Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03065 and MMP_RS03070 overlap by 8 nucleotides MMP_RS03070 and MMP_RS03075 are separated by 52 nucleotides MMP_RS03075 and MMP_RS03080 are separated by 422 nucleotides MMP_RS03080 and MMP_RS03085 are separated by 4 nucleotides
MMP_RS03065: MMP_RS03065 - AzlC family ABC transporter permease, at 577,959 to 578,675
_RS03065
MMP_RS03070: MMP_RS03070 - AzlD domain-containing protein, at 578,668 to 578,976
_RS03070
MMP_RS03075: MMP_RS03075 - valine--tRNA ligase, at 579,029 to 581,689
_RS03075
MMP_RS03080: MMP_RS03080 - universal stress protein, at 582,112 to 582,549
_RS03080
MMP_RS03085: MMP_RS03085 - endonuclease III, at 582,554 to 583,252
_RS03085
Position (kb) 579
580
581
582 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 578.099 kb on + strand, within MMP_RS03065 at 578.179 kb on - strand, within MMP_RS03065 at 578.621 kb on - strand at 578.621 kb on - strand at 578.622 kb on + strand at 578.653 kb on - strand at 578.690 kb on - strand at 578.691 kb on + strand at 578.691 kb on + strand at 578.719 kb on - strand, within MMP_RS03070 at 578.719 kb on - strand, within MMP_RS03070 at 578.720 kb on + strand, within MMP_RS03070 at 578.720 kb on + strand, within MMP_RS03070 at 578.720 kb on + strand, within MMP_RS03070 at 578.861 kb on + strand, within MMP_RS03070 at 578.903 kb on + strand, within MMP_RS03070 at 578.907 kb on - strand, within MMP_RS03070 at 578.953 kb on - strand at 578.953 kb on - strand at 578.954 kb on + strand at 578.954 kb on + strand at 578.955 kb on - strand at 578.956 kb on + strand at 579.273 kb on + strand at 579.320 kb on - strand at 580.676 kb on - strand at 581.269 kb on - strand at 581.272 kb on - strand at 581.274 kb on - strand at 581.786 kb on - strand at 581.786 kb on - strand at 581.786 kb on - strand at 581.795 kb on - strand at 581.871 kb on + strand at 581.923 kb on + strand at 581.938 kb on - strand at 581.959 kb on + strand at 581.963 kb on + strand at 581.963 kb on + strand at 581.975 kb on - strand at 582.008 kb on - strand at 582.059 kb on + strand at 582.059 kb on + strand at 582.064 kb on - strand at 582.064 kb on - strand at 582.066 kb on - strand at 582.066 kb on - strand at 582.067 kb on + strand at 582.068 kb on - strand at 582.069 kb on + strand at 582.080 kb on - strand at 582.359 kb on - strand, within MMP_RS03080 at 582.360 kb on + strand, within MMP_RS03080 at 582.476 kb on - strand, within MMP_RS03080 at 582.477 kb on + strand, within MMP_RS03080 at 582.555 kb on - strand at 582.556 kb on + strand at 582.584 kb on - strand at 582.585 kb on + strand at 582.589 kb on + strand at 582.654 kb on + strand, within MMP_RS03085
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 578,099 + MMP_RS03065 0.20 +1.2 578,179 - MMP_RS03065 0.31 -0.1 578,621 - +0.9 578,621 - +0.4 578,622 + -0.3 578,653 - +0.4 578,690 - +0.3 578,691 + +1.2 578,691 + +0.5 578,719 - MMP_RS03070 0.17 -0.3 578,719 - MMP_RS03070 0.17 +0.1 578,720 + MMP_RS03070 0.17 +0.2 578,720 + MMP_RS03070 0.17 -0.4 578,720 + MMP_RS03070 0.17 +0.4 578,861 + MMP_RS03070 0.62 -2.6 578,903 + MMP_RS03070 0.76 +0.2 578,907 - MMP_RS03070 0.77 +0.5 578,953 - +0.2 578,953 - -0.6 578,954 + +0.2 578,954 + +0.2 578,955 - +1.1 578,956 + +0.1 579,273 + -4.5 579,320 - -2.2 580,676 - -0.8 581,269 - -3.4 581,272 - -2.4 581,274 - -2.7 581,786 - +0.1 581,786 - -0.6 581,786 - -0.3 581,795 - +1.1 581,871 + -0.2 581,923 + +0.2 581,938 - +0.2 581,959 + +0.1 581,963 + +1.0 581,963 + -0.6 581,975 - -0.1 582,008 - -0.3 582,059 + -0.2 582,059 + -0.2 582,064 - +0.6 582,064 - +0.4 582,066 - -0.3 582,066 - +0.3 582,067 + +0.3 582,068 - -0.2 582,069 + -0.9 582,080 - +0.1 582,359 - MMP_RS03080 0.56 +0.1 582,360 + MMP_RS03080 0.57 -1.5 582,476 - MMP_RS03080 0.83 -2.8 582,477 + MMP_RS03080 0.83 -0.6 582,555 - +1.0 582,556 + +0.7 582,584 - +0.0 582,585 + +0.2 582,589 + -1.2 582,654 + MMP_RS03085 0.14 +1.3
Or see this region's nucleotide sequence