Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01735 and MMP_RS01740 are separated by 206 nucleotides MMP_RS01740 and MMP_RS01745 are separated by 135 nucleotides
MMP_RS01735: MMP_RS01735 - type II glyceraldehyde-3-phosphate dehydrogenase, at 321,169 to 322,191
_RS01735
MMP_RS01740: MMP_RS01740 - methionine--tRNA ligase, at 322,398 to 324,389
_RS01740
MMP_RS01745: MMP_RS01745 - AMP phosphorylase, at 324,525 to 326,042
_RS01745
Position (kb)
322
323
324
325 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 321.951 kb on - strand at 321.952 kb on + strand at 322.226 kb on - strand at 322.258 kb on - strand at 322.262 kb on - strand at 322.265 kb on + strand at 322.271 kb on - strand at 322.272 kb on + strand at 322.275 kb on - strand at 322.276 kb on + strand at 322.277 kb on - strand at 322.280 kb on - strand at 322.285 kb on - strand at 322.286 kb on + strand at 322.287 kb on - strand at 322.288 kb on + strand at 322.327 kb on + strand at 322.328 kb on - strand at 322.409 kb on + strand at 322.413 kb on + strand at 322.428 kb on - strand at 322.920 kb on + strand at 323.802 kb on - strand at 323.842 kb on - strand at 323.945 kb on + strand at 323.945 kb on + strand at 323.948 kb on - strand at 323.970 kb on + strand at 324.212 kb on + strand at 324.220 kb on + strand at 324.332 kb on - strand at 324.337 kb on - strand at 324.337 kb on - strand at 324.390 kb on - strand at 324.406 kb on - strand at 324.459 kb on - strand at 324.468 kb on - strand at 324.485 kb on - strand at 324.550 kb on - strand at 324.637 kb on - strand at 324.767 kb on + strand, within MMP_RS01745 at 324.918 kb on - strand, within MMP_RS01745 at 324.920 kb on - strand, within MMP_RS01745 at 325.025 kb on - strand, within MMP_RS01745 at 325.072 kb on - strand, within MMP_RS01745
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 321,951 - -2.9 321,952 + -1.5 322,226 - -1.9 322,258 - +1.4 322,262 - -1.0 322,265 + -0.9 322,271 - +1.1 322,272 + -0.3 322,275 - +1.4 322,276 + +0.3 322,277 - +0.2 322,280 - +1.1 322,285 - +0.8 322,286 + -0.1 322,287 - +0.6 322,288 + +0.7 322,327 + -1.4 322,328 - -3.1 322,409 + -0.3 322,413 + -1.3 322,428 - -3.9 322,920 + -1.8 323,802 - -1.9 323,842 - -1.1 323,945 + -1.7 323,945 + -2.5 323,948 - -2.1 323,970 + -2.3 324,212 + -3.6 324,220 + +0.1 324,332 - -3.0 324,337 - -0.5 324,337 - +0.3 324,390 - -0.7 324,406 - -0.2 324,459 - +0.3 324,468 - +1.3 324,485 - +0.8 324,550 - +0.8 324,637 - +0.1 324,767 + MMP_RS01745 0.16 +0.5 324,918 - MMP_RS01745 0.26 +1.1 324,920 - MMP_RS01745 0.26 +0.1 325,025 - MMP_RS01745 0.33 +1.0 325,072 - MMP_RS01745 0.36 -1.3
Or see this region's nucleotide sequence