Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01805 and MMP_RS01810 are separated by 110 nucleotides MMP_RS01810 and MMP_RS01815 are separated by 27 nucleotides MMP_RS01815 and MMP_RS01820 are separated by 212 nucleotides MMP_RS01820 and MMP_RS01825 are separated by 45 nucleotides
MMP_RS01805: MMP_RS01805 - NUDIX hydrolase, at 334,861 to 335,376
_RS01805
MMP_RS01810: MMP_RS01810 - tRNA-Arg, at 335,487 to 335,564
_RS01810
MMP_RS01815: MMP_RS01815 - tRNA-Glu, at 335,592 to 335,666
_RS01815
MMP_RS01820: MMP_RS01820 - sodium-extruding oxaloacetate decarboxylase subunit alpha, at 335,879 to 337,588
_RS01820
MMP_RS01825: MMP_RS01825 - acetyl-CoA carboxylase biotin carboxylase subunit, at 337,634 to 339,139
_RS01825
Position (kb) 335
336
337
338 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 335.014 kb on - strand, within MMP_RS01805 at 335.056 kb on - strand, within MMP_RS01805 at 335.292 kb on - strand, within MMP_RS01805 at 335.293 kb on + strand, within MMP_RS01805 at 335.306 kb on - strand, within MMP_RS01805 at 335.362 kb on - strand at 335.363 kb on + strand at 335.364 kb on - strand at 335.364 kb on - strand at 335.364 kb on - strand at 335.366 kb on - strand at 335.398 kb on + strand at 335.458 kb on - strand at 335.721 kb on - strand at 335.775 kb on + strand at 335.790 kb on + strand at 336.034 kb on - strand at 336.516 kb on + strand at 336.517 kb on - strand at 336.517 kb on - strand at 336.518 kb on + strand at 336.787 kb on + strand at 336.974 kb on + strand at 337.064 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 335,014 - MMP_RS01805 0.30 +0.2 335,056 - MMP_RS01805 0.38 +0.2 335,292 - MMP_RS01805 0.84 -2.8 335,293 + MMP_RS01805 0.84 +0.7 335,306 - MMP_RS01805 0.86 +0.4 335,362 - -0.3 335,363 + +0.0 335,364 - +0.9 335,364 - +0.0 335,364 - +0.4 335,366 - -0.1 335,398 + +0.3 335,458 - -1.1 335,721 - +0.6 335,775 + -3.2 335,790 + -2.7 336,034 - -3.5 336,516 + -1.7 336,517 - -3.1 336,517 - -3.0 336,518 + -2.5 336,787 + -3.1 336,974 + -3.2 337,064 + -2.5
Or see this region's nucleotide sequence