Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02025 and MMP_RS02030 are separated by 179 nucleotides MMP_RS02030 and MMP_RS02035 are separated by 78 nucleotides
MMP_RS02025: MMP_RS02025 - DNA-directed DNA polymerase, at 376,490 to 378,844
_RS02025
MMP_RS02030: MMP_RS02030 - homoaconitase small subunit, at 379,024 to 379,512
_RS02030
MMP_RS02035: MMP_RS02035 - ribosome biogenesis/translation initiation ATPase RLI, at 379,591 to 381,363
_RS02035
Position (kb)
379
380 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 378.064 kb on + strand, within MMP_RS02025 at 378.261 kb on - strand, within MMP_RS02025 at 378.418 kb on - strand, within MMP_RS02025 at 378.473 kb on + strand, within MMP_RS02025 at 378.506 kb on + strand, within MMP_RS02025 at 378.540 kb on + strand, within MMP_RS02025 at 378.770 kb on - strand at 378.771 kb on + strand at 378.771 kb on + strand at 378.782 kb on - strand at 378.791 kb on - strand at 378.791 kb on - strand at 378.791 kb on - strand at 378.792 kb on + strand at 378.792 kb on + strand at 378.792 kb on + strand at 378.795 kb on - strand at 378.796 kb on + strand at 378.796 kb on + strand at 378.925 kb on - strand at 378.964 kb on - strand at 379.190 kb on - strand at 379.380 kb on + strand at 379.550 kb on + strand at 379.557 kb on + strand at 379.615 kb on - strand at 379.864 kb on - strand at 380.444 kb on + strand at 380.472 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 378,064 + MMP_RS02025 0.67 -0.1 378,261 - MMP_RS02025 0.75 +0.4 378,418 - MMP_RS02025 0.82 -0.3 378,473 + MMP_RS02025 0.84 -0.0 378,506 + MMP_RS02025 0.86 +0.5 378,540 + MMP_RS02025 0.87 -1.1 378,770 - -0.5 378,771 + +0.3 378,771 + +0.7 378,782 - +0.5 378,791 - +0.2 378,791 - -0.1 378,791 - +1.0 378,792 + +0.5 378,792 + +0.6 378,792 + +0.5 378,795 - -0.4 378,796 + +0.4 378,796 + -0.3 378,925 - -0.9 378,964 - -3.9 379,190 - -3.3 379,380 + -0.7 379,550 + -0.1 379,557 + +0.5 379,615 - -3.2 379,864 - -3.0 380,444 + -3.7 380,472 + -1.4
Or see this region's nucleotide sequence