Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02855 and MMP_RS02860 are separated by 98 nucleotides MMP_RS02860 and MMP_RS02865 are separated by 36 nucleotides MMP_RS02865 and MMP_RS02870 are separated by 125 nucleotides MMP_RS02870 and MMP_RS02875 are separated by 33 nucleotides
MMP_RS02855: MMP_RS02855 - 3-isopropylmalate dehydrogenase, at 543,913 to 544,905
_RS02855
MMP_RS02860: MMP_RS02860 - phosphoribosylaminoimidazolesuccinocarboxamide synthase, at 545,004 to 545,744
_RS02860
MMP_RS02865: MMP_RS02865 - phosphoserine phosphatase SerB, at 545,781 to 546,422
_RS02865
MMP_RS02870: MMP_RS02870 - hypothetical protein, at 546,548 to 547,150
_RS02870
MMP_RS02875: MMP_RS02875 - chorismate pyruvate-lyase family protein, at 547,184 to 547,699
_RS02875
Position (kb) 545
546
547 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 544.923 kb on + strand at 544.945 kb on + strand at 544.951 kb on - strand at 544.952 kb on + strand at 544.952 kb on + strand at 544.964 kb on - strand at 544.965 kb on + strand at 545.248 kb on + strand at 545.430 kb on + strand at 545.612 kb on + strand at 546.064 kb on + strand at 546.306 kb on + strand at 546.498 kb on + strand at 546.503 kb on + strand at 546.521 kb on + strand at 546.521 kb on + strand at 546.589 kb on - strand at 546.590 kb on + strand at 546.690 kb on - strand, within MMP_RS02870 at 546.690 kb on - strand, within MMP_RS02870 at 546.690 kb on - strand, within MMP_RS02870 at 546.697 kb on + strand, within MMP_RS02870 at 546.784 kb on - strand, within MMP_RS02870 at 546.792 kb on + strand, within MMP_RS02870 at 546.865 kb on - strand, within MMP_RS02870 at 547.066 kb on + strand, within MMP_RS02870 at 547.074 kb on - strand, within MMP_RS02870 at 547.075 kb on + strand, within MMP_RS02870 at 547.095 kb on - strand at 547.107 kb on - strand at 547.123 kb on + strand at 547.123 kb on + strand at 547.161 kb on + strand at 547.173 kb on - strand at 547.183 kb on - strand at 547.192 kb on - strand at 547.236 kb on - strand, within MMP_RS02875 at 547.285 kb on - strand, within MMP_RS02875 at 547.289 kb on + strand, within MMP_RS02875 at 547.299 kb on - strand, within MMP_RS02875 at 547.341 kb on + strand, within MMP_RS02875 at 547.363 kb on + strand, within MMP_RS02875 at 547.367 kb on - strand, within MMP_RS02875
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 544,923 + -3.7 544,945 + +0.9 544,951 - -2.5 544,952 + -0.1 544,952 + -0.3 544,964 - -4.1 544,965 + -2.4 545,248 + -1.7 545,430 + -1.0 545,612 + -2.3 546,064 + -4.6 546,306 + -4.5 546,498 + -0.2 546,503 + -0.7 546,521 + -1.3 546,521 + -0.8 546,589 - +0.4 546,590 + -2.1 546,690 - MMP_RS02870 0.24 -0.8 546,690 - MMP_RS02870 0.24 -0.3 546,690 - MMP_RS02870 0.24 -1.5 546,697 + MMP_RS02870 0.25 -0.3 546,784 - MMP_RS02870 0.39 -0.4 546,792 + MMP_RS02870 0.40 -1.7 546,865 - MMP_RS02870 0.53 -0.8 547,066 + MMP_RS02870 0.86 -0.9 547,074 - MMP_RS02870 0.87 +0.4 547,075 + MMP_RS02870 0.87 -0.6 547,095 - -0.5 547,107 - -0.2 547,123 + -0.9 547,123 + -0.3 547,161 + +0.9 547,173 - +0.3 547,183 - -0.9 547,192 - -1.4 547,236 - MMP_RS02875 0.10 -1.5 547,285 - MMP_RS02875 0.20 -2.0 547,289 + MMP_RS02875 0.20 -0.3 547,299 - MMP_RS02875 0.22 +0.0 547,341 + MMP_RS02875 0.30 -1.3 547,363 + MMP_RS02875 0.35 -0.9 547,367 - MMP_RS02875 0.35 -0.4
Or see this region's nucleotide sequence