Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01555 and MMP_RS01560 are separated by 247 nucleotides MMP_RS01560 and MMP_RS01565 are separated by 72 nucleotides MMP_RS01565 and MMP_RS01570 overlap by 4 nucleotides MMP_RS01570 and MMP_RS01575 are separated by 11 nucleotides
MMP_RS01555: MMP_RS01555 - hydrogenase formation protein HypD, at 289,876 to 290,958
_RS01555
MMP_RS01560: MMP_RS01560 - nascent polypeptide-associated complex protein, at 291,206 to 291,586
_RS01560
MMP_RS01565: MMP_RS01565 - AIR synthase related protein, at 291,659 to 293,002
_RS01565
MMP_RS01570: MMP_RS01570 - hypothetical protein, at 292,999 to 293,184
_RS01570
MMP_RS01575: MMP_RS01575 - aldolase, at 293,196 to 294,122
_RS01575
Position (kb) 291
292
293
294 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 290.676 kb on + strand at 290.697 kb on - strand at 290.700 kb on - strand at 290.958 kb on - strand at 290.959 kb on + strand at 291.003 kb on - strand at 291.003 kb on - strand at 291.003 kb on - strand at 291.003 kb on - strand at 291.006 kb on + strand at 291.008 kb on + strand at 291.013 kb on - strand at 291.036 kb on - strand at 291.036 kb on - strand at 291.037 kb on + strand at 291.042 kb on - strand at 291.054 kb on - strand at 291.055 kb on + strand at 291.139 kb on - strand at 291.140 kb on + strand at 291.146 kb on + strand at 291.163 kb on - strand at 291.168 kb on + strand at 291.172 kb on - strand at 291.187 kb on - strand at 291.191 kb on - strand at 291.674 kb on - strand at 291.807 kb on - strand at 291.854 kb on - strand at 291.855 kb on + strand at 292.597 kb on - strand at 292.620 kb on - strand at 293.157 kb on - strand, within MMP_RS01570 at 293.187 kb on - strand at 293.198 kb on + strand at 293.217 kb on - strand at 293.219 kb on - strand at 293.220 kb on + strand at 293.413 kb on + strand, within MMP_RS01575 at 293.420 kb on - strand, within MMP_RS01575 at 293.438 kb on - strand, within MMP_RS01575 at 293.487 kb on - strand, within MMP_RS01575 at 293.682 kb on - strand, within MMP_RS01575 at 293.682 kb on - strand, within MMP_RS01575 at 293.838 kb on + strand, within MMP_RS01575
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 290,676 + -2.7 290,697 - -2.9 290,700 - -2.7 290,958 - -0.6 290,959 + -3.3 291,003 - -1.1 291,003 - -0.6 291,003 - +0.5 291,003 - +0.6 291,006 + +0.7 291,008 + +0.4 291,013 - +0.8 291,036 - -0.7 291,036 - -1.1 291,037 + +0.2 291,042 - +1.0 291,054 - +0.1 291,055 + +0.3 291,139 - +0.9 291,140 + +0.1 291,146 + +0.1 291,163 - -2.0 291,168 + -0.3 291,172 - -0.2 291,187 - -3.6 291,191 - -1.0 291,674 - -2.0 291,807 - -4.3 291,854 - -3.7 291,855 + -1.3 292,597 - -4.0 292,620 - -3.1 293,157 - MMP_RS01570 0.85 +0.9 293,187 - +0.8 293,198 + +0.3 293,217 - +0.3 293,219 - -0.1 293,220 + +0.6 293,413 + MMP_RS01575 0.23 -0.8 293,420 - MMP_RS01575 0.24 +0.1 293,438 - MMP_RS01575 0.26 +0.7 293,487 - MMP_RS01575 0.31 +0.7 293,682 - MMP_RS01575 0.52 -0.4 293,682 - MMP_RS01575 0.52 -0.6 293,838 + MMP_RS01575 0.69 -1.4
Or see this region's nucleotide sequence