Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS04630 and MMP_RS04635 are separated by 23 nucleotides MMP_RS04635 and MMP_RS04640 are separated by 163 nucleotides MMP_RS04640 and MMP_RS04645 are separated by 22 nucleotides
MMP_RS04630: MMP_RS04630 - 3'-5' exonuclease, at 884,248 to 884,823
_RS04630
MMP_RS04635: MMP_RS04635 - GNAT family N-acetyltransferase, at 884,847 to 885,395
_RS04635
MMP_RS04640: MMP_RS04640 - CTP synthase (glutamine hydrolyzing), at 885,559 to 887,160
_RS04640
MMP_RS04645: MMP_RS04645 - glutamine-hydrolyzing GMP synthase, at 887,183 to 888,115
_RS04645
Position (kb) 885
886
887
888 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 884.581 kb on + strand, within MMP_RS04630 at 884.581 kb on + strand, within MMP_RS04630 at 884.638 kb on - strand, within MMP_RS04630 at 884.641 kb on + strand, within MMP_RS04630 at 884.756 kb on + strand, within MMP_RS04630 at 884.769 kb on + strand at 884.771 kb on - strand at 884.771 kb on - strand at 884.771 kb on - strand at 884.773 kb on - strand at 884.774 kb on + strand at 884.810 kb on - strand at 884.811 kb on + strand at 884.837 kb on + strand at 885.129 kb on - strand, within MMP_RS04635 at 885.177 kb on + strand, within MMP_RS04635 at 885.177 kb on + strand, within MMP_RS04635 at 885.178 kb on - strand, within MMP_RS04635 at 885.183 kb on + strand, within MMP_RS04635 at 885.185 kb on - strand, within MMP_RS04635 at 885.191 kb on - strand, within MMP_RS04635 at 885.191 kb on - strand, within MMP_RS04635 at 885.248 kb on - strand, within MMP_RS04635 at 885.274 kb on + strand, within MMP_RS04635 at 885.351 kb on - strand at 885.352 kb on + strand at 885.352 kb on + strand at 885.360 kb on - strand at 885.378 kb on - strand at 885.378 kb on - strand at 885.378 kb on - strand at 885.384 kb on + strand at 885.395 kb on - strand at 885.396 kb on + strand at 885.396 kb on + strand at 885.416 kb on - strand at 885.417 kb on + strand at 885.425 kb on + strand at 885.426 kb on - strand at 885.426 kb on - strand at 885.427 kb on + strand at 885.427 kb on + strand at 885.427 kb on + strand at 885.439 kb on - strand at 885.439 kb on - strand at 885.440 kb on + strand at 885.440 kb on + strand at 885.440 kb on + strand at 885.440 kb on + strand at 885.452 kb on - strand at 885.452 kb on - strand at 885.512 kb on + strand at 885.512 kb on + strand at 885.540 kb on - strand at 885.566 kb on + strand at 885.568 kb on + strand at 885.569 kb on - strand at 885.570 kb on + strand at 885.588 kb on + strand at 886.089 kb on - strand at 886.170 kb on - strand at 886.182 kb on - strand at 886.349 kb on - strand at 886.474 kb on + strand at 887.953 kb on - strand at 888.152 kb on + strand at 888.155 kb on - strand at 888.156 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 884,581 + MMP_RS04630 0.58 +0.3 884,581 + MMP_RS04630 0.58 -1.9 884,638 - MMP_RS04630 0.68 -0.8 884,641 + MMP_RS04630 0.68 -0.5 884,756 + MMP_RS04630 0.88 -0.3 884,769 + -1.0 884,771 - +0.1 884,771 - -0.2 884,771 - -0.5 884,773 - +0.0 884,774 + +0.4 884,810 - +0.0 884,811 + -1.2 884,837 + +0.1 885,129 - MMP_RS04635 0.51 +1.1 885,177 + MMP_RS04635 0.60 -0.2 885,177 + MMP_RS04635 0.60 +0.5 885,178 - MMP_RS04635 0.60 +1.4 885,183 + MMP_RS04635 0.61 +0.8 885,185 - MMP_RS04635 0.62 +0.6 885,191 - MMP_RS04635 0.63 -0.1 885,191 - MMP_RS04635 0.63 -0.2 885,248 - MMP_RS04635 0.73 +0.5 885,274 + MMP_RS04635 0.78 +0.9 885,351 - +1.0 885,352 + +0.9 885,352 + -0.6 885,360 - -3.2 885,378 - +0.3 885,378 - +0.3 885,378 - -0.1 885,384 + +0.6 885,395 - +0.4 885,396 + +0.5 885,396 + +0.1 885,416 - -0.8 885,417 + +0.6 885,425 + -0.3 885,426 - -0.8 885,426 - +0.5 885,427 + +0.7 885,427 + +0.3 885,427 + -0.7 885,439 - -0.2 885,439 - +0.2 885,440 + -1.6 885,440 + +0.9 885,440 + +0.7 885,440 + -0.9 885,452 - +0.2 885,452 - -0.3 885,512 + +0.8 885,512 + -3.9 885,540 - -2.8 885,566 + -1.1 885,568 + -4.3 885,569 - -2.8 885,570 + -3.5 885,588 + -3.5 886,089 - -0.8 886,170 - -0.2 886,182 - -3.5 886,349 - -1.2 886,474 + -3.8 887,953 - -1.3 888,152 + +0.0 888,155 - +0.8 888,156 + +0.7
Or see this region's nucleotide sequence