Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01530 and MMP_RS01535 are separated by 134 nucleotides MMP_RS01535 and MMP_RS01540 are separated by 9 nucleotides MMP_RS01540 and MMP_RS01545 are separated by 5 nucleotides
MMP_RS01530: MMP_RS01530 - translation initiation factor IF-2, at 284,636 to 286,441
_RS01530
MMP_RS01535: MMP_RS01535 - DHH family phosphoesterase, at 286,576 to 288,036
_RS01535
MMP_RS01540: MMP_RS01540 - DUF504 domain-containing protein, at 288,046 to 288,276
_RS01540
MMP_RS01545: MMP_RS01545 - CBS domain-containing protein, at 288,282 to 289,076
_RS01545
Position (kb) 286
287
288
289 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 285.874 kb on - strand at 286.119 kb on + strand at 286.263 kb on - strand at 286.296 kb on - strand at 286.296 kb on - strand at 286.483 kb on - strand at 286.484 kb on + strand at 286.485 kb on - strand at 286.486 kb on + strand at 286.500 kb on + strand at 286.506 kb on + strand at 286.544 kb on + strand at 286.768 kb on + strand at 286.832 kb on - strand at 286.846 kb on - strand at 286.848 kb on - strand at 286.849 kb on + strand at 286.868 kb on - strand at 286.911 kb on - strand at 287.494 kb on - strand at 287.494 kb on - strand at 287.768 kb on - strand at 287.779 kb on - strand at 287.781 kb on - strand at 287.782 kb on + strand at 287.813 kb on - strand at 287.841 kb on + strand at 287.841 kb on + strand at 288.044 kb on - strand at 288.104 kb on + strand, within MMP_RS01540 at 288.111 kb on + strand, within MMP_RS01540 at 288.590 kb on - strand, within MMP_RS01545 at 289.001 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 285,874 - -2.7 286,119 + -3.4 286,263 - -3.2 286,296 - -3.3 286,296 - -3.5 286,483 - -1.1 286,484 + -2.2 286,485 - -5.7 286,486 + -3.4 286,500 + -2.7 286,506 + -6.3 286,544 + -3.8 286,768 + -3.3 286,832 - -3.7 286,846 - -6.3 286,848 - -0.1 286,849 + -1.6 286,868 - -4.3 286,911 - -4.0 287,494 - -3.1 287,494 - -5.1 287,768 - -5.1 287,779 - -4.3 287,781 - -4.1 287,782 + -2.3 287,813 - -3.2 287,841 + -4.6 287,841 + -4.8 288,044 - -0.7 288,104 + MMP_RS01540 0.25 -0.2 288,111 + MMP_RS01540 0.28 -1.1 288,590 - MMP_RS01545 0.39 -3.2 289,001 - -0.9
Or see this region's nucleotide sequence