Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01580 and MMP_RS01585 are separated by 123 nucleotides MMP_RS01585 and MMP_RS01590 are separated by 37 nucleotides
MMP_RS01580: MMP_RS01580 - PQQ-binding-like beta-propeller repeat protein, at 294,281 to 295,129
_RS01580
MMP_RS01585: MMP_RS01585 - homoserine kinase, at 295,253 to 296,158
_RS01585
MMP_RS01590: MMP_RS01590 - hypothetical protein, at 296,196 to 297,284
_RS01590
Position (kb) 295
296
297 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 294.338 kb on + strand at 294.338 kb on + strand at 294.377 kb on + strand, within MMP_RS01580 at 294.377 kb on + strand, within MMP_RS01580 at 294.398 kb on + strand, within MMP_RS01580 at 294.398 kb on + strand, within MMP_RS01580 at 294.459 kb on - strand, within MMP_RS01580 at 294.470 kb on + strand, within MMP_RS01580 at 294.515 kb on + strand, within MMP_RS01580 at 294.637 kb on + strand, within MMP_RS01580 at 294.752 kb on - strand, within MMP_RS01580 at 294.753 kb on + strand, within MMP_RS01580 at 294.870 kb on - strand, within MMP_RS01580 at 294.966 kb on + strand, within MMP_RS01580 at 295.055 kb on + strand at 295.131 kb on + strand at 295.195 kb on + strand at 295.270 kb on + strand at 295.447 kb on + strand at 296.009 kb on + strand at 296.009 kb on + strand at 296.064 kb on - strand at 296.193 kb on - strand at 296.198 kb on + strand at 296.202 kb on - strand at 296.285 kb on - strand at 296.455 kb on - strand at 296.564 kb on - strand at 296.845 kb on - strand at 296.855 kb on - strand at 296.856 kb on + strand at 296.927 kb on - strand at 297.045 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 294,338 + +0.2 294,338 + -0.8 294,377 + MMP_RS01580 0.11 +0.5 294,377 + MMP_RS01580 0.11 +0.1 294,398 + MMP_RS01580 0.14 +0.4 294,398 + MMP_RS01580 0.14 -0.8 294,459 - MMP_RS01580 0.21 -2.0 294,470 + MMP_RS01580 0.22 +0.7 294,515 + MMP_RS01580 0.28 -0.4 294,637 + MMP_RS01580 0.42 +0.9 294,752 - MMP_RS01580 0.55 +0.0 294,753 + MMP_RS01580 0.56 -0.2 294,870 - MMP_RS01580 0.69 -0.1 294,966 + MMP_RS01580 0.81 -1.4 295,055 + -0.2 295,131 + +0.3 295,195 + -4.5 295,270 + -4.4 295,447 + -3.2 296,009 + -2.0 296,009 + -2.4 296,064 - -3.1 296,193 - +0.1 296,198 + -1.1 296,202 - -0.1 296,285 - -0.6 296,455 - -0.6 296,564 - -3.0 296,845 - -0.9 296,855 - -0.7 296,856 + -2.8 296,927 - -4.2 297,045 + +0.2
Or see this region's nucleotide sequence