Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02855 and MMP_RS02860 are separated by 98 nucleotides MMP_RS02860 and MMP_RS02865 are separated by 36 nucleotides MMP_RS02865 and MMP_RS02870 are separated by 125 nucleotides
MMP_RS02855: MMP_RS02855 - 3-isopropylmalate dehydrogenase, at 543,913 to 544,905
_RS02855
MMP_RS02860: MMP_RS02860 - phosphoribosylaminoimidazolesuccinocarboxamide synthase, at 545,004 to 545,744
_RS02860
MMP_RS02865: MMP_RS02865 - phosphoserine phosphatase SerB, at 545,781 to 546,422
_RS02865
MMP_RS02870: MMP_RS02870 - hypothetical protein, at 546,548 to 547,150
_RS02870
Position (kb) 545
546 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 544.055 kb on - strand at 544.181 kb on - strand at 544.198 kb on - strand at 544.235 kb on + strand at 544.239 kb on + strand at 544.241 kb on - strand at 544.494 kb on - strand at 544.513 kb on - strand at 544.772 kb on - strand at 544.923 kb on + strand at 544.945 kb on + strand at 544.951 kb on - strand at 544.952 kb on + strand at 544.952 kb on + strand at 544.964 kb on - strand at 544.965 kb on + strand at 545.248 kb on + strand at 545.430 kb on + strand at 545.612 kb on + strand at 546.064 kb on + strand at 546.306 kb on + strand at 546.498 kb on + strand at 546.503 kb on + strand at 546.521 kb on + strand at 546.521 kb on + strand at 546.589 kb on - strand at 546.590 kb on + strand at 546.690 kb on - strand, within MMP_RS02870 at 546.690 kb on - strand, within MMP_RS02870 at 546.690 kb on - strand, within MMP_RS02870 at 546.697 kb on + strand, within MMP_RS02870
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 544,055 - -2.4 544,181 - -4.0 544,198 - -3.1 544,235 + -4.6 544,239 + -4.2 544,241 - -3.4 544,494 - -0.9 544,513 - -2.2 544,772 - -1.3 544,923 + -3.7 544,945 + +0.9 544,951 - -2.5 544,952 + -0.1 544,952 + -0.3 544,964 - -4.1 544,965 + -2.4 545,248 + -1.7 545,430 + -1.0 545,612 + -2.3 546,064 + -4.6 546,306 + -4.5 546,498 + -0.2 546,503 + -0.7 546,521 + -1.3 546,521 + -0.8 546,589 - +0.4 546,590 + -2.1 546,690 - MMP_RS02870 0.24 -0.8 546,690 - MMP_RS02870 0.24 -0.3 546,690 - MMP_RS02870 0.24 -1.5 546,697 + MMP_RS02870 0.25 -0.3
Or see this region's nucleotide sequence