Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02025 and MMP_RS02030 are separated by 179 nucleotides MMP_RS02030 and MMP_RS02035 are separated by 78 nucleotides MMP_RS02035 and MMP_RS02040 are separated by 417 nucleotides
MMP_RS02025: MMP_RS02025 - DNA-directed DNA polymerase, at 376,490 to 378,844
_RS02025
MMP_RS02030: MMP_RS02030 - homoaconitase small subunit, at 379,024 to 379,512
_RS02030
MMP_RS02035: MMP_RS02035 - ribosome biogenesis/translation initiation ATPase RLI, at 379,591 to 381,363
_RS02035
MMP_RS02040: MMP_RS02040 - S-layer protein, at 381,781 to 383,508
_RS02040
Position (kb)
379
380
381
382 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 378.770 kb on - strand at 378.771 kb on + strand at 378.771 kb on + strand at 378.782 kb on - strand at 378.791 kb on - strand at 378.791 kb on - strand at 378.791 kb on - strand at 378.792 kb on + strand at 378.792 kb on + strand at 378.792 kb on + strand at 378.795 kb on - strand at 378.796 kb on + strand at 378.796 kb on + strand at 378.925 kb on - strand at 378.964 kb on - strand at 379.190 kb on - strand at 379.380 kb on + strand at 379.550 kb on + strand at 379.557 kb on + strand at 379.615 kb on - strand at 379.864 kb on - strand at 380.444 kb on + strand at 380.472 kb on + strand at 380.922 kb on - strand at 381.071 kb on - strand at 381.159 kb on - strand at 381.402 kb on + strand at 381.413 kb on + strand at 381.433 kb on + strand at 381.441 kb on + strand at 381.448 kb on - strand at 381.759 kb on + strand at 381.803 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 378,770 - -0.5 378,771 + +0.3 378,771 + +0.7 378,782 - +0.5 378,791 - +0.2 378,791 - -0.1 378,791 - +1.0 378,792 + +0.5 378,792 + +0.6 378,792 + +0.5 378,795 - -0.4 378,796 + +0.4 378,796 + -0.3 378,925 - -0.9 378,964 - -3.9 379,190 - -3.3 379,380 + -0.7 379,550 + -0.1 379,557 + +0.5 379,615 - -3.2 379,864 - -3.0 380,444 + -3.7 380,472 + -1.4 380,922 - -3.6 381,071 - -3.0 381,159 - -2.9 381,402 + -0.5 381,413 + +0.6 381,433 + -1.9 381,441 + -0.7 381,448 - +0.9 381,759 + -3.6 381,803 - -4.5
Or see this region's nucleotide sequence