Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01385 and MMP_RS01390 are separated by 11 nucleotides MMP_RS01390 and MMP_RS01395 are separated by 131 nucleotides MMP_RS01395 and MMP_RS01400 are separated by 87 nucleotides
MMP_RS01385: MMP_RS01385 - imidazole glycerol phosphate synthase subunit HisH, at 258,316 to 258,927
_RS01385
MMP_RS01390: MMP_RS01390 - TATA-box-binding protein, at 258,939 to 259,484
_RS01390
MMP_RS01395: MMP_RS01395 - 50S ribosomal protein P1, at 259,616 to 259,915
_RS01395
MMP_RS01400: MMP_RS01400 - 50S ribosomal protein L10, at 260,003 to 261,010
_RS01400
Position (kb) 259
260 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 258.865 kb on - strand, within MMP_RS01385 at 258.908 kb on - strand at 258.943 kb on - strand at 258.974 kb on - strand at 258.993 kb on - strand at 258.997 kb on - strand at 258.998 kb on + strand at 258.998 kb on + strand at 259.147 kb on + strand at 259.525 kb on + strand at 259.903 kb on + strand at 259.936 kb on + strand at 259.992 kb on - strand at 259.995 kb on + strand at 260.284 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 258,865 - MMP_RS01385 0.90 -2.4 258,908 - -4.5 258,943 - -4.1 258,974 - -3.0 258,993 - -4.5 258,997 - -3.6 258,998 + -0.1 258,998 + -2.4 259,147 + -1.9 259,525 + -4.1 259,903 + -1.1 259,936 + -2.9 259,992 - -3.2 259,995 + -0.4 260,284 - -3.1
Or see this region's nucleotide sequence