Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01750 and MMP_RS01755 are separated by 14 nucleotides MMP_RS01755 and MMP_RS01760 are separated by 14 nucleotides MMP_RS01760 and MMP_RS01765 are separated by 1 nucleotides MMP_RS01765 and MMP_RS01770 are separated by 154 nucleotides
MMP_RS01750: MMP_RS01750 - DUF63 family protein, at 326,152 to 326,958
_RS01750
MMP_RS01755: MMP_RS01755 - DUF2097 domain-containing protein, at 326,973 to 327,263
_RS01755
MMP_RS01760: MMP_RS01760 - cobalt-precorrin-8 methylmutase, at 327,278 to 327,910
_RS01760
MMP_RS01765: MMP_RS01765 - HIT family protein, at 327,912 to 328,304
_RS01765
MMP_RS01770: MMP_RS01770 - ATP-dependent endonuclease, at 328,459 to 330,339
_RS01770
Position (kb) 327
328 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 326.338 kb on + strand, within MMP_RS01750 at 326.445 kb on - strand, within MMP_RS01750 at 326.445 kb on - strand, within MMP_RS01750 at 326.449 kb on - strand, within MMP_RS01750 at 326.450 kb on + strand, within MMP_RS01750 at 326.501 kb on + strand, within MMP_RS01750 at 326.501 kb on + strand, within MMP_RS01750 at 326.505 kb on + strand, within MMP_RS01750 at 326.585 kb on - strand, within MMP_RS01750 at 326.590 kb on - strand, within MMP_RS01750 at 326.590 kb on - strand, within MMP_RS01750 at 326.590 kb on - strand, within MMP_RS01750 at 326.590 kb on - strand, within MMP_RS01750 at 326.590 kb on - strand, within MMP_RS01750 at 326.591 kb on + strand, within MMP_RS01750 at 326.591 kb on + strand, within MMP_RS01750 at 326.591 kb on + strand, within MMP_RS01750 at 326.603 kb on - strand, within MMP_RS01750 at 326.613 kb on + strand, within MMP_RS01750 at 326.617 kb on - strand, within MMP_RS01750 at 326.620 kb on - strand, within MMP_RS01750 at 326.620 kb on - strand, within MMP_RS01750 at 326.621 kb on + strand, within MMP_RS01750 at 326.633 kb on + strand, within MMP_RS01750 at 326.837 kb on - strand, within MMP_RS01750 at 326.881 kb on + strand at 327.036 kb on - strand, within MMP_RS01755 at 327.052 kb on - strand, within MMP_RS01755 at 327.268 kb on - strand at 328.188 kb on - strand, within MMP_RS01765 at 328.189 kb on + strand, within MMP_RS01765 at 328.191 kb on - strand, within MMP_RS01765 at 328.196 kb on - strand, within MMP_RS01765 at 328.197 kb on + strand, within MMP_RS01765 at 328.226 kb on - strand, within MMP_RS01765 at 328.257 kb on - strand, within MMP_RS01765 at 328.308 kb on - strand at 328.318 kb on - strand at 328.319 kb on + strand at 328.319 kb on + strand at 328.319 kb on + strand at 328.354 kb on - strand at 328.354 kb on - strand at 328.354 kb on - strand at 328.355 kb on + strand at 328.356 kb on - strand at 328.358 kb on - strand at 328.359 kb on + strand at 328.359 kb on + strand at 328.416 kb on - strand at 328.475 kb on - strand at 328.479 kb on - strand at 328.479 kb on - strand at 328.563 kb on - strand at 328.576 kb on + strand at 328.577 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 326,338 + MMP_RS01750 0.23 -0.5 326,445 - MMP_RS01750 0.36 -0.5 326,445 - MMP_RS01750 0.36 -0.3 326,449 - MMP_RS01750 0.37 -2.7 326,450 + MMP_RS01750 0.37 -1.6 326,501 + MMP_RS01750 0.43 -0.9 326,501 + MMP_RS01750 0.43 -0.4 326,505 + MMP_RS01750 0.44 -0.2 326,585 - MMP_RS01750 0.54 +1.8 326,590 - MMP_RS01750 0.54 -1.2 326,590 - MMP_RS01750 0.54 -0.4 326,590 - MMP_RS01750 0.54 +0.7 326,590 - MMP_RS01750 0.54 -0.9 326,590 - MMP_RS01750 0.54 -0.7 326,591 + MMP_RS01750 0.54 +0.8 326,591 + MMP_RS01750 0.54 -0.1 326,591 + MMP_RS01750 0.54 +0.7 326,603 - MMP_RS01750 0.56 -0.5 326,613 + MMP_RS01750 0.57 -0.2 326,617 - MMP_RS01750 0.58 -2.6 326,620 - MMP_RS01750 0.58 +0.0 326,620 - MMP_RS01750 0.58 -0.7 326,621 + MMP_RS01750 0.58 +0.4 326,633 + MMP_RS01750 0.60 +0.3 326,837 - MMP_RS01750 0.85 -2.0 326,881 + -0.5 327,036 - MMP_RS01755 0.22 -1.1 327,052 - MMP_RS01755 0.27 -5.3 327,268 - -3.5 328,188 - MMP_RS01765 0.70 +0.7 328,189 + MMP_RS01765 0.70 -1.3 328,191 - MMP_RS01765 0.71 +0.1 328,196 - MMP_RS01765 0.72 -0.3 328,197 + MMP_RS01765 0.73 -0.3 328,226 - MMP_RS01765 0.80 +0.0 328,257 - MMP_RS01765 0.88 +0.4 328,308 - -0.3 328,318 - -0.5 328,319 + -0.1 328,319 + +0.8 328,319 + +0.5 328,354 - -0.2 328,354 - +0.2 328,354 - -0.5 328,355 + -0.0 328,356 - +0.9 328,358 - +0.0 328,359 + -0.0 328,359 + +0.5 328,416 - -0.2 328,475 - +0.6 328,479 - +1.2 328,479 - +0.4 328,563 - +0.7 328,576 + -1.0 328,577 - -0.2
Or see this region's nucleotide sequence