Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01390 and MMP_RS01395 are separated by 131 nucleotides MMP_RS01395 and MMP_RS01400 are separated by 87 nucleotides MMP_RS01400 and MMP_RS01405 are separated by 13 nucleotides MMP_RS01405 and MMP_RS01410 are separated by 178 nucleotides
MMP_RS01390: MMP_RS01390 - TATA-box-binding protein, at 258,939 to 259,484
_RS01390
MMP_RS01395: MMP_RS01395 - 50S ribosomal protein P1, at 259,616 to 259,915
_RS01395
MMP_RS01400: MMP_RS01400 - 50S ribosomal protein L10, at 260,003 to 261,010
_RS01400
MMP_RS01405: MMP_RS01405 - 50S ribosomal protein L1, at 261,024 to 261,665
_RS01405
MMP_RS01410: MMP_RS01410 - DNA-directed RNA polymerase subunit L, at 261,844 to 262,134
_RS01410
Position (kb) 260
261
262 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 259.147 kb on + strand at 259.525 kb on + strand at 259.903 kb on + strand at 259.936 kb on + strand at 259.992 kb on - strand at 259.995 kb on + strand at 260.284 kb on - strand at 260.986 kb on - strand at 261.114 kb on - strand at 261.403 kb on + strand at 261.724 kb on - strand at 261.833 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 259,147 + -1.9 259,525 + -4.1 259,903 + -1.1 259,936 + -2.9 259,992 - -3.2 259,995 + -0.4 260,284 - -3.1 260,986 - +0.7 261,114 - -3.1 261,403 + -2.8 261,724 - -2.1 261,833 - +1.2
Or see this region's nucleotide sequence