Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS04855 and MMP_RS04860 are separated by 16 nucleotides MMP_RS04860 and MMP_RS04865 are separated by 53 nucleotides MMP_RS04865 and MMP_RS04870 are separated by 15 nucleotides MMP_RS04870 and MMP_RS04875 are separated by 22 nucleotides
MMP_RS04855: MMP_RS04855 - shikimate dehydrogenase, at 923,937 to 924,773
_RS04855
MMP_RS04860: MMP_RS04860 - coenzyme F420-0:L-glutamate ligase, at 924,790 to 925,536
_RS04860
MMP_RS04865: MMP_RS04865 - adenosylcobinamide-GDP ribazoletransferase, at 925,590 to 926,363
_RS04865
MMP_RS04870: MMP_RS04870 - TIGR02253 family HAD-type hydrolase, at 926,379 to 927,056
_RS04870
MMP_RS04875: MMP_RS04875 - energy-converting hydrogenase B subunit P, at 927,079 to 927,366
_RS04875
Position (kb) 925
926
927 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 924.683 kb on + strand at 924.725 kb on + strand at 924.756 kb on + strand at 924.819 kb on + strand at 924.876 kb on - strand at 924.908 kb on - strand at 924.911 kb on - strand at 925.098 kb on - strand at 925.166 kb on - strand at 925.241 kb on - strand at 925.242 kb on + strand at 925.249 kb on + strand at 925.272 kb on - strand at 925.273 kb on + strand at 925.349 kb on - strand at 925.558 kb on + strand at 925.561 kb on - strand at 925.562 kb on + strand at 925.598 kb on - strand at 925.599 kb on + strand at 925.630 kb on + strand at 925.701 kb on + strand at 925.755 kb on - strand at 925.755 kb on - strand at 925.932 kb on - strand at 925.933 kb on + strand at 925.938 kb on + strand at 925.951 kb on + strand at 926.001 kb on - strand at 926.002 kb on + strand at 926.022 kb on - strand at 926.045 kb on + strand at 926.057 kb on - strand at 926.088 kb on - strand at 926.145 kb on + strand at 926.145 kb on + strand at 926.147 kb on + strand at 926.148 kb on - strand at 926.213 kb on - strand at 926.359 kb on + strand at 926.384 kb on - strand at 926.385 kb on + strand at 926.393 kb on - strand at 926.417 kb on - strand at 926.431 kb on - strand at 926.432 kb on + strand at 926.440 kb on + strand at 926.440 kb on + strand at 926.440 kb on + strand at 926.517 kb on + strand, within MMP_RS04870 at 926.568 kb on + strand, within MMP_RS04870 at 926.589 kb on + strand, within MMP_RS04870 at 926.620 kb on - strand, within MMP_RS04870 at 926.621 kb on + strand, within MMP_RS04870 at 926.635 kb on + strand, within MMP_RS04870 at 926.636 kb on - strand, within MMP_RS04870 at 926.637 kb on + strand, within MMP_RS04870 at 926.808 kb on + strand, within MMP_RS04870 at 926.912 kb on - strand, within MMP_RS04870 at 927.073 kb on - strand at 927.073 kb on - strand at 927.073 kb on - strand at 927.074 kb on + strand at 927.076 kb on + strand at 927.083 kb on + strand at 927.118 kb on + strand, within MMP_RS04875 at 927.158 kb on + strand, within MMP_RS04875 at 927.294 kb on + strand, within MMP_RS04875 at 927.313 kb on - strand, within MMP_RS04875 at 927.314 kb on + strand, within MMP_RS04875 at 927.317 kb on - strand, within MMP_RS04875 at 927.318 kb on + strand, within MMP_RS04875 at 927.318 kb on + strand, within MMP_RS04875 at 927.352 kb on - strand at 927.353 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 924,683 + -2.8 924,725 + -2.9 924,756 + -2.3 924,819 + -2.8 924,876 - -0.5 924,908 - -3.5 924,911 - -2.2 925,098 - -3.8 925,166 - -2.4 925,241 - -2.4 925,242 + -2.4 925,249 + -0.7 925,272 - -1.3 925,273 + -5.3 925,349 - -3.2 925,558 + +0.4 925,561 - +0.2 925,562 + -0.3 925,598 - -1.1 925,599 + -1.0 925,630 + -0.4 925,701 + -3.6 925,755 - -2.8 925,755 - -3.6 925,932 - -3.2 925,933 + -1.3 925,938 + -1.6 925,951 + -2.3 926,001 - -0.4 926,002 + -2.2 926,022 - -0.6 926,045 + -1.2 926,057 - -1.6 926,088 - -3.8 926,145 + -3.0 926,145 + -1.9 926,147 + -2.0 926,148 - -2.1 926,213 - -1.9 926,359 + -3.7 926,384 - -0.5 926,385 + -2.6 926,393 - +0.1 926,417 - -0.6 926,431 - -1.9 926,432 + -2.4 926,440 + -2.6 926,440 + -5.5 926,440 + -1.6 926,517 + MMP_RS04870 0.20 -1.7 926,568 + MMP_RS04870 0.28 -2.5 926,589 + MMP_RS04870 0.31 -1.6 926,620 - MMP_RS04870 0.36 -3.6 926,621 + MMP_RS04870 0.36 -2.7 926,635 + MMP_RS04870 0.38 -2.3 926,636 - MMP_RS04870 0.38 -1.8 926,637 + MMP_RS04870 0.38 -3.6 926,808 + MMP_RS04870 0.63 -3.4 926,912 - MMP_RS04870 0.79 -2.9 927,073 - -4.3 927,073 - -3.3 927,073 - -2.3 927,074 + -3.5 927,076 + -2.2 927,083 + -3.0 927,118 + MMP_RS04875 0.14 -0.7 927,158 + MMP_RS04875 0.27 -1.0 927,294 + MMP_RS04875 0.75 -0.9 927,313 - MMP_RS04875 0.81 -2.7 927,314 + MMP_RS04875 0.82 -3.0 927,317 - MMP_RS04875 0.83 -1.2 927,318 + MMP_RS04875 0.83 -2.1 927,318 + MMP_RS04875 0.83 -1.5 927,352 - -1.8 927,353 + -1.9
Or see this region's nucleotide sequence