.3 959,954 - -0.8 959,956 - -0.2 959,956 - -0.9 959,957 + -0.3 959,957 + -1.7 959,997 - -0.3 959,997 - +0.5 959,998 + +0.1 959,998 + +0.6 959,998 + -0.0 960,030 - +0.7 960,030 - -1.0 960,035 + +0.0 960,048 + +0.1 960,107 + MMP_RS05040 0.14 +0.7 960,107 + MMP_RS05040 0.14 -0.1 960,139 + MMP_RS05040 0.18 -1.0 960,174 - MMP_RS05040 0.22 -0.7 960,175 + MMP_RS05040 0.22 +0.0 960,175 + MMP_RS05040 0.22 +0.5 960,175 + MMP_RS05040 0.22 +0.2 960,208 - MMP_RS05040 0.26 -0.1 960,208 - MMP_RS05040 0.26 -0.8 960,209 + MMP_RS05040 0.26 +0.6 960,210 - MMP_RS05040 0.26 +0.2 960,210 - MMP_RS05040 0.26 -0.1 960,239 - MMP_RS05040 0.29 +0.5 960,246 - MMP_RS05040 0.30 -0.7 960,246 - MMP_RS05040 0.30 +0.0 960,267 + MMP_RS05040 0.33 -0.0 960,294 + MMP_RS05040 0.36 -0.2
Or see this region's nucleotide sequence