Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01340 and MMP_RS01345 are separated by 7 nucleotides MMP_RS01345 and MMP_RS01350 overlap by 1 nucleotides MMP_RS01350 and MMP_RS01355 are separated by 54 nucleotides MMP_RS01355 and MMP_RS01360 are separated by 29 nucleotides MMP_RS01360 and MMP_RS01365 are separated by 98 nucleotides
MMP_RS01340: MMP_RS01340 - KEOPS complex Pcc1-like subunit, at 250,204 to 250,455
_RS01340
MMP_RS01345: MMP_RS01345 - rRNA maturation protein, at 250,463 to 250,957
_RS01345
MMP_RS01350: MMP_RS01350 - DNA-directed RNA polymerase subunit P, at 250,957 to 251,097
_RS01350
MMP_RS01355: MMP_RS01355 - 50S ribosomal protein L37Ae, at 251,152 to 251,442
_RS01355
MMP_RS01360: MMP_RS01360 - ribosome assembly factor SBDS, at 251,472 to 252,176
_RS01360
MMP_RS01365: MMP_RS01365 - archaeal proteasome endopeptidase complex subunit alpha, at 252,275 to 253,054
_RS01365
Position (kb)
251
252 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 250.181 kb on + strand at 250.192 kb on + strand at 250.202 kb on - strand at 250.206 kb on + strand at 250.226 kb on - strand at 250.226 kb on - strand at 250.227 kb on + strand at 250.260 kb on + strand at 250.265 kb on - strand at 250.458 kb on - strand at 250.603 kb on + strand at 250.606 kb on - strand at 250.811 kb on - strand at 250.811 kb on - strand at 250.812 kb on + strand at 250.818 kb on + strand at 250.977 kb on - strand at 250.993 kb on - strand at 251.491 kb on + strand at 251.495 kb on - strand at 252.199 kb on + strand at 252.389 kb on - strand, within MMP_RS01365
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 250,181 + -0.2 250,192 + -1.7 250,202 - +0.7 250,206 + -0.3 250,226 - -1.0 250,226 - -0.7 250,227 + -2.2 250,260 + -2.0 250,265 - -1.7 250,458 - -0.2 250,603 + +0.1 250,606 - -0.1 250,811 - -1.2 250,811 - +0.6 250,812 + -2.1 250,818 + +0.3 250,977 - +0.2 250,993 - -0.1 251,491 + -3.6 251,495 - -1.1 252,199 + -2.9 252,389 - MMP_RS01365 0.15 +0.8
Or see this region's nucleotide sequence