Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03125 and MMP_RS03130 are separated by 76 nucleotides MMP_RS03130 and MMP_RS03135 are separated by 18 nucleotides MMP_RS03135 and MMP_RS03140 are separated by 193 nucleotides
MMP_RS03125: MMP_RS03125 - cell division ATPase MinD, at 588,917 to 589,702
_RS03125
MMP_RS03130: MMP_RS03130 - hypothetical protein, at 589,779 to 590,345
_RS03130
MMP_RS03135: MMP_RS03135 - O-phosphoseryl-tRNA(Sec) selenium transferase, at 590,364 to 591,674
_RS03135
MMP_RS03140: MMP_RS03140 - hypothetical protein, at 591,868 to 593,298
_RS03140
Position (kb) 590
591
592 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 589.596 kb on + strand, within MMP_RS03125 at 589.683 kb on + strand at 589.736 kb on - strand at 589.737 kb on + strand at 589.737 kb on + strand at 589.738 kb on - strand at 589.738 kb on - strand at 589.738 kb on - strand at 589.739 kb on + strand at 589.739 kb on + strand at 589.739 kb on + strand at 589.739 kb on + strand at 589.739 kb on + strand at 589.740 kb on - strand at 589.741 kb on + strand at 589.746 kb on + strand at 589.754 kb on + strand at 589.764 kb on - strand at 589.767 kb on - strand at 589.767 kb on - strand at 589.767 kb on - strand at 589.767 kb on - strand at 589.768 kb on + strand at 589.769 kb on - strand at 589.769 kb on - strand at 589.780 kb on - strand at 589.827 kb on + strand at 589.827 kb on + strand at 589.887 kb on + strand, within MMP_RS03130 at 589.889 kb on - strand, within MMP_RS03130 at 589.897 kb on + strand, within MMP_RS03130 at 590.016 kb on + strand, within MMP_RS03130 at 590.031 kb on - strand, within MMP_RS03130 at 590.055 kb on - strand, within MMP_RS03130 at 590.060 kb on - strand, within MMP_RS03130 at 590.100 kb on + strand, within MMP_RS03130 at 590.100 kb on + strand, within MMP_RS03130 at 590.103 kb on + strand, within MMP_RS03130 at 590.267 kb on - strand, within MMP_RS03130 at 590.277 kb on - strand, within MMP_RS03130 at 590.323 kb on - strand at 590.387 kb on - strand at 590.401 kb on + strand at 590.446 kb on - strand at 590.528 kb on - strand at 590.730 kb on + strand at 590.905 kb on - strand at 590.983 kb on + strand at 591.114 kb on - strand at 591.321 kb on - strand at 591.340 kb on - strand at 591.341 kb on + strand at 591.341 kb on + strand at 591.347 kb on - strand at 591.446 kb on + strand at 591.531 kb on - strand at 591.715 kb on - strand at 591.750 kb on - strand at 591.779 kb on - strand at 591.782 kb on - strand at 591.783 kb on + strand at 591.802 kb on - strand at 591.824 kb on - strand at 591.831 kb on + strand at 591.956 kb on + strand at 591.997 kb on - strand at 591.998 kb on + strand at 592.086 kb on - strand, within MMP_RS03140 at 592.249 kb on + strand, within MMP_RS03140 at 592.256 kb on - strand, within MMP_RS03140 at 592.256 kb on - strand, within MMP_RS03140 at 592.338 kb on + strand, within MMP_RS03140 at 592.339 kb on - strand, within MMP_RS03140 at 592.340 kb on + strand, within MMP_RS03140 at 592.377 kb on - strand, within MMP_RS03140 at 592.381 kb on - strand, within MMP_RS03140 at 592.382 kb on + strand, within MMP_RS03140 at 592.420 kb on - strand, within MMP_RS03140 at 592.423 kb on + strand, within MMP_RS03140 at 592.554 kb on + strand, within MMP_RS03140 at 592.564 kb on - strand, within MMP_RS03140 at 592.565 kb on + strand, within MMP_RS03140 at 592.571 kb on + strand, within MMP_RS03140 at 592.667 kb on - strand, within MMP_RS03140
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 589,596 + MMP_RS03125 0.86 +0.8 589,683 + -1.0 589,736 - +0.0 589,737 + -0.1 589,737 + -0.0 589,738 - -0.1 589,738 - -0.2 589,738 - +0.7 589,739 + +0.5 589,739 + -0.4 589,739 + +1.6 589,739 + -0.4 589,739 + -0.1 589,740 - -0.2 589,741 + +0.6 589,746 + +0.8 589,754 + +0.9 589,764 - -0.1 589,767 - +0.4 589,767 - -1.5 589,767 - +0.6 589,767 - -0.2 589,768 + +0.1 589,769 - -0.1 589,769 - +0.8 589,780 - +1.1 589,827 + +0.1 589,827 + +0.8 589,887 + MMP_RS03130 0.19 -0.5 589,889 - MMP_RS03130 0.19 +0.6 589,897 + MMP_RS03130 0.21 +0.1 590,016 + MMP_RS03130 0.42 +0.1 590,031 - MMP_RS03130 0.44 +1.3 590,055 - MMP_RS03130 0.49 +0.8 590,060 - MMP_RS03130 0.50 +0.9 590,100 + MMP_RS03130 0.57 +0.3 590,100 + MMP_RS03130 0.57 +0.2 590,103 + MMP_RS03130 0.57 -0.5 590,267 - MMP_RS03130 0.86 -0.1 590,277 - MMP_RS03130 0.88 +0.2 590,323 - +0.9 590,387 - -1.6 590,401 + -2.2 590,446 - -3.0 590,528 - -2.8 590,730 + -2.0 590,905 - -4.1 590,983 + -2.8 591,114 - -2.6 591,321 - -4.9 591,340 - -3.8 591,341 + -1.4 591,341 + -3.6 591,347 - -3.9 591,446 + -1.9 591,531 - -2.4 591,715 - +0.6 591,750 - -0.2 591,779 - +0.9 591,782 - +0.5 591,783 + -0.7 591,802 - -0.8 591,824 - -1.5 591,831 + -0.2 591,956 + -2.4 591,997 - -1.1 591,998 + -0.4 592,086 - MMP_RS03140 0.15 -1.4 592,249 + MMP_RS03140 0.27 +0.1 592,256 - MMP_RS03140 0.27 -1.8 592,256 - MMP_RS03140 0.27 -1.2 592,338 + MMP_RS03140 0.33 -1.8 592,339 - MMP_RS03140 0.33 -0.6 592,340 + MMP_RS03140 0.33 -1.6 592,377 - MMP_RS03140 0.36 -1.0 592,381 - MMP_RS03140 0.36 -0.9 592,382 + MMP_RS03140 0.36 -1.8 592,420 - MMP_RS03140 0.39 -0.8 592,423 + MMP_RS03140 0.39 -0.7 592,554 + MMP_RS03140 0.48 -0.6 592,564 - MMP_RS03140 0.49 +0.2 592,565 + MMP_RS03140 0.49 -0.5 592,571 + MMP_RS03140 0.49 -0.4 592,667 - MMP_RS03140 0.56 -0.8
Or see this region's nucleotide sequence