Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02260 and MMP_RS02265 are separated by 13 nucleotides MMP_RS02265 and MMP_RS02270 are separated by 62 nucleotides MMP_RS02270 and MMP_RS02275 are separated by 89 nucleotides
MMP_RS02260: MMP_RS02260 - lactate utilization protein, at 426,937 to 427,578
_RS02260
MMP_RS02265: MMP_RS02265 - nitroreductase family protein, at 427,592 to 428,161
_RS02265
MMP_RS02270: MMP_RS02270 - replication factor C small subunit, at 428,224 to 429,171
_RS02270
MMP_RS02275: MMP_RS02275 - Ni-sirohydrochlorin a c-diamide reductive cyclase catalytic subunit, at 429,261 to 430,361
_RS02275
Position (kb) 428
429
430 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 427.232 kb on - strand, within MMP_RS02260 at 427.236 kb on - strand, within MMP_RS02260 at 427.277 kb on - strand, within MMP_RS02260 at 427.277 kb on - strand, within MMP_RS02260 at 427.356 kb on - strand, within MMP_RS02260 at 427.469 kb on - strand, within MMP_RS02260 at 427.505 kb on + strand, within MMP_RS02260 at 427.516 kb on + strand at 427.539 kb on - strand at 427.540 kb on + strand at 427.557 kb on + strand at 427.735 kb on - strand, within MMP_RS02265 at 428.018 kb on - strand, within MMP_RS02265 at 428.019 kb on + strand, within MMP_RS02265 at 428.024 kb on + strand, within MMP_RS02265 at 428.151 kb on + strand at 428.225 kb on - strand at 428.230 kb on - strand at 428.261 kb on + strand at 428.384 kb on + strand at 428.387 kb on + strand at 428.427 kb on + strand at 428.606 kb on - strand at 429.090 kb on - strand at 429.193 kb on - strand at 429.209 kb on - strand at 429.234 kb on - strand at 429.235 kb on + strand at 429.242 kb on - strand at 429.273 kb on + strand at 429.385 kb on - strand at 429.386 kb on + strand at 429.829 kb on - strand at 430.009 kb on + strand at 430.018 kb on + strand at 430.021 kb on + strand at 430.155 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 427,232 - MMP_RS02260 0.46 -0.1 427,236 - MMP_RS02260 0.47 -0.2 427,277 - MMP_RS02260 0.53 +0.9 427,277 - MMP_RS02260 0.53 -0.8 427,356 - MMP_RS02260 0.65 +1.0 427,469 - MMP_RS02260 0.83 +0.4 427,505 + MMP_RS02260 0.88 -0.8 427,516 + +0.1 427,539 - -0.2 427,540 + +0.3 427,557 + +0.2 427,735 - MMP_RS02265 0.25 -2.0 428,018 - MMP_RS02265 0.75 +0.6 428,019 + MMP_RS02265 0.75 +0.1 428,024 + MMP_RS02265 0.76 +0.6 428,151 + +0.5 428,225 - +0.6 428,230 - +0.0 428,261 + -3.0 428,384 + -1.2 428,387 + -1.2 428,427 + -0.2 428,606 - -3.3 429,090 - -1.2 429,193 - -1.8 429,209 - -1.0 429,234 - -0.8 429,235 + +0.1 429,242 - -0.0 429,273 + +0.5 429,385 - -2.4 429,386 + -0.2 429,829 - +0.2 430,009 + -1.0 430,018 + -2.2 430,021 + -2.0 430,155 - -0.4
Or see this region's nucleotide sequence