Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03845 and MMP_RS08990 are separated by 27 nucleotides MMP_RS08990 and MMP_RS03850 are separated by 125 nucleotides MMP_RS03850 and MMP_RS03855 are separated by 24 nucleotides MMP_RS03855 and MMP_RS03860 are separated by 81 nucleotides
MMP_RS03845: MMP_RS03845 - DNA adenine methylase, at 727,879 to 728,799
_RS03845
MMP_RS08990: MMP_RS08990 - hypothetical protein, at 728,827 to 728,997
_RS08990
MMP_RS03850: MMP_RS03850 - PRC-barrel domain-containing protein, at 729,123 to 729,377
_RS03850
MMP_RS03855: MMP_RS03855 - aspartate dehydrogenase, at 729,402 to 730,205
_RS03855
MMP_RS03860: MMP_RS03860 - hypothetical protein, at 730,287 to 731,216
_RS03860
Position (kb) 729
730
731 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 728.472 kb on + strand, within MMP_RS03845 at 728.583 kb on + strand, within MMP_RS03845 at 728.602 kb on + strand, within MMP_RS03845 at 728.626 kb on - strand, within MMP_RS03845 at 728.627 kb on + strand, within MMP_RS03845 at 728.627 kb on + strand, within MMP_RS03845 at 728.640 kb on - strand, within MMP_RS03845 at 728.641 kb on + strand, within MMP_RS03845 at 728.658 kb on - strand, within MMP_RS03845 at 728.704 kb on + strand, within MMP_RS03845 at 728.777 kb on - strand at 728.823 kb on - strand at 728.823 kb on - strand at 728.823 kb on - strand at 728.824 kb on + strand at 728.951 kb on + strand, within MMP_RS08990 at 728.952 kb on - strand, within MMP_RS08990 at 728.952 kb on - strand, within MMP_RS08990 at 728.953 kb on + strand, within MMP_RS08990 at 728.984 kb on - strand at 728.985 kb on + strand at 728.985 kb on + strand at 728.985 kb on + strand at 728.997 kb on - strand at 728.997 kb on - strand at 728.998 kb on + strand at 728.998 kb on + strand at 728.998 kb on + strand at 729.003 kb on + strand at 729.033 kb on - strand at 729.034 kb on + strand at 729.042 kb on - strand at 729.042 kb on - strand at 729.042 kb on - strand at 729.047 kb on - strand at 729.047 kb on - strand at 729.048 kb on + strand at 729.070 kb on - strand at 729.076 kb on + strand at 729.080 kb on - strand at 729.089 kb on - strand at 729.120 kb on + strand at 729.120 kb on + strand at 729.168 kb on + strand at 729.178 kb on - strand at 729.180 kb on - strand at 729.180 kb on - strand at 729.181 kb on + strand at 729.181 kb on + strand at 729.219 kb on + strand at 729.384 kb on + strand at 729.387 kb on - strand at 729.388 kb on + strand at 729.419 kb on + strand at 729.755 kb on + strand at 729.760 kb on - strand at 729.986 kb on + strand at 730.115 kb on - strand at 730.271 kb on + strand at 730.288 kb on - strand at 730.288 kb on - strand at 730.288 kb on - strand at 730.289 kb on + strand at 730.343 kb on - strand at 730.393 kb on + strand, within MMP_RS03860 at 730.429 kb on + strand, within MMP_RS03860 at 730.481 kb on - strand, within MMP_RS03860 at 730.645 kb on + strand, within MMP_RS03860 at 730.845 kb on - strand, within MMP_RS03860 at 730.845 kb on - strand, within MMP_RS03860 at 730.849 kb on - strand, within MMP_RS03860 at 730.849 kb on - strand, within MMP_RS03860 at 730.886 kb on - strand, within MMP_RS03860 at 730.895 kb on - strand, within MMP_RS03860 at 730.935 kb on - strand, within MMP_RS03860 at 730.935 kb on - strand, within MMP_RS03860 at 730.935 kb on - strand, within MMP_RS03860 at 730.936 kb on + strand, within MMP_RS03860 at 730.968 kb on - strand, within MMP_RS03860 at 730.973 kb on - strand, within MMP_RS03860 at 730.974 kb on + strand, within MMP_RS03860 at 730.974 kb on + strand, within MMP_RS03860 at 731.002 kb on + strand, within MMP_RS03860 at 731.036 kb on - strand, within MMP_RS03860 at 731.081 kb on - strand, within MMP_RS03860 at 731.082 kb on + strand, within MMP_RS03860 at 731.132 kb on - strand at 731.132 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 728,472 + MMP_RS03845 0.64 +0.2 728,583 + MMP_RS03845 0.76 -0.5 728,602 + MMP_RS03845 0.79 +0.6 728,626 - MMP_RS03845 0.81 -1.5 728,627 + MMP_RS03845 0.81 +1.1 728,627 + MMP_RS03845 0.81 +0.2 728,640 - MMP_RS03845 0.83 +0.3 728,641 + MMP_RS03845 0.83 -0.6 728,658 - MMP_RS03845 0.85 +0.2 728,704 + MMP_RS03845 0.90 +0.3 728,777 - -1.2 728,823 - +0.6 728,823 - +0.4 728,823 - +0.3 728,824 + +0.4 728,951 + MMP_RS08990 0.73 +0.5 728,952 - MMP_RS08990 0.73 +1.1 728,952 - MMP_RS08990 0.73 -0.8 728,953 + MMP_RS08990 0.74 +0.3 728,984 - +0.2 728,985 + +0.2 728,985 + -0.3 728,985 + +0.6 728,997 - +0.8 728,997 - -0.9 728,998 + +0.2 728,998 + +0.2 728,998 + +0.9 729,003 + -0.1 729,033 - -0.6 729,034 + +0.1 729,042 - +0.7 729,042 - +0.6 729,042 - -1.0 729,047 - -0.2 729,047 - -0.4 729,048 + -0.9 729,070 - -2.8 729,076 + -2.3 729,080 - -2.7 729,089 - -3.0 729,120 + -1.9 729,120 + -3.3 729,168 + -4.3 729,178 - -3.8 729,180 - -3.4 729,180 - -3.6 729,181 + -2.7 729,181 + -2.9 729,219 + -3.5 729,384 + -4.2 729,387 - -1.3 729,388 + -3.1 729,419 + -3.0 729,755 + -1.6 729,760 - -2.5 729,986 + -3.1 730,115 - -3.0 730,271 + +0.7 730,288 - +0.5 730,288 - +0.5 730,288 - +0.3 730,289 + -0.4 730,343 - +0.6 730,393 + MMP_RS03860 0.11 -1.0 730,429 + MMP_RS03860 0.15 -0.8 730,481 - MMP_RS03860 0.21 -0.2 730,645 + MMP_RS03860 0.38 +0.8 730,845 - MMP_RS03860 0.60 +0.8 730,845 - MMP_RS03860 0.60 -0.8 730,849 - MMP_RS03860 0.60 -0.6 730,849 - MMP_RS03860 0.60 +0.9 730,886 - MMP_RS03860 0.64 +0.7 730,895 - MMP_RS03860 0.65 +0.7 730,935 - MMP_RS03860 0.70 -0.6 730,935 - MMP_RS03860 0.70 +0.3 730,935 - MMP_RS03860 0.70 -0.0 730,936 + MMP_RS03860 0.70 +0.4 730,968 - MMP_RS03860 0.73 -0.8 730,973 - MMP_RS03860 0.74 -0.1 730,974 + MMP_RS03860 0.74 +0.3 730,974 + MMP_RS03860 0.74 -1.1 731,002 + MMP_RS03860 0.77 -0.3 731,036 - MMP_RS03860 0.81 +0.8 731,081 - MMP_RS03860 0.85 -0.1 731,082 + MMP_RS03860 0.85 +0.2 731,132 - -0.1 731,132 - +0.6
Or see this region's nucleotide sequence