Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02060 and MMP_RS02065 are separated by 236 nucleotides MMP_RS02065 and MMP_RS02070 are separated by 289 nucleotides MMP_RS02070 and MMP_RS02075 are separated by 31 nucleotides
MMP_RS02060: MMP_RS02060 - UMP kinase, at 384,368 to 385,045
_RS02060
MMP_RS02065: MMP_RS02065 - histone family protein, at 385,282 to 385,485
_RS02065
MMP_RS02070: MMP_RS02070 - hypothetical protein, at 385,775 to 386,296
_RS02070
MMP_RS02075: MMP_RS02075 - NAD(P)/FAD-dependent oxidoreductase, at 386,328 to 387,500
_RS02075
Position (kb)
385
386 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 384.306 kb on - strand at 384.307 kb on + strand at 384.310 kb on - strand at 384.354 kb on + strand at 384.740 kb on + strand at 385.122 kb on - strand at 385.180 kb on - strand at 385.180 kb on - strand at 385.193 kb on - strand at 385.193 kb on - strand at 385.231 kb on - strand at 385.292 kb on + strand at 385.571 kb on + strand at 385.609 kb on - strand at 385.614 kb on - strand at 385.619 kb on - strand at 385.624 kb on - strand at 385.628 kb on - strand at 385.629 kb on + strand at 385.633 kb on - strand at 385.634 kb on + strand at 385.668 kb on + strand at 386.072 kb on - strand at 386.122 kb on + strand at 386.176 kb on + strand at 386.244 kb on - strand at 386.247 kb on - strand at 386.255 kb on - strand at 386.259 kb on + strand at 386.259 kb on + strand at 386.301 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 384,306 - -0.4 384,307 + -1.2 384,310 - -0.2 384,354 + -0.2 384,740 + +0.8 385,122 - -1.0 385,180 - -0.3 385,180 - +0.1 385,193 - -0.1 385,193 - -0.1 385,231 - +0.7 385,292 + -1.9 385,571 + -0.2 385,609 - -0.7 385,614 - -0.5 385,619 - +0.0 385,624 - -0.2 385,628 - +0.2 385,629 + +0.2 385,633 - +1.9 385,634 + -0.1 385,668 + +0.5 386,072 - -2.5 386,122 + -0.2 386,176 + -0.2 386,244 - -0.1 386,247 - +1.4 386,255 - +0.9 386,259 + +1.1 386,259 + -0.4 386,301 - +0.4
Or see this region's nucleotide sequence