Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02115 and MMP_RS02120 are separated by 201 nucleotides MMP_RS02120 and MMP_RS02125 are separated by 41 nucleotides MMP_RS02125 and MMP_RS02130 are separated by 10 nucleotides
MMP_RS02115: MMP_RS02115 - phosphopyruvate hydratase, at 393,105 to 394,397
_RS02115
MMP_RS02120: MMP_RS02120 - alanine--tRNA ligase, at 394,599 to 397,277
_RS02120
MMP_RS02125: MMP_RS02125 - pantoate kinase, at 397,319 to 398,191
_RS02125
MMP_RS02130: MMP_RS02130 - zinc metalloprotease HtpX, at 398,202 to 399,059
_RS02130
Position (kb)
394
395
396
397
398 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 393.926 kb on + strand at 393.937 kb on + strand at 394.320 kb on - strand at 394.401 kb on - strand at 394.406 kb on + strand at 394.443 kb on - strand at 394.453 kb on + strand at 394.479 kb on + strand at 394.491 kb on - strand at 394.577 kb on + strand at 394.620 kb on + strand at 394.779 kb on - strand at 394.780 kb on + strand at 395.033 kb on - strand at 395.669 kb on - strand at 395.788 kb on + strand at 395.790 kb on + strand at 395.914 kb on + strand at 397.131 kb on + strand at 397.309 kb on + strand at 397.336 kb on - strand at 397.361 kb on + strand at 397.870 kb on - strand at 397.871 kb on + strand at 397.872 kb on - strand at 397.873 kb on + strand at 397.900 kb on + strand at 397.998 kb on - strand at 397.998 kb on - strand at 398.136 kb on - strand at 398.190 kb on + strand at 398.211 kb on + strand at 398.268 kb on - strand at 398.269 kb on + strand at 398.271 kb on + strand at 398.271 kb on + strand at 398.271 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 393,926 + -4.2 393,937 + -3.3 394,320 - -1.4 394,401 - +1.1 394,406 + +0.3 394,443 - +0.0 394,453 + +0.8 394,479 + -1.8 394,491 - -0.6 394,577 + -3.0 394,620 + -2.7 394,779 - -2.3 394,780 + -1.0 395,033 - -2.5 395,669 - -3.2 395,788 + -3.1 395,790 + +0.8 395,914 + -1.7 397,131 + -3.3 397,309 + -1.8 397,336 - -0.3 397,361 + -0.9 397,870 - -2.4 397,871 + -1.5 397,872 - -2.8 397,873 + -3.2 397,900 + -2.2 397,998 - -2.3 397,998 - -2.6 398,136 - -2.5 398,190 + -1.3 398,211 + -0.5 398,268 - +0.2 398,269 + -1.0 398,271 + +0.4 398,271 + +0.1 398,271 + -0.3
Or see this region's nucleotide sequence