Strain Fitness in Methanococcus maripaludis S2 around MMP_RS03185

Experiment: H2 N-free with 10mM L-Aspartic Acid

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMP_RS03175 and MMP_RS03180 are separated by 8 nucleotidesMMP_RS03180 and MMP_RS03185 are separated by 80 nucleotidesMMP_RS03185 and MMP_RS03190 are separated by 53 nucleotidesMMP_RS03190 and MMP_RS03195 are separated by 24 nucleotides MMP_RS03175: MMP_RS03175 - translation initiation factor eIF-1A, at 598,821 to 599,135 _RS03175 MMP_RS03180: MMP_RS03180 - serine protein kinase RIO, at 599,144 to 599,965 _RS03180 MMP_RS03185: MMP_RS03185 - KH domain-containing protein, at 600,046 to 600,600 _RS03185 MMP_RS03190: MMP_RS03190 - RlmE family RNA methyltransferase, at 600,654 to 601,430 _RS03190 MMP_RS03195: MMP_RS03195 - global nitrogen regulator NrpR, at 601,455 to 603,065 _RS03195 Position (kb) 600 601Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 599.116 kb on + strandat 599.499 kb on - strandat 599.527 kb on - strandat 599.701 kb on + strandat 599.777 kb on - strandat 600.004 kb on + strandat 600.066 kb on + strandat 600.417 kb on + strandat 600.632 kb on - strandat 600.744 kb on + strandat 600.781 kb on + strandat 600.841 kb on - strandat 600.842 kb on + strandat 601.029 kb on - strandat 601.279 kb on + strandat 601.300 kb on + strandat 601.320 kb on - strandat 601.337 kb on + strandat 601.337 kb on + strandat 601.442 kb on - strandat 601.443 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid
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599,116 + -2.6
599,499 - -0.8
599,527 - -1.4
599,701 + -1.1
599,777 - -2.2
600,004 + -2.1
600,066 + +0.2
600,417 + -0.1
600,632 - -0.2
600,744 + -4.3
600,781 + -2.0
600,841 - -1.8
600,842 + -1.8
601,029 - -2.2
601,279 + -3.3
601,300 + -2.2
601,320 - +0.5
601,337 + -3.1
601,337 + -2.2
601,442 - -1.8
601,443 + -1.8

Or see this region's nucleotide sequence