Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03595 and MMP_RS03600 are separated by 78 nucleotides MMP_RS03600 and MMP_RS03605 are separated by 53 nucleotides MMP_RS03605 and MMP_RS03610 are separated by 94 nucleotides
MMP_RS03595: MMP_RS03595 - 2-amino-3 7-dideoxy-D-threo-hept-6-ulosonate synthase, at 674,140 to 674,958
_RS03595
MMP_RS03600: MMP_RS03600 - triose-phosphate isomerase, at 675,037 to 675,702
_RS03600
MMP_RS03605: MMP_RS03605 - O-phosphoserine--tRNA ligase, at 675,756 to 677,369
_RS03605
MMP_RS03610: MMP_RS03610 - NCS2 family permease, at 677,464 to 678,765
_RS03610
Position (kb)
675
676
677
678 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 674.887 kb on + strand at 674.948 kb on - strand at 674.985 kb on - strand at 674.992 kb on + strand at 675.013 kb on + strand at 675.015 kb on - strand at 675.258 kb on - strand, within MMP_RS03600 at 675.259 kb on + strand, within MMP_RS03600 at 675.259 kb on + strand, within MMP_RS03600 at 675.561 kb on + strand, within MMP_RS03600 at 675.579 kb on + strand, within MMP_RS03600 at 675.700 kb on - strand at 675.781 kb on + strand at 676.012 kb on + strand at 676.245 kb on - strand at 676.557 kb on + strand at 676.684 kb on - strand at 676.936 kb on + strand at 677.133 kb on + strand at 677.439 kb on - strand at 677.439 kb on - strand at 677.440 kb on + strand at 677.440 kb on + strand at 677.485 kb on - strand at 677.485 kb on - strand at 677.485 kb on - strand at 677.486 kb on + strand at 677.541 kb on - strand at 677.541 kb on - strand at 677.541 kb on - strand at 677.564 kb on + strand at 677.564 kb on + strand at 677.564 kb on + strand at 677.570 kb on - strand at 677.637 kb on - strand, within MMP_RS03610 at 677.637 kb on - strand, within MMP_RS03610 at 677.637 kb on - strand, within MMP_RS03610 at 677.637 kb on - strand, within MMP_RS03610 at 677.637 kb on - strand, within MMP_RS03610 at 677.638 kb on + strand, within MMP_RS03610 at 677.661 kb on - strand, within MMP_RS03610 at 677.679 kb on - strand, within MMP_RS03610 at 677.679 kb on - strand, within MMP_RS03610 at 677.680 kb on + strand, within MMP_RS03610 at 677.739 kb on + strand, within MMP_RS03610 at 677.797 kb on + strand, within MMP_RS03610 at 677.840 kb on - strand, within MMP_RS03610 at 677.874 kb on - strand, within MMP_RS03610 at 677.883 kb on + strand, within MMP_RS03610 at 677.883 kb on + strand, within MMP_RS03610 at 677.949 kb on - strand, within MMP_RS03610 at 677.950 kb on + strand, within MMP_RS03610 at 677.950 kb on + strand, within MMP_RS03610 at 677.950 kb on + strand, within MMP_RS03610 at 677.958 kb on + strand, within MMP_RS03610 at 678.183 kb on - strand, within MMP_RS03610 at 678.198 kb on + strand, within MMP_RS03610 at 678.231 kb on + strand, within MMP_RS03610 at 678.237 kb on - strand, within MMP_RS03610 at 678.317 kb on - strand, within MMP_RS03610 at 678.318 kb on + strand, within MMP_RS03610 at 678.361 kb on + strand, within MMP_RS03610 at 678.361 kb on + strand, within MMP_RS03610
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 674,887 + -0.8 674,948 - -0.3 674,985 - +1.4 674,992 + -0.2 675,013 + -4.1 675,015 - -2.1 675,258 - MMP_RS03600 0.33 +0.0 675,259 + MMP_RS03600 0.33 -3.7 675,259 + MMP_RS03600 0.33 -2.7 675,561 + MMP_RS03600 0.79 -3.9 675,579 + MMP_RS03600 0.81 +0.8 675,700 - -0.4 675,781 + -3.2 676,012 + -2.3 676,245 - -2.8 676,557 + -3.0 676,684 - -3.8 676,936 + -3.4 677,133 + -2.5 677,439 - +0.4 677,439 - +0.6 677,440 + -1.4 677,440 + -3.6 677,485 - -0.3 677,485 - -0.4 677,485 - -0.3 677,486 + -0.8 677,541 - +0.2 677,541 - -0.2 677,541 - -0.9 677,564 + -1.1 677,564 + +0.5 677,564 + +0.2 677,570 - -0.1 677,637 - MMP_RS03610 0.13 -0.3 677,637 - MMP_RS03610 0.13 -0.9 677,637 - MMP_RS03610 0.13 -0.1 677,637 - MMP_RS03610 0.13 -0.0 677,637 - MMP_RS03610 0.13 +0.3 677,638 + MMP_RS03610 0.13 -1.0 677,661 - MMP_RS03610 0.15 -0.2 677,679 - MMP_RS03610 0.17 -0.2 677,679 - MMP_RS03610 0.17 +0.6 677,680 + MMP_RS03610 0.17 +0.6 677,739 + MMP_RS03610 0.21 +0.0 677,797 + MMP_RS03610 0.26 +0.3 677,840 - MMP_RS03610 0.29 +0.5 677,874 - MMP_RS03610 0.31 +0.3 677,883 + MMP_RS03610 0.32 +0.3 677,883 + MMP_RS03610 0.32 +0.2 677,949 - MMP_RS03610 0.37 +0.1 677,950 + MMP_RS03610 0.37 -0.2 677,950 + MMP_RS03610 0.37 +0.2 677,950 + MMP_RS03610 0.37 -0.5 677,958 + MMP_RS03610 0.38 -0.7 678,183 - MMP_RS03610 0.55 +0.3 678,198 + MMP_RS03610 0.56 -1.2 678,231 + MMP_RS03610 0.59 -1.6 678,237 - MMP_RS03610 0.59 -0.6 678,317 - MMP_RS03610 0.66 +0.3 678,318 + MMP_RS03610 0.66 -2.0 678,361 + MMP_RS03610 0.69 -0.2 678,361 + MMP_RS03610 0.69 -0.1
Or see this region's nucleotide sequence