Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02155 and MMP_RS02160 are separated by 105 nucleotides MMP_RS02160 and MMP_RS02165 are separated by 27 nucleotides MMP_RS02165 and MMP_RS02170 are separated by 14 nucleotides
MMP_RS02155: MMP_RS02155 - 2-phospho-L-lactate transferase, at 403,481 to 404,410
_RS02155
MMP_RS02160: MMP_RS02160 - GTP cyclohydrolase IIa, at 404,516 to 405,316
_RS02160
MMP_RS02165: MMP_RS02165 - tRNA-dihydrouridine synthase, at 405,344 to 406,114
_RS02165
MMP_RS02170: MMP_RS02170 - CBS domain-containing protein, at 406,129 to 406,542
_RS02170
Position (kb) 404
405
406 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 403.592 kb on + strand at 403.595 kb on - strand at 403.595 kb on - strand at 403.595 kb on - strand at 403.595 kb on - strand at 403.596 kb on + strand at 403.597 kb on - strand at 403.611 kb on + strand at 403.611 kb on + strand at 403.811 kb on + strand at 403.811 kb on + strand at 403.811 kb on + strand at 403.984 kb on - strand at 403.986 kb on - strand at 403.987 kb on + strand at 404.163 kb on - strand at 404.164 kb on + strand at 404.164 kb on + strand at 404.244 kb on + strand at 404.250 kb on + strand at 404.260 kb on - strand at 404.265 kb on - strand at 404.266 kb on + strand at 404.287 kb on - strand at 404.293 kb on - strand at 404.293 kb on - strand at 404.294 kb on + strand at 404.294 kb on + strand at 404.329 kb on + strand at 404.452 kb on - strand at 404.455 kb on + strand at 404.500 kb on + strand at 404.511 kb on - strand at 404.619 kb on + strand at 405.112 kb on - strand at 405.289 kb on + strand at 405.294 kb on + strand at 405.299 kb on - strand at 405.299 kb on - strand at 405.354 kb on - strand at 405.434 kb on - strand, within MMP_RS02165 at 405.657 kb on - strand, within MMP_RS02165 at 405.658 kb on + strand, within MMP_RS02165 at 405.682 kb on - strand, within MMP_RS02165 at 405.842 kb on + strand, within MMP_RS02165 at 405.910 kb on - strand, within MMP_RS02165 at 405.910 kb on - strand, within MMP_RS02165 at 405.910 kb on - strand, within MMP_RS02165 at 405.911 kb on + strand, within MMP_RS02165 at 405.913 kb on + strand, within MMP_RS02165 at 405.913 kb on + strand, within MMP_RS02165 at 405.913 kb on + strand, within MMP_RS02165 at 405.913 kb on + strand, within MMP_RS02165 at 405.913 kb on + strand, within MMP_RS02165 at 405.913 kb on + strand, within MMP_RS02165 at 405.949 kb on + strand, within MMP_RS02165 at 406.078 kb on + strand at 406.087 kb on - strand at 406.087 kb on - strand at 406.088 kb on + strand at 406.117 kb on + strand at 406.117 kb on + strand at 406.117 kb on + strand at 406.121 kb on - strand at 406.130 kb on - strand at 406.135 kb on - strand at 406.200 kb on - strand, within MMP_RS02170 at 406.220 kb on + strand, within MMP_RS02170
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 403,592 + -2.9 403,595 - -1.6 403,595 - -2.9 403,595 - -3.9 403,595 - -4.3 403,596 + -2.6 403,597 - -3.0 403,611 + -3.4 403,611 + -0.6 403,811 + -2.1 403,811 + -1.5 403,811 + -3.5 403,984 - -1.7 403,986 - -1.7 403,987 + -5.3 404,163 - -4.9 404,164 + -2.9 404,164 + -1.8 404,244 + -2.6 404,250 + -1.1 404,260 - -5.7 404,265 - -1.5 404,266 + -2.6 404,287 - -2.1 404,293 - -2.2 404,293 - -2.4 404,294 + -2.8 404,294 + -3.2 404,329 + -3.1 404,452 - -1.9 404,455 + -3.5 404,500 + -4.8 404,511 - -3.2 404,619 + -3.7 405,112 - -2.1 405,289 + -3.2 405,294 + +0.4 405,299 - -1.3 405,299 - +0.0 405,354 - -3.1 405,434 - MMP_RS02165 0.12 -0.4 405,657 - MMP_RS02165 0.41 -1.1 405,658 + MMP_RS02165 0.41 -2.2 405,682 - MMP_RS02165 0.44 +0.4 405,842 + MMP_RS02165 0.65 -0.0 405,910 - MMP_RS02165 0.73 -1.1 405,910 - MMP_RS02165 0.73 -0.5 405,910 - MMP_RS02165 0.73 -1.7 405,911 + MMP_RS02165 0.74 -2.9 405,913 + MMP_RS02165 0.74 -2.2 405,913 + MMP_RS02165 0.74 -0.3 405,913 + MMP_RS02165 0.74 -0.8 405,913 + MMP_RS02165 0.74 -1.5 405,913 + MMP_RS02165 0.74 -1.1 405,913 + MMP_RS02165 0.74 -0.7 405,949 + MMP_RS02165 0.78 -0.7 406,078 + -0.5 406,087 - +0.3 406,087 - +0.2 406,088 + -0.2 406,117 + -0.2 406,117 + -0.5 406,117 + +0.8 406,121 - +1.0 406,130 - -0.0 406,135 - +0.4 406,200 - MMP_RS02170 0.17 +0.1 406,220 + MMP_RS02170 0.22 +0.2
Or see this region's nucleotide sequence