Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01585 and MMP_RS01590 are separated by 37 nucleotides MMP_RS01590 and MMP_RS01595 are separated by 57 nucleotides MMP_RS01595 and MMP_RS01600 are separated by 226 nucleotides
MMP_RS01585: MMP_RS01585 - homoserine kinase, at 295,253 to 296,158
_RS01585
MMP_RS01590: MMP_RS01590 - hypothetical protein, at 296,196 to 297,284
_RS01590
MMP_RS01595: MMP_RS01595 - translation initiation factor IF-2 subunit beta, at 297,342 to 297,758
_RS01595
MMP_RS01600: MMP_RS01600 - 50S ribosomal protein L15e, at 297,985 to 298,569
_RS01600
Position (kb) 296
297
298 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 295.270 kb on + strand at 295.447 kb on + strand at 296.009 kb on + strand at 296.009 kb on + strand at 296.064 kb on - strand at 296.193 kb on - strand at 296.198 kb on + strand at 296.202 kb on - strand at 296.285 kb on - strand at 296.455 kb on - strand at 296.564 kb on - strand at 296.845 kb on - strand at 296.855 kb on - strand at 296.856 kb on + strand at 296.927 kb on - strand at 297.045 kb on + strand at 297.315 kb on - strand at 297.319 kb on - strand at 297.843 kb on + strand at 297.874 kb on - strand at 297.891 kb on + strand at 298.001 kb on + strand at 298.141 kb on - strand, within MMP_RS01600 at 298.183 kb on + strand, within MMP_RS01600
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 295,270 + -4.4 295,447 + -3.2 296,009 + -2.0 296,009 + -2.4 296,064 - -3.1 296,193 - +0.1 296,198 + -1.1 296,202 - -0.1 296,285 - -0.6 296,455 - -0.6 296,564 - -3.0 296,845 - -0.9 296,855 - -0.7 296,856 + -2.8 296,927 - -4.2 297,045 + +0.2 297,315 - -0.4 297,319 - +0.1 297,843 + -2.6 297,874 - -3.6 297,891 + -2.9 298,001 + -2.1 298,141 - MMP_RS01600 0.27 -3.9 298,183 + MMP_RS01600 0.34 -2.6
Or see this region's nucleotide sequence