Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01230 and MMP_RS01235 are separated by 47 nucleotides MMP_RS01235 and MMP_RS01240 are separated by 15 nucleotides MMP_RS01240 and MMP_RS01245 are separated by 131 nucleotides
MMP_RS01230: MMP_RS01230 - glutamate-1-semialdehyde 2 1-aminomutase, at 231,125 to 232,408
_RS01230
MMP_RS01235: MMP_RS01235 - iron-containing alcohol dehydrogenase, at 232,456 to 233,460
_RS01235
MMP_RS01240: MMP_RS01240 - 7-cyano-7-deazaguanine synthase QueC, at 233,476 to 234,177
_RS01240
MMP_RS01245: MMP_RS01245 - anaerobic ribonucleoside-triphosphate reductase, at 234,309 to 236,621
_RS01245
Position (kb)
232
233
234 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 231.734 kb on + strand at 232.022 kb on + strand at 232.155 kb on + strand at 232.224 kb on - strand at 232.229 kb on - strand at 232.272 kb on + strand at 232.287 kb on - strand at 232.334 kb on + strand at 232.738 kb on + strand at 233.312 kb on - strand at 233.340 kb on + strand at 234.087 kb on - strand, within MMP_RS01240 at 234.094 kb on + strand, within MMP_RS01240 at 234.156 kb on - strand at 234.214 kb on + strand at 234.285 kb on + strand at 234.333 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 231,734 + -2.9 232,022 + -2.1 232,155 + -1.6 232,224 - -3.0 232,229 - -3.8 232,272 + -3.6 232,287 - -3.4 232,334 + -3.5 232,738 + -3.3 233,312 - -2.1 233,340 + -0.7 234,087 - MMP_RS01240 0.87 -0.2 234,094 + MMP_RS01240 0.88 -1.8 234,156 - -0.8 234,214 + -3.4 234,285 + +0.3 234,333 + +0.2
Or see this region's nucleotide sequence